Dielectrophoretic manipulation of particles for use in microfluidic devices
Amplification and hybridization of DNA are commonly used techniques to verify the presence of a specific DNA sequence in a test sample. Automatic sample handling to concentrate and purify sample prior to amplification is desirable both from the cost standpoint and from the standpoint of reducing the possibility of sample contamination. This paper explores the use of the dielectrophoretic force to manipulate DNA, Bacillus globigii spores, and Erwinia herbicola bacteria to provide concentration and purification as part of the sample handling functions in biological monitoring equipment. It was found that for what would be considered a typical microfabricated structure with electrode gaps at 30 {micro}m operating at 5V, that concentration of the particles is very effective.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE Office of Defense Programs (DP) (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 14568
- Report Number(s):
- UCRL-JC-133768; YN0100000; YN0100000; TRN: AH200129%%262
- Resource Relation:
- Conference: IMECE 299 Microfluidics Symposium, Nashville, TN (US), 11/14/1999--11/19/1999; Other Information: PBD: 23 Jun 1999
- Country of Publication:
- United States
- Language:
- English
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