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Title: Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome

Abstract

Microbial community assembly is affected by a combination of forces that act simultaneously, but the mechanisms underpinning their relative influences remain elusive. This gap strongly limits our ability to predict human impacts on microbial communities and the processes they regulate. Here, we experimentally demonstrate that increased salinity stress, food web alteration and nutrient loading interact to drive outcomes in salt marsh fungal leaf communities. Both salinity stress and food web alterations drove communities to deterministically diverge, resulting in distinct fungal communities. Increased nutrient loads, nevertheless, partially suppressed the influence of other factors as determinants of fungal assembly. Using a null model approach, we found that increased nutrient loads enhanced the relative importance of stochastic over deterministic divergent processes; without increased nutrient loads, samples from different treatments showed a relatively (deterministic) divergent community assembly whereas increased nutrient loads drove the system to more stochastic assemblies, suppressing the effect of other treatments. These results demonstrate that common anthropogenic modifications can interact to control fungal community assembly. As a result, our results suggest that when the environmental conditions are spatially heterogeneous (as in our case, caused by specific combinations of experimental treatments), increased stochasticity caused by greater nutrient inputs can reduce the importancemore » of deterministic filters that otherwise caused divergence, thus driving to microbial community homogenization.« less

Authors:
ORCiD logo [1];  [1];  [2]; ORCiD logo [3];  [1];  [1];  [4]
  1. Instituto de Investigaciones Marinas y Costeras (Argentina)
  2. Kansas State Univ., Manhattan, KS (United States)
  3. Kansas State Univ., Manhattan, KS (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Duke Univ., Beaufort, NC (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1456792
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Ecology
Additional Journal Information:
Journal Volume: 99; Journal Issue: 6; Journal ID: ISSN 0012-9658
Publisher:
Ecological Society of America (ESA)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; deterministic vs. neutral processes; leaf fungal communities; microbial community assembly; nutrient loading; salt marshes; Spartina

Citation Formats

Daleo, Pedro, Alberti, Juan, Jumpponen, Ari, Veach, Allison M., Ialonardi, Florencia, Iribarne, Oscar, and Silliman, Brian. Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome. United States: N. p., 2018. Web. doi:10.1002/ecy.2240.
Daleo, Pedro, Alberti, Juan, Jumpponen, Ari, Veach, Allison M., Ialonardi, Florencia, Iribarne, Oscar, & Silliman, Brian. Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome. United States. https://doi.org/10.1002/ecy.2240
Daleo, Pedro, Alberti, Juan, Jumpponen, Ari, Veach, Allison M., Ialonardi, Florencia, Iribarne, Oscar, and Silliman, Brian. 2018. "Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome". United States. https://doi.org/10.1002/ecy.2240. https://www.osti.gov/servlets/purl/1456792.
@article{osti_1456792,
title = {Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome},
author = {Daleo, Pedro and Alberti, Juan and Jumpponen, Ari and Veach, Allison M. and Ialonardi, Florencia and Iribarne, Oscar and Silliman, Brian},
abstractNote = {Microbial community assembly is affected by a combination of forces that act simultaneously, but the mechanisms underpinning their relative influences remain elusive. This gap strongly limits our ability to predict human impacts on microbial communities and the processes they regulate. Here, we experimentally demonstrate that increased salinity stress, food web alteration and nutrient loading interact to drive outcomes in salt marsh fungal leaf communities. Both salinity stress and food web alterations drove communities to deterministically diverge, resulting in distinct fungal communities. Increased nutrient loads, nevertheless, partially suppressed the influence of other factors as determinants of fungal assembly. Using a null model approach, we found that increased nutrient loads enhanced the relative importance of stochastic over deterministic divergent processes; without increased nutrient loads, samples from different treatments showed a relatively (deterministic) divergent community assembly whereas increased nutrient loads drove the system to more stochastic assemblies, suppressing the effect of other treatments. These results demonstrate that common anthropogenic modifications can interact to control fungal community assembly. As a result, our results suggest that when the environmental conditions are spatially heterogeneous (as in our case, caused by specific combinations of experimental treatments), increased stochasticity caused by greater nutrient inputs can reduce the importance of deterministic filters that otherwise caused divergence, thus driving to microbial community homogenization.},
doi = {10.1002/ecy.2240},
url = {https://www.osti.gov/biblio/1456792}, journal = {Ecology},
issn = {0012-9658},
number = 6,
volume = 99,
place = {United States},
year = {Thu Apr 12 00:00:00 EDT 2018},
month = {Thu Apr 12 00:00:00 EDT 2018}
}

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Cited by: 8 works
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Works referenced in this record:

UCHIME improves sensitivity and speed of chimera detection
journal, June 2011


Microbial diversity in the deep sea and the underexplored "rare biosphere"
journal, July 2006


Using null models to disentangle variation in community dissimilarity from variation in α-diversity
journal, February 2011


Culturing of ‘unculturable’ human microbiota reveals novel taxa and extensive sporulation
journal, May 2016


Physical stress modifies top-down and bottom-up forcing on plant growth and reproduction in a coastal ecosystem
journal, August 2015


Stochastic and deterministic assembly processes in subsurface microbial communities
journal, March 2012


Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007


Herbivore exclusion promotes a more stochastic plant community assembly in a natural grassland
journal, March 2017


Patterns of fungal diversity and composition along a salinity gradient
journal, September 2010


Ironing out the wrinkles in the rare biosphere through improved OTU clustering: Ironing out the wrinkles in the rare biosphere
journal, March 2010


Resistance, resilience, and redundancy in microbial communities
journal, August 2008


The UNITE database for molecular identification of fungi - recent updates and future perspectives: Letters
journal, March 2010


Competition, Not Cooperation, Dominates Interactions among Culturable Microbial Species
journal, October 2012


Conceptual Synthesis in Community Ecology
journal, June 2010


Assessing the relative importance of neutral stochasticity in ecological communities
journal, September 2014


Pyrosequencing enumerates and contrasts soil microbial diversity
journal, July 2007


Disentangling mechanisms that mediate the balance between stochastic and deterministic processes in microbial succession
journal, March 2015


Herbivores and nutrients control grassland plant diversity via light limitation
journal, March 2014


New primers to amplify the fungal ITS2 region - evaluation by 454-sequencing of artificial and natural communities
journal, July 2012


Influence of Phylogeny on Fungal Community Assembly and Ecosystem Functioning
journal, June 2007


Disentangling the importance of ecological niches from stochastic processes across scales
journal, August 2011


Multivariate dispersion as a measure of beta diversity
journal, June 2006


Contrasting primary successional trajectories of fungi and bacteria in retreating glacier soils
journal, September 2013


Drought mediates the importance of stochastic community assembly
journal, October 2007


Stochasticity, succession, and environmental perturbations in a fluidic ecosystem
journal, February 2014


Biogeographic patterns in below-ground diversity in New York City's Central Park are similar to those observed globally
journal, November 2014


A Comparison of some methods for analysing changes in benthic community structure
journal, February 1991


Combined niche and neutral effects in a microbial wastewater treatment community
journal, August 2010


The role of ecological theory in microbial ecology
journal, May 2007


Neutral assembly of bacterial communities: Neutral assembly of bacterial communities
journal, November 2007


Effects of nutrient enrichment and crab herbivory on a SW Atlantic salt marsh productivity
journal, August 2011


Increasing aridity reduces soil microbial diversity and abundance in global drylands
journal, December 2015


The ecology of the microbiome: Networks, competition, and stability
journal, November 2015


Competition for Light Causes Plant Biodiversity Loss After Eutrophication
journal, April 2009


Dispersal enhances beta diversity in nectar microbes
journal, June 2017


Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009


Bacterial diversity and community composition from seasurface to subseafloor
journal, October 2015


Quantifying community assembly processes and identifying features that impose them
journal, June 2013


Challenges in microbial ecology: building predictive understanding of community function and dynamics
journal, March 2016


Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
journal, March 2012


Dispersal alters bacterial diversity and composition in a natural community
journal, October 2017


ENSO episodes modify plant/terrestrial–herbivore interactions in a southwestern Atlantic salt marsh
journal, December 2010


Abiotic stress mediates top-down and bottom-up control in a Southwestern Atlantic salt marsh
journal, November 2009


The diversity and biogeography of soil bacterial communities
journal, January 2006


Spatial and Resource Factors Influencing High Microbial Diversity in Soil
journal, January 2002


Effects of dispersal and selection on stochastic assembly in microbial communities
journal, August 2016


Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies
journal, December 2011


Competition for light causes plant biodiversity loss after eutrophication
text, January 2009


Challenges in microbial ecology: building predictive understanding of community function and dynamics.
text, January 2016


Works referencing / citing this record:

Environmental filtering drives bacterial community structure and function in a subalpine area of northern China
journal, December 2018