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Title: HLB1 Is a Tetratricopeptide Repeat Domain-Containing Protein That Operates at the Intersection of the Exocytic and Endocytic Pathways at the TGN/EE in Arabidopsis

Journal Article · · The Plant Cell
DOI:https://doi.org/10.1105/tpc.15.00794· OSTI ID:1438269
 [1]; ORCiD logo [1];  [2];  [3];  [2]; ORCiD logo [1]
  1. The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Plant Biology Division
  2. Michigan State Univ., East Lansing, MI (United States). Plant Research Lab.
  3. The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Computing Services Dept.
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The endomembrane system plays essential roles in plant development, but the proteome responsible for its function and organization remains largely uncharacterized in plants. For this study, we identified and characterized the HYPERSENSITIVE TO LATRUNCULIN B1 (HLB1) protein isolated through a forward-genetic screen in Arabidopsis thaliana for mutants with heightened sensitivity to actin-disrupting drugs. HLB1 is a plant-specific tetratricopeptide repeat domain-containing protein of unknown function encoded by a single Arabidopsis gene. HLB1 associated with the trans-Golgi network (TGN)/early endosome (EE) and tracked along filamentous actin, indicating that it could link post-Golgi traffic with the actin cytoskeleton in plants. HLB1 was found to interact with the ADP-ribosylation-factor guanine nucleotide exchange factor, MIN7/BEN1 (HOPM INTERACTOR7/BREFELDIN A-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE1) by coimmunoprecipitation. The min7/ben1 mutant phenocopied the mild root developmental defects and latrunculin B hypersensitivity of hlb1, and analyses of a hlb1/ min7/ben1 double mutant showed that hlb1 and min7/ben1 operate in common genetic pathways. Based on these data, we propose that HLB1 together with MIN7/BEN1 form a complex with actin to modulate the function of the TGN/EE at the intersection of the exocytic and endocytic pathways in plants.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States); Michigan State Univ., East Lansing, MI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Aeronautics and Space Administration (NASA)
Grant/Contract Number:
FC02-07ER64494; FG02-91ER20021; NNX12AM94G
OSTI ID:
1438269
Journal Information:
The Plant Cell, Vol. 28, Issue 3; ISSN 1040-4651
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 20 works
Citation information provided by
Web of Science

Cited By (8)

An EMS-induced mutation in a tetratricopeptide repeat-like superfamily protein gene (Ghir_A12G008870) on chromosome A12 is responsible for the liy short fiber phenotype in cotton journal October 2019
TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network journal December 2018
AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis journal August 2019
Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking journal January 2019
Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein journal May 2018
TDNAscan: A Software to Identify Complete and Truncated T-DNA Insertions journal July 2019
Direct Comparison of the Performance of Commonly Employed In Vivo F-actin Markers (Lifeact-YFP, YFP-mTn and YFP-FABD2) in Tobacco Pollen Tubes journal August 2017
Regulation of Small RNAs and Corresponding Targets in Nod Factor-Induced Phaseolus vulgaris Root Hair Cells journal June 2016

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