Bio-Oxo Technology
- Easel Biotechnologies, LLC, Culver City, CA (United States)
Easel Biotechnologies, LLC’s Bio-Oxo process has demonstrated that isobutyraldehyde can be biologically produced from corn stover hydrolysate up to 56 g/L in a 14L fermentor. This was accomplished by metabolically engineering bacterial strains to not only produce isobutyraldehyde, but to do so by co-utilizing corn stover hydrolysate sugars, glucose and xylose. Also essential to the success of the Bio-Oxo process was that it utilized gas stripping as a means of product separation, allowing for the continuous removal of isobutyraldehyde. This aided in not only reducing energy costs associated with separation, but also alleviating product toxicity, resulting in higher production. Although we were not able to complete our economic analysis based on pilot scale fermentations, the improvements we have made from strain engineering to product separation, should result in the reduced cost of isobutyraldehyde. Still, as the project has ended prematurely, there is room for additional optimization. Improvements in productivity and sugar utilization would result in a further reduction in capital and recovery costs. As a biological-based process, the utilization of corn stover results in reduced greenhouse gas emissions as compared to petroleum-based chemical synthesis. In addition, as a true replacement chemical “drop in” system, no downstream production units need to be changed. Jobs can also be created as farm waste needs to be collected and transported to the new production facility.
- Research Organization:
- Easel Biotechnologies, LLC, Culver City, CA (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office
- DOE Contract Number:
- EE0005773
- OSTI ID:
- 1437008
- Report Number(s):
- DOE-Easel-EE0005773
- Country of Publication:
- United States
- Language:
- English
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