Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB

Journal Article · Wed Jan 10 00:00:00 EST 2018 · Journal of Structural Biology

DOI:https://doi.org/10.1016/j.jsb.2018.01.006· OSTI ID:1435826

Gokey, Trevor ^[1]; Halavaty, Andrei S. ^[2]; Minasov, George ^[2]; Anderson, Wayne F. ^[2]; Kuhn, Misty L. ^[1]

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1. San Francisco State Univ., CA (United States)
2. Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States); Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)

Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. In this work, we determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: Startup Funds from San Francisco State University; National Institute of Allergy and Infectious Diseases (NIAID); National Institutes of Health (NIH); US Department of Health and Human Services (HHS)
• Grant/Contract Number: HHSN272200700058C; HHSN272201200026C
• OSTI ID: 1435826
• Journal Information: Journal of Structural Biology, Vol. 202, Issue 2; ISSN 1047-8477
• Publisher: Elsevier
• Country of Publication: United States
• Language: ENGLISH

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