Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production

Liu, Fushan; Zhao, Qianru; Mano, Noel; Ahmed, Zaheer; Nitschke, Felix; Cai, Yinqqi; Chapman, Kent D.; Steup, Martin; Tetlow, Ian J.; Emes, Michael J.

doi:10.1111/pbi.12453

Title: Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production

Journal Article · Wed Aug 19 00:00:00 EDT 2015 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12453· OSTI ID:1435780

Liu, Fushan ^[1]; Zhao, Qianru ^[1]; Mano, Noel ^[1]; Ahmed, Zaheer ^[1]; Nitschke, Felix ^[2]; Cai, Yinqqi ^[3]; Chapman, Kent D. ^[3]; Steup, Martin ^[1]; Tetlow, Ian J. ^[1]; Emes, Michael J. ^[1]

Univ. of Guelph, Guelph, ON (Canada)
The Hospital for Sick Children, Toronto, ON (Canada)
Univ. of North Texas, Denton, TX (United States)

Here, we have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm–expressed maize (Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch–free background and with the wild–type plants. Each of the maize–derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more–than–trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0000797

OSTI ID:: 1435780

Journal Information:: Plant Biotechnology Journal, Vol. 14, Issue 3; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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