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Title: In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Journal Article · · Journal of Applied Oral Science
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  1. Sao Paulo State Univ. (UNESP), Aracatuba (Brazil). Faculty of Dentistry of Aracatuba. Dept. of Child and Social Dentistry
  2. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Dept. of Molecular Pharmacology and Biological Chemistry
  3. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Ann and Robert Lurie Children's Hospital
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
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Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States); Sao Paulo State Univ. (UNESP), Aracatuba (Brazil)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1435039
Journal Information:
Journal of Applied Oral Science, Vol. 25, Issue 6; ISSN 1678-7757
Publisher:
SciELOCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 8 works
Citation information provided by
Web of Science

References (15)

Comparison of Methods for Evaluating Mineral Loss: Hardness versus Synchrotron Microcomputed Tomography journal January 2009
The Inhibitory Effect of MI Paste, Fluoride and a Combination of Both on the Progression of Artificial Caries-like Lesions in Enamel journal September 2008
Fluoride Dose Response in pH-Cycling Models Using Bovine Enamel journal January 2005
Performance of a double-multilayer monochromator at Beamline 2-BM at the Advanced Photon Source journal March 2002
Effect of rinsing with water immediately after neutral gel and foam fluoride topical application on enamel remineralization: An in situ study journal November 2010
Effect of Fluoride, Lesion Baseline Severity and Mineral Distribution on Lesion Progression journal January 2012
Evaluation of Laser Fluorescence in the Monitoring of the Initial Stage of the De-/Remineralization Process: An in vitro and in situ Study journal January 2009
NMR studies of a novel calcium, phosphate and fluoride delivery vehicle-αS1-casein(59–79) by stabilized amorphous calcium fluoride phosphate nanocomplexes journal September 2004
Calcium phosphate-based remineralization systems: scientific evidence? journal September 2008
Quantitative analysis of mineral content in enamel using synchrotron microtomography and microhardness analysis conference August 2006
Effect of fluoride gels supplemented with sodium trimetaphosphate in reducing demineralization journal September 2013
Acid Susceptibility at Various Depths of pH-Cycled Enamel and Dentine Specimens journal December 2005
Enamel Subsurface Lesion Remineralisation with Casein Phosphopeptide Stabilised Solutions of Calcium, Phosphate and Fluoride journal January 2008
Amorphous calcium phosphate and its application in dentistry journal July 2011
pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization journal January 2008

Cited By (1)

The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion journal January 2019

Linked Research (2)

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Related Subjects

60 APPLIED LIFE SCIENCES
X-ray microtomography
tooth remineralization
calcium phosphates
fluoride
dental enamel