Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- gene/Networks, Inc., Alameda, CA (United States)
The ultimate goal of this project is to identify the human gene( s) responsible for the disorder known as IBD. As a first step towards this goal, the Parties agreed to carry out experiments to c011fim1 and refine linkage of IBD to the chromosomal region( s) of interest identified by gene/Networks, Inc. If results confirmed association of these region(s) with the disease, the Parties planned to complete a physical map of the genetically defined region(s). The work was planned in two phases. The desired products resulting from Phase I were BAC clone(s) containing the genetic marker(s) identified by gene\Networks, Inc. as potentially linked to IBD, plasmid subclones of those BAC(s), and new genetic markers developed from these plasmid subclones. The newly developed markers would be genotyped by gene/Networks, Inc. to ascertain evidence for linkage or non-linkage of IBD to this region. If non-linkage was indicated, the project would move to investigation of other candidate chromosomal regions. Where linkage was indicated, the project would move to Phase II, in which a physical map of the candidate region(s) would be developed. The products of this phase would be contig(s) of BAC clones in the region exhibiting linkage to IBD, as well as plasmid subclones of the BACs and further genetic marker development. There would also be continued genotyping with new polymorphic markers during this phase. It was anticipated that clones identified and developed during these two phases would provide the physical resources for eventual disease gene discovery.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC52-07NA27344
- OSTI ID:
- 1430922
- Report Number(s):
- LLNL-TR-747675
- Country of Publication:
- United States
- Language:
- English
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