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Title: Instructing cells with programmable peptide DNA hybrids

Journal Article · · Nature Communications
DOI:https://doi.org/10.1038/ncomms15982· OSTI ID:1429073
 [1];  [1];  [1];  [2];  [1];  [3]; ORCiD logo [1];  [1];  [4]
  1. Northwestern Univ., Chicago, IL (United States). Simpson Querrey Inst. for BioNanotechnology
  2. Northwestern Univ., Evanston, IL (United States). Dept. of Biomedical Engineering
  3. Northwestern Univ., Evanston, IL (United States). Dept. of Materials Science and Engineering
  4. Northwestern Univ., Chicago, IL (United States). Simpson Querrey Inst. for BioNanotechnology. Dept. of Medicine; Northwestern Univ., Evanston, IL (United States). Dept. of Biomedical Engineering. Dept. of Materials Science and Engineering. Dept. of Chemistry
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The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.

Research Organization:
Northwestern Univ., Evanston, IL (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Bio-Inspired Energy Science (CBES)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Science Foundation (NSF); European Molecular Biology Organization (EMBO)
Grant/Contract Number:
FG02-00ER45810; SC0000989; 5R01DE015920; 1F32NS077728-01A1; CCSG P30 CA060553; DGE-1324585; NNCI-1542205; DMR-1121262; ALTF 233-2012
OSTI ID:
1429073
Alternate ID(s):
OSTI ID: 1821885; OSTI ID: 1846797
Journal Information:
Nature Communications, Vol. 8; ISSN 2041-1723
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 60 works
Citation information provided by
Web of Science

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Cited By (10)

Biomedical Applications of DNA‐Based Hydrogels journal November 2019
Emerging applications of peptide–oligonucleotide conjugates: bioactive scaffolds, self-assembling systems, and hybrid nanomaterials journal January 2019
Bioengineering strategies to accelerate stem cell therapeutics journal May 2018
About Chemical Strategies to Fabricate Cell-Instructive Biointerfaces with Static and Dynamic Complexity journal May 2018
3D printing with peptide–polymer conjugates for single-step fabrication of spatially functionalized scaffolds journal January 2019
Programmable Construction of Peptide‐Based Materials in Living Subjects: From Modular Design and Morphological Control to Theranostics journal November 2018
Grafting from a Hybrid DNA–Covalent Polymer by the Hybridization Chain Reaction journal July 2018
Supramolecular Self‐Assembly of DNA with a Cationic Polythiophene: From Polyplexes to Fibers journal February 2019
Biomedical Applications of DNA-Based Hydrogels text January 2019
Manipulating cell fate: dynamic control of cell behaviors on functional platforms journal January 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
36 MATERIALS SCIENCE
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biomaterials - cells
biomedical materials
99 GENERAL AND MISCELLANEOUS
Bioinspired materials Biomaterials – cells Biomedical materials