Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Thomas, Vanessa A.; Donohoe, Bryon S.; Li, Mi; Pu, Yunqiao; Ragauskas, Arthur J.; Kumar, Rajeev; Nguyen, Thanh Yen; Cai, Charles M.; Wyman, Charles E.

doi:10.1186/s13068-017-0937-3

Title: Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Journal Article · Thu Nov 30 00:00:00 EST 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0937-3· OSTI ID:1412036

Thomas, Vanessa A. ^[1]; Donohoe, Bryon S. ^[2]; Li, Mi ^[3]; Pu, Yunqiao ^[3]; Ragauskas, Arthur J. ^[4]; Kumar, Rajeev ^[1]; Nguyen, Thanh Yen ^[5]; Cai, Charles M. ^[1];

^[1]

Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of California, Riverside, CA (United States)

Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies. This study focused on determining the effect of pretreatment by dilute sulfuric acid alone (DA) and with tetrahydrofuran (THF) addition via co-solvent-enhanced lignocellulosic fractionation (CELF) on deconstruction of corn stover and Populus with much different recalcitrance by C. thermocellum vs. fungal enzymes and changes in pretreated biomass related to these differences.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308

OSTI ID:: 1412036

Alternate ID(s):: OSTI ID: 1414067

Report Number(s):: NREL/JA-2700-70675; 106233

Journal Information:: Biotechnology for Biofuels, Vol. 10, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 31 works

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09 BIOMASS FUELS
consolidated bioprocessing
recalcitrance
yield
sugar
fractionation
tetrahydrofuran

MOESM1 of Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.5648746.v1 The current record is supplemented by this dataset	dataset	November 2017
Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes [Supplemental Data] Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.c.3942298 The current record is supplemented by this dataset	dataset	November 2017
Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.c.3942298.v1 The current record is supplemented by this dataset	dataset	November 2017
MOESM1 of Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.5648746 The current record is supplemented by this text	text	January 2017