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Title: Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates

Abstract

Coiled-coil peptide-polymer conjugates are an emerging class of biomaterials. Fundamental understanding of the coiled-coil oligomeric state and assembly process of these hybrid building blocks is necessary to exert control over their assembly into well-defined structures. Here in this paper, we studied the effect of peptide structure and PEGylation on the self-assembly process and oligomeric state of a Langmuir monolayer of amphiphilic coiled-coil peptide-polymer conjugates using X-ray reflectivity (XR) and grazing-incidence X-ray diffraction (GIXD). Our results show that the oligomeric state of PEGylated amphiphiles based on 3-helix bundle-forming peptide is surface pressure dependent, a mixture of dimers and trimers was formed at intermediate surface pressure but transitions into trimers completely upon increasing surface pressure. Moreover, the interhelical distance within the coiled-coil bundle of 3-helix peptide-PEG conjugate amphiphiles was not perturbed under high surface pressure. Present studies provide valuable insights into the self-assembly process of hybrid peptide-polymer conjugates and guidance to develop biomaterials with controlled multivalency of ligand presentation.

Authors:
 [1];  [2];  [2];  [3]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Materials Science and Engineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division
  2. Univ. of California, Berkeley, CA (United States). Dept. of Materials Science and Engineering
  3. Univ. of California, Berkeley, CA (United States). Dept. of Materials Science and Engineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1411562
Grant/Contract Number:  
AC02-05CH11231; 5R21EB016947-02; CHE-1346572; AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biomacromolecules
Additional Journal Information:
Journal Volume: 17; Journal Issue: 12; Journal ID: ISSN 1525-7797
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Lund, Reidar, Ang, JooChuan, Shu, Jessica Y., and Xu, Ting. Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates. United States: N. p., 2016. Web. doi:10.1021/acs.biomac.6b01356.
Lund, Reidar, Ang, JooChuan, Shu, Jessica Y., & Xu, Ting. Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates. United States. https://doi.org/10.1021/acs.biomac.6b01356
Lund, Reidar, Ang, JooChuan, Shu, Jessica Y., and Xu, Ting. 2016. "Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates". United States. https://doi.org/10.1021/acs.biomac.6b01356. https://www.osti.gov/servlets/purl/1411562.
@article{osti_1411562,
title = {Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates},
author = {Lund, Reidar and Ang, JooChuan and Shu, Jessica Y. and Xu, Ting},
abstractNote = {Coiled-coil peptide-polymer conjugates are an emerging class of biomaterials. Fundamental understanding of the coiled-coil oligomeric state and assembly process of these hybrid building blocks is necessary to exert control over their assembly into well-defined structures. Here in this paper, we studied the effect of peptide structure and PEGylation on the self-assembly process and oligomeric state of a Langmuir monolayer of amphiphilic coiled-coil peptide-polymer conjugates using X-ray reflectivity (XR) and grazing-incidence X-ray diffraction (GIXD). Our results show that the oligomeric state of PEGylated amphiphiles based on 3-helix bundle-forming peptide is surface pressure dependent, a mixture of dimers and trimers was formed at intermediate surface pressure but transitions into trimers completely upon increasing surface pressure. Moreover, the interhelical distance within the coiled-coil bundle of 3-helix peptide-PEG conjugate amphiphiles was not perturbed under high surface pressure. Present studies provide valuable insights into the self-assembly process of hybrid peptide-polymer conjugates and guidance to develop biomaterials with controlled multivalency of ligand presentation.},
doi = {10.1021/acs.biomac.6b01356},
url = {https://www.osti.gov/biblio/1411562}, journal = {Biomacromolecules},
issn = {1525-7797},
number = 12,
volume = 17,
place = {United States},
year = {Wed Oct 26 00:00:00 EDT 2016},
month = {Wed Oct 26 00:00:00 EDT 2016}
}

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Works referenced in this record:

Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors
journal, November 1998


Carbohydrate-Protein Interactions: Basis of Glycobiology
journal, August 1995


The Cluster Glycoside Effect
journal, February 2002


Influencing Receptor−Ligand Binding Mechanisms with Multivalent Ligand Architecture
journal, December 2002


Activation of Integrin Function by Nanopatterned Adhesive Interfaces
journal, March 2004


Mechanisms of integrin activation and trafficking
journal, October 2011


Biodendrimer-Based Hydrogel Scaffolds for Cartilage Tissue Repair
journal, January 2006


Three-Dimensional Biochemical and Biomechanical Patterning of Hydrogels for Guiding Cell Behavior
journal, October 2006


Multivalent Integrin-Specific Ligands Enhance Tissue Healing and Biomaterial Integration
journal, August 2010


Multivalent dendrimers presenting spatially controlled clusters of binding epitopes in thermoresponsive hyaluronan hydrogels
journal, October 2014


Ligand-Clustered “Patchy” Nanoparticles for Modulated Cellular Uptake and In Vivo Tumor Targeting
journal, August 2010


Effect of ligand density, receptor density, and nanoparticle size on cell targeting
journal, February 2013


From Phage Display to Nanoparticle Delivery: Functionalizing Liposomes with Multivalent Peptides Improves Targeting to a Cancer Biomarker
journal, December 2012


New Design of Helix Bundle Peptide−Polymer Conjugates
journal, August 2008


Solution Structural Characterization of Coiled-Coil Peptide–Polymer Side-Conjugates
journal, May 2012


Long-Circulating 15 nm Micelles Based on Amphiphilic 3-Helix Peptide–PEG Conjugates
journal, May 2012


3-Helix Micelles Stabilized by Polymer Springs
journal, July 2012


Evaluation of Doxorubicin-Loaded 3-Helix Micelles as Nanocarriers
journal, September 2013


Self-assembled 20-nm 64Cu-micelles enhance accumulation in rat glioblastoma
journal, December 2015


Controlled formation of model homo- and heterodimer coiled coil polypeptides
journal, November 1993


Specific sequences from the carboxyl terminus of human p53 gene product form anti-parallel tetramers in solution.
journal, September 1994


Reversible Self-Organization of Poly(ethylene glycol)-Based Hybrid Block Copolymers Mediated by a De Novo Four-Stranded α-Helical Coiled Coil Motif
journal, June 2003


Self-assembly of designed coiled coil peptides studied by small-angle X-ray scattering and analytical ultracentrifugation: SAXS OF COILED COILS
journal, March 2013


Fifty years of coiled-coils and α-helical bundles: A close relationship between sequence and structure
journal, September 2008


X-Ray Diffraction Studies of Organic Monolayers on the Surface of Water
journal, May 1987


Ordering in Lipid Monolayers Studied by Synchrotron X-Ray Diffraction and Fluorescence Microscopy
journal, May 1987


THE CONSTITUTION AND FUNDAMENTAL PROPERTIES OF SOLIDS AND LIQUIDS. II. LIQUIDS. 1
journal, September 1917


X-ray reflectivity of a Langmuir monolayer on water
journal, January 1987


X-ray and neutron reflectivity from spread monolayers
journal, May 1988


Phase diagram of a lipid monolayer on the surface of water
journal, July 1990


Two-Dimensional Crystallography of Amphiphilic Molecules at the Air–Water Interface
journal, February 1992


X-ray Diffraction Study of a Recently Identified Phase Transition in Fatty Acid Langmuir Monolayers
journal, February 1994


The structure of a Langmuir monolayer of methyl eicosanoate as determined by x‐ray diffraction and Brewster angle microscopy
journal, August 1996


Amphiphilic Four-Helix Bundle Peptides Designed for Light-Induced Electron Transfer Across a Soft Interface
journal, September 2005


The liquid surface/interface spectrometer at ChemMatCARS synchrotron facility at the Advanced Photon Source
journal, August 2003


Stochastic fitting of specular X-ray reflectivity data using StochFit
journal, November 2008


Sound velocity and hydration phenomena in aqueous polymeric solutions
journal, February 1993


Self-assembly of short amyloidogenic peptides at the air–water interface
journal, August 2011


Symmetry, shape, and order
journal, November 2007


X-ray Reflectivity of Diblock Copolymer Monolayers at the Air/Water Interface
journal, December 1995


Neutron and X-ray reflectometry of interfacial systems in colloid and polymer chemistry
journal, February 1996


Phase Transitions in Monolayers of PS−PEO Copolymer at the Air−Water Interface
journal, December 1999


Conformational and Aggregation Properties of a PEGylated Alanine-Rich Polypeptide
journal, June 2011


Soft Hydrogels from Nanotubes of Poly(ethylene oxide)−Tetraphenylalanine Conjugates Prepared by Click Chemistry
journal, January 2009


The Effect of PEO Length on the Self-Assembly of Poly(ethylene oxide)−Tetrapeptide Conjugates Prepared by “Click” Chemistry
journal, August 2009


Works referencing / citing this record: