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Title: Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells

Journal Article · · Microscopy and Microanalysis
 [1];  [2];  [1];  [2];  [3];  [1];  [4];  [5];  [1]
  1. Argonne National Lab. (ANL), Argonne, IL (United States)
  2. Northwestern Univ., Evanston, IL (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Northwestern Univ., Argonne, IL (United States)
  5. Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
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Bionanoprobe (BNP), a hard x-ray fluorescence sample-scanning nanoprobe at the Advanced Photon Source of Argonne National Laboratory, has been used to quantitatively study elemental distributions in biological cells with sub-100 nm spatial resolution and high sensitivity. Cryogenic conditions enable biological samples to be studied in their frozen-hydrated state with both ultrastructure and elemental distributions more faithfully preserved compared to conventional chemical fixation or dehydration methods. Furthermore, radiation damage is reduced in two ways: the diffusion rate of free radicals is decreased at low temperatures; and the sample is embedded in vitrified ice, which reduces mass loss.

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Organization:
National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1411022
Journal Information:
Microscopy and Microanalysis, Vol. 22, Issue S3; ISSN 1431-9276
Publisher:
Microscopy Society of America (MSA)Copyright Statement
Country of Publication:
United States
Language:
English

References (8)

Zernike phase contrast in scanning microscopy with X-rays journal September 2010
Continuous motion scan ptychography: characterization for increased speed in coherent x-ray imaging journal January 2015
Reconstructing state mixtures from diffraction measurements journal February 2013
Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy journal January 2000
The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities journal December 2013
Radiation processes in frozen aqueous systems journal January 1982
Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae journal February 2015
Atomic radiative and radiationless yields for K and L shells journal April 1979

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES