BET N-terminal bromodomain inhibition selectively blocks Th17 cell differentiation and ameliorates colitis in mice

Cheung, Kalung; Lu, Geming; Sharma, Rajal; Vincek, Adam; Zhang, Ruihua; Plotnikov, Alexander N.; Zhang, Fan; Zhang, Qiang; Ju, Ying; Hu, Yuan; Zhao, Li; Han, Xinye; Meslamani, Jamel; Xu, Feihong; Jaganathan, Anbalagan; Shen, Tong; Zhu, Hongfa; Rusinova, Elena; Zeng, Lei; Zhou, Jiachi; Yang, Jianjun; Peng, Liang; Ohlmeyer, Michael; Walsh, Martin J.; Zhang, David Y.; Xiong, Huabao; Zhou, Ming-Ming

doi:10.1073/pnas.1615601114

Title: BET N-terminal bromodomain inhibition selectively blocks Th17 cell differentiation and ameliorates colitis in mice

Journal Article · Mon Mar 06 00:00:00 EST 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1615601114· OSTI ID:1409528

Cheung, Kalung ^[1]; Lu, Geming ^[1]; Sharma, Rajal ^[1]; Vincek, Adam ^[1]; Zhang, Ruihua ^[1]; Plotnikov, Alexander N. ^[1]; Zhang, Fan ^[1]; Zhang, Qiang ^[2]; Ju, Ying ^[3]; Hu, Yuan ^[1]; Zhao, Li ^[3]; Han, Xinye ^[3]; Meslamani, Jamel ^[1]; Xu, Feihong ^[1]; Jaganathan, Anbalagan ^[1]; Shen, Tong ^[1]; Zhu, Hongfa ^[1]; Rusinova, Elena ^[1]; Zeng, Lei ^[2]; Zhou, Jiachi ^[1] more »

Icahn School of Medicine at Mount Sinai, New York, NY (United States)
Icahn School of Medicine at Mount Sinai, New York, NY (United States); The First Hospital of Jilin Univ., Changchun (China)
The First Hospital of Jilin Univ., Changchun (China)

T-helper 17 (Th17) cells have important functions in adaptor immunity and have also been implicated in inflammatory disorders. The bromodomain and extraterminal domain (BET) family proteins regulate gene transcription during lineage-specific differentiation of naïve CD4⁺T cells to produce mature T-helper cells. Inhibition of acetyl-lysine binding of the BET proteins by pan-BET bromodomain (BrD) inhibitors, such as JQ1, broadly affects differentiation of Th17, Th1, and Th2 cells that have distinct immune functions, thus limiting their therapeutic potential. Whether these BET proteins represent viable new epigenetic drug targets for inflammatory disorders has remained an unanswered question. In this study, we report that selective inhibition of the first bromodomain of BET proteins with our newly designed small molecule MS402 inhibits primarily Th17 cell differentiation with a little or almost no effect on Th1 or Th2 and Treg cells. MS402 preferentially renders Brd4 binding to Th17 signature gene loci over those of housekeeping genes and reduces Brd4 recruitment of p-TEFb to phosphorylate and activate RNA polymerase II for transcription elongation. Furthermore, we show that MS402 prevents and ameliorates T-cell transfer-induced colitis in mice by blocking Th17 cell overdevelopment. Thus, selective pharmacological modulation of individual bromodomains likely represents a strategy for treatment of inflammatory bowel diseases.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); First Hospital of Jilin University; National Natural Science Foundation of China (NSFC); National Institutes of Health (NIH)

Grant/Contract Number:: SC0012704; 81601409

OSTI ID:: 1409528

Report Number(s):: BNL-114580-2017-JA

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 11; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 69 works

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Computational study on the selective inhibition mechanism of MS402 to the first and second bromodomains of BRD4 Wang, Qianqian; Li, Ying; Xu, Jiahui Proteins: Structure, Function, and Bioinformatics, Vol. 87, Issue 1 https://doi.org/10.1002/prot.25611	journal	November 2018
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60 APPLIED LIFE SCIENCES
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bromodomain
chemical inhibitor

Title: BET N-terminal bromodomain inhibition selectively blocks Th17 cell differentiation and ameliorates colitis in mice

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