Structural insights into the light-driven auto-assembly process of the water-oxidizing Mn4CaO5-cluster in photosystem II

Zhang, Miao; Bommer, Martin; Chatterjee, Ruchira; Hussein, Rana; Yano, Junko; Dau, Holger; Kern, Jan; Dobbek, Holger; Zouni, Athina

doi:10.7554/eLife.26933

Title: Structural insights into the light-driven auto-assembly process of the water-oxidizing Mn₄CaO₅-cluster in photosystem II

Journal Article · Tue Jul 18 00:00:00 EDT 2017 · eLife

DOI:https://doi.org/10.7554/eLife.26933· OSTI ID:1408448

^[1]; Bommer, Martin ^[1]; Chatterjee, Ruchira ^[2]; Hussein, Rana ^[1]; Yano, Junko ^[2]; Dau, Holger ^[3]; Kern, Jan ^[2];

^[1];

^[1]

Humboldt Univ. of Berlin (Germany). Inst. of Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Freie Univ., Berlin (Germany)

In plants, algae and cyanobacteria, Photosystem II (PSII) catalyzes the light-driven splitting of water at a protein-bound Mn₄CaO₅-cluster, the water-oxidizing complex (WOC). In the photosynthetic organisms, the light-driven formation of the WOC from dissolved metal ions is a key process because it is essential in both initial activation and continuous repair of PSII. Structural information is required for understanding of this chaperone-free metal-cluster assembly. For the first time, we obtained a structure of PSII from Thermosynechococcus elongatus without the Mn₄CaO₅-cluster. Surprisingly, cluster-removal leaves the positions of all coordinating amino acid residues and most nearby water molecules largely unaffected, resulting in a pre-organized ligand shell for kinetically competent and error-free photo-assembly of the Mn₄CaO₅-cluster. First experiments initiating (i) partial disassembly and (ii) partial re-assembly after complete depletion of the Mn4CaO5-cluster agree with a specific bi-manganese cluster, likely a di-µ-oxo bridged pair of Mn(III) ions, as an assembly intermediate.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1408448

Journal Information:: eLife, Vol. 6; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Title: Structural insights into the light-driven auto-assembly process of the water-oxidizing Mn₄CaO₅-cluster in photosystem II