Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

Nelson, Robert S.; Peterson, Darren J.; Karp, Eric M.; Beckham, Gregg T.; Salvachua, Davinia Rodriguez

doi:10.3390/fermentation3010010

Title: Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

Journal Article · Wed Mar 01 00:00:00 EST 2017 · Fermentation

DOI:https://doi.org/10.3390/fermentation3010010· OSTI ID:1405918

Nelson, Robert S. ^[1]; Peterson, Darren J. ^[1]; Karp, Eric M. ^[1]; Beckham, Gregg T. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)

Here, volatile fatty acids (VFAs) can be readily produced from many anaerobic microbes and subsequently utilized as precursors to renewable biofuels and biochemicals. Megasphaera elsdenii represents a promising host for production of VFAs, butyric acid (BA) and hexanoic acid (HA). However, due to the toxicity of these acids, product removal via an extractive fermentation system is required to achieve high titers and productivities. Here, we examine multiple aspects of extractive separations to produce BA and HA from glucose and lignocellulosic hydrolysate with M. elsdenii. A mixture of oleyl alcohol and 10% (v/v) trioctylamine was selected as an extraction solvent due to its insignificant inhibitory effect on the bacteria. Batch extractive fermentations were conducted in the pH range of 5.0 to 6.5 to select the best cell growth rate and extraction efficiency combination. Subsequently, fed-batch pertractive fermentations were run over 230 h, demonstrating high BA and HA concentrations in the extracted fraction (57.2 g/L from ~190 g/L glucose) and productivity (0.26 g/L/h). To our knowledge, these are the highest combined acid titers and productivity values reported for M. elsdenii and bacterial mono-cultures from sugars. Lastly, the production of BA and HA (up to 17 g/L) from lignocellulosic sugars was demonstrated.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1405918

Report Number(s):: NREL/JA-5100-70383

Journal Information:: Fermentation, Vol. 3, Issue 1; ISSN 2311-5637

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Title: Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

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