Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy

Ocola, L. E.; Sampathkumar, V.; Kasthuri, N.; Winarski, R. P.

doi:10.1038/s41598-017-05690-6

Title: Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy

Journal Article · Wed Jul 19 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-05690-6· OSTI ID:1393905

^[1]; Sampathkumar, V. ^[2]; Kasthuri, N. ^[3]; Winarski, R. P. ^[1]

Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials
Univ. of Chicago, IL (United States). Dept. of Neurbiology
Univ. of Chicago, IL (United States). Dept. of Neurbiology; Argonne National Lab. (ANL), Argonne, IL (United States). Nanoscience and Technology Division

Here, we show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. This method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO₂ drying, or simple vacuum drying at 95°C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge (1s) absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; D16P00002

OSTI ID:: 1393905

Journal Information:: Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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