System and method for detecting components of a mixture including a valving scheme for competition assays

Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K.

Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Patent · Tue Sep 19 00:00:00 EDT 2017

OSTI ID:1393511

Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K.

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.

View Patent

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

DOE Contract Number:: NA0003525

Assignee:: National Technology & Engineering Solutions of Sandia, LLC

Patent Number(s):: 9,766,230

Application Number:: 15/616,740

OSTI ID:: 1393511

Resource Relation:: Patent File Date: 2017 Jun 07

Country of Publication:: United States

Language:: English

References (78)

Devices and Method For Using Centripetal Acceleration To Drive Fluid Movement In A Microfluidics System With On-Board Informatics Mian, Alec; Kieffer-Higgins, Stephen G.; Corey, George D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20010055812 US Patent Document 08/761063; 20010055812	patent-application	December 2001
Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device Lee, Jeong-gun; Lee, Beom-seok; Park, Jong-myeon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7758810 US Patent Document 7,758,810	patent	July 2010
Analytical methods and compositions Rao, Galla Chandra; Terstapper, Leon Wmm https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020164659 US Patent Application 10/017437; 20020164659	patent-application	November 2002
Microfluidic particle-analysis systems Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040072278 US Patent Application 10/405953; 20040072278	patent-application	April 2004
Method of separation employing magnetic particles and second medium Vorpahl, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5279936 US Patent Document 5,279,936	patent	January 1994
Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto Valencia, Ramoncito M.; Chen, YihFar; Hurt, Susan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7157049 US Patent Document 7,157,049	patent	January 2007
On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A. Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187 https://doi.org/10.1021/ac026239a	journal	March 2003
Multistation, Single Channel Analytical Photometer and Method of Use Anderson, Norman G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/3555284 US Patent Document 3,555,284	patent	January 1971
Centrifuge with removable core for scalable centrifugation Merino, Sandra Patricia; Dalessio, Steven J.; Rudy Otten, Robin Roy Louis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050215410 US Patent Application 11/129116; 20050215410	patent-application	September 2005
Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography Gusev, Isabelle; Huang, Xian; Horváth, Csaba Journal of Chromatography A, Vol. 855, Issue 1, p. 273-290 https://doi.org/10.1016/S0021-9673(99)00697-4	journal	September 1999
Method and apparatus for measuring blood constituent counts Wardlaw, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683579 US Patent Document 4,683,579	patent	July 1987
Microfluidic devices, systems, and methods for quantifying particles using centrifugal force Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9186668 US Patent Document 9,186,668	patent	November 2015
Diagnostic Analyzers With Pretreatment Carousels and Related Methods Ochranek, Brian L.; Arnquist, David C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140273241 US Patent Application 14/142474; 20140273241	patent-application	September 2014
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer Lim, Peniel; North, Robert; Vigh, Gyula ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859 https://doi.org/10.1002/elps.200600846	journal	June 2007
Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species Hefti, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030203504 US Patent Application 10/064392; 20030203504	patent-application	October 2003
A Method for Global Analysis of Complex Proteomes Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis Zuo, Xun; Speicher, David W. Analytical Biochemistry, Vol. 284, Issue 2 https://doi.org/10.1006/abio.2000.4714	journal	September 2000
Protecting agent Kange, Rickard; Inganas, Mats; Gustafsson, Magnus https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050186685 US Patent Application 11/038712; 20050186685	patent-application	August 2005
Method for detecting analytes in a sample Hinnah, Silke Christine; Lambru, Dagmar; Droge, Sonja https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090325186 US Patent Application 11/629107; 20090325186	patent-application	December 2009
Centrifugation Apparatus For Containers Of Biological Material Pedrazzini, Gianandrea https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110045958 US Patent Application 12/867345; 20110045958	patent-application	February 2011
Plastic microfluidics enabling two-dimensional protein separations in proteome analysis Lee, Cheng Sheng; DeVoe, Don https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020170825 US Patent Application 10/135386; 20020170825	patent-application	November 2002
Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González Biochimie, Vol. 86, Issue 4-5, p. 305-309 https://doi.org/10.1016/j.biochi.2004.03.009	journal	April 2004
Separation of solid and liquid components of mixtures Hunter, William M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4125375 US Patent Document 4,125,375	patent	November 1978
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8962346 US Patent Document 8,962,346	patent	February 2015
Compact disk based platform for separating and detecting immunomagnetic bead labeled cells Wo, Andrew M.; Chen, Chen-Lin; Chen, Ken-Chao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100151560 US Patent Application12/382844; 20100151560	patent-application	June 2010
Density-based cell detection system Kirakossian, Hrair https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100068754 US Patent Application 12/380877; 20100068754	patent	March 2010
Multichannel control in microfluidics Sassi, Alexander; Paulus, Aran https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020153251 US Patent Application 10/121378; 20020153251	patent-application	October 2002
Microparticle-based methods and systems and applications thereof Prober, James M.; Cui, Xiumin; Dam, Rudy J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050282220 US Patent Document 11/189080; 20050282220	patent-application	December 2005
Heterogeneous binding assay Baker, Terence Seward; Abbott, Stephen R.; Simpson, John G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4656143 US Patent Document 4,656,143	patent	April 1987
Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids Kellogg, Gregory John; Carvalho, Bruce L.; Sheppard, Jr., Norman F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7332326 US Patent Document 7,332,326	patent	February 2008
Microfluidics devices and methods for performing cell based assays Carvalho, Bruce L.; Sheppard, Norman F. JR.; Feakes, Christina https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020106786 US Patent Application 09/858558; 20020106786	patent-application	August 2002
Centrifugal Separation System Andersson, Lars https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090209402 US Patent Application 12/296982; 20090209402	patent-application	August 2009
Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates Amukele, Tim K.; Roday, Setu; Schramm, Vern L. Biochemistry, Vol. 44, Issue 11, p. 4416-4425 https://doi.org/10.1021/bi0474362	journal	March 2005
Monolithic microfluidic concentrators and mixers Frechet, Jean M. J.; Svec, Frantisek; Yu, Cong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6887384 US Patent Document 6,887,384	patent	May 2005
Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer Czeiger, David; Shaked, Gad; Eini, Hadar American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270 https://doi.org/10.1309/AJCP4RK2IHVKTTZV	journal	February 2011
A molecular approach to bioseparations: Protein–protein and protein–salt interactions Curtis, R. A.; Lue, L. Chemical Engineering Science, Vol. 61, Issue 3, p. 907-923 https://doi.org/10.1016/j.ces.2005.04.007	journal	February 2006
Functional integration of DNA purification and concentration into a real time micro-PCR chip Min, Junhong; Kim, Joon-Ho; Lee, Youngsun Lab on a Chip, Vol. 11, Issue 2, p. 259-265 https://doi.org/10.1039/C0LC00320D	journal	January 2011
Parallel Nanoliter Microfluidic Analysis System Andersson, Per; Jesson, Gerald; Kylberg, Gunnar Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030 https://doi.org/10.1021/ac061692y	journal	June 2007
Microfluidic particle-analysis systems Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7312085 US Patent Document 7,312,085	patent	December 2007
Detecting Single Stranded DNA with a Solid State Nanopore Fologea, Daniel; Gershow, Marc; Ledden, Bradley Nano Letters, Vol. 5, Issue 10, p. 1905-1909 https://doi.org/10.1021/nl051199m	journal	October 2005
Centrifugal hematology disposable Thomas, Bradley S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6153148 US Patent Document 6,153,148	patent	November 2000
Method for Separating Cells Woodside, Steven M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080108047 US Patent Application 11/940451; 20080108047	patent-application	May 2008
Toxin activity assays, devices, methods and systems therefor Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9304128 US Patent Document 9,304,128	patent	April 2016
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9304129 US Patent Document 9,304,129	patent	April 2016
Method For Isolating Nucleic Acids Comprising The Use Of Ethylene Glycol Multimers Finne, Erling https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090069554 US Patent Application 11/815967; 20090069554	patent-application	March 2009
Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device Ruijten, Henri M.; De Bree, Hans; Van Amsterdam, Peter H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4554071 US Patent Document 4,554,071	patent	November 1985
Assay system and method for conducting assays Andrevski, Zygmunt M.; Roach, William R.; Southgate, Peter D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5882903 US Patent Document 5,882,903	patent	March 1999
Method and Apparatus For Precise Transfer and Manipulation Of Fluids By Centrifugal and Or Capillary Forces Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090004059 US Patent Document 12/205965; 20090004059	patent-application	January 2009
Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228 https://doi.org/10.1097/ANA.0b013e31821b536a	journal	January 2011
Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886 https://doi.org/10.1021/ac050781s	journal	December 2005
Detection of Listeria monocytogenes and the toxin listeriolysin O in food Churchill, Robin L. T.; Lee, Hung; Hall, J. Christopher Journal of Microbiological Methods, Vol. 64, Issue 2, p. 141-170 https://doi.org/10.1016/j.mimet.2005.10.007	journal	February 2006
Method Of Detecting Analyte-Molecule Interactions Tolley, Luke; McCarroll, Matt https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080149484 US Patent Application 11/923079; 20080149484	patent-application	June 2008
Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions: Model and Experimental Validation Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul Analytical Chemistry, Vol. 73, Issue 3, p. 658-666 https://doi.org/10.1021/ac000495a	journal	February 2001
Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system Mian, Alec; Kieffer-Higgins, Stephen; Corey, George https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6319469 US Patent Document 6,319,469	patent	November 2001
Systems, devices, and methods for agglutination assays using sedimentation Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9244065 US Patent Document 9,244,065	patent	January 2016
Microfluidic devices and methods Jedrzejewski, Paul; Nock, Steffen; Wagner, Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030013203 US Patent Application 10/208381; 20030013203	patent-application	January 2003
Systems, Devices, and Methods For Agglutination Assays Using Sedimentation Schaff, Ulrich Y.; Sommer, Gregory Jon; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160061829 US Patent Application14/853660; 20160061829	patent-application	March 2016
Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc Lab on a Chip, Vol. 11, Issue 4, p. 723-726 https://doi.org/10.1039/C0LC00160K	journal	January 2011
Assay of blood or other biologic samples for target analytes Levine, Robert A.; Wardlaw, Stephen C.; Rodriguez, Rodolfo R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5635362 US Patent Document 5,635,362	patent	June 1997
Apparatus and method for carrying out analysis of samples Gordon, John Francis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5892577 US Patent Document 5,892,577	patent	April 1999
Integrated planar microfluidic bioanalytical systems Hawkins, Aaron R.; Peeni, Bridget; Barber, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060171654 US Patent Application 11/336646; 20060171654	patent-application	August 2006
Multi-parameter assays including analysis discs and methods relating thereto Gordon, John Francis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020151043 US Patent Application 10/121281; 20020151043	patent-application	October 2002
Toxin Activity Assays, Devices, Methods And Systems Therefor Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory Jon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160178619 US Patent Application 15/056870; 20160178619	patent-application	June 2016
Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device Das, Champak; Fan, Z. Hugh ELECTROPHORESIS, Vol. 27, Issue 18, p. 3619-3626 https://doi.org/10.1002/elps.200600013	journal	September 2006
Class characterization of circulating cancer cells isolated from body fluids and methods of use Wang, Zheng; Ts'o, Paul O. P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6960449 US Patent Document 6,960,449	patent	November 2005
Class characterization of circulating cancer cells isolated from body fluids and methods of use Wang, Zheng-Pin; TS'O, Paul O.P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020098535 US Patent Document 09/501179; 20020098535	patent-application	July 2002
Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang Lab on a Chip, Vol. 11, Issue 1, p. 70-78 https://doi.org/10.1039/C0LC00205D	journal	January 2011
Devices, systems, and methods for conducting sandwich assays using sedimentation Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8945914 US Patent Document 8,945,914	patent	February 2015
Diagnostic tests and kits for Clostridium difficile Valkirs, Gunars https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6503722 US Patent Document 6,503,722	patent	January 2003
Apparatus and method for measuring white blood cell and platelet concentrations in blood Wardlaw, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4156570 US Patent Document 4,156,570	patent	May 1979
DNA purification and isolation using magnetic particles Hawkins, Trevor https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5705628 US Patent Document 5,705,628	patent	January 1998
System and method for detecting components of a mixture including tooth elements for alignment Sommer, Gregory J.; Schaff, Ulrich Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9500579 US Patent Document 9,500,579	patent	November 2016
Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8337775 US Patent Document 8,337,775	patent	December 2012
Separation Device Froman, Gunnar; Haneskog, Lars; Johansson, Bjorn A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100120596 US Patent Application12/597925; 20100120596	patent-application	May 2010
Loading Disk for Photometric Analyzer of Rotary Cuvette Type Maddox, Wayne L.; Coleman, Robert L.; Shults III, Wilbur D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/3744974 US Patent Document 3,744,974	patent	July 1973
Method for separating the cellular components of blood samples Smith, Ward C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4844818 US Patent Document 4,844,818	patent	July 1989
Method for separating cells Woodside, Steven M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030124719 US Patent Application 10/260560; 2003012471	patent-application	July 2003
Multi-parameter assays including analysis discs and methods relating thereto Gordon, John Francis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7033747 US Patent Document 7,033,747	patent	April 2006
Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force Schaff, Ulrich Y.; Sommer, Gregory Jon; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20150360225 US Patent Application 14/836665; 20150360225	patent-application	December 2015

Similar Records

System and method for detecting components of a mixture including a valving scheme for competition assays

Patent · Tue Jul 11 00:00:00 EDT 2017 · OSTI ID:1393511

Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K.

Broad base biological assay using liquid based detection assays

Technical Report · Tue Oct 31 00:00:00 EST 2000 · OSTI ID:1393511

Milanovich, F; Albala, J; Colston, B; +2 more

125I anti-immunoglobulin binding assay for the detection and characterization of anti-platelet antibodies

Journal Article · Mon Dec 01 00:00:00 EST 1980 · Hum. Immunol.; (United States) · OSTI ID:1393511

Kirkley, J; Fabre, J W

Related Subjects

42 ENGINEERING
59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION

Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Citation Formats

References (78)

Similar Records

Related Subjects