Biological iron-sulfur storage in a thioferrate-protein nanoparticle

Vaccaro, Brian J.; Clarkson, Sonya M.; Holden, James F.; Lee, Dong-Woo; Wu, Chang-Hao; Poole II, Farris L.; Cotelesage, Julien J.  H.; Hackett, Mark J.; Mohebbi, Sahel; Sun, Jingchuan; Li, Huilin; Johnson, Michael K.; George, Graham N.; Adams, Michael W.  W.

doi:10.1038/ncomms16110

Title: Biological iron-sulfur storage in a thioferrate-protein nanoparticle

Journal Article · Thu Jul 20 00:00:00 EDT 2017 · Nature Communications

DOI:https://doi.org/10.1038/ncomms16110· OSTI ID:1393138

Vaccaro, Brian J. ^[1]; Clarkson, Sonya M. ^[2];

^[2]; Lee, Dong-Woo ^[2]; Wu, Chang-Hao ^[2]; Poole II, Farris L. ^[2]; Cotelesage, Julien J. H. ^[3]; Hackett, Mark J. ^[3]; Mohebbi, Sahel ^[4]; Sun, Jingchuan ^[5]; Li, Huilin ^[5]; Johnson, Michael K. ^[4]; George, Graham N. ^[3]; Adams, Michael W. W. ^[2]

Univ. of Georgia, Athens, GA (United States). Dept. of Chemistry, Dept. of Biochemistry and Molecular Biology
Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
Univ. of Saskatchewan, Saskatoon, SK (Canada). Dept. of Geological Sciences and Chemistry
Univ. of Georgia, Athens, GA (United States). Dept. of Chemistry
Center for Epigenetics, Grand Rapids, MI (United States). Van Andel Research Inst.

Iron–sulfur clusters are ubiquitous in biology and function in electron transfer and catalysis. We assembled them from iron and cysteine sulfur on protein scaffolds. Iron is typically stored as iron oxyhydroxide, ferrihydrite, encapsulated in 12 nm shells of ferritin, which buffers cellular iron availability. We have characterized IssA, a protein that stores iron and sulfur as thioferrate, an inorganic anionic polymer previously unknown in biology. IssA forms nanoparticles reaching 300 nm in diameter and is the largest natural metalloprotein complex known. It is a member of a widely distributed protein family that includes nitrogenase maturation factors, NifB and NifX. IssA nanoparticles are visible by electron microscopy as electron-dense bodies in the cytoplasm. Purified nanoparticles appear to be generated from 20 nm units containing B 6,400 Fe atoms and B 170 IssA monomers. In support of roles in both iron–sulfur storage and cluster biosynthesis, IssA reconstitutes the [4Fe-4S] cluster in ferredoxin in vitro.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: FG05-95ER20175; GM62524

OSTI ID:: 1393138

Journal Information:: Nature Communications, Vol. 8; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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