A synthetic biochemistry platform for cell free production of monoterpenes from glucose

Korman, Tyler P.; Opgenorth, Paul H.; Bowie, James U.

doi:10.1038/ncomms15526

Title: A synthetic biochemistry platform for cell free production of monoterpenes from glucose

Journal Article · Wed May 24 00:00:00 EDT 2017 · Nature Communications

DOI:https://doi.org/10.1038/ncomms15526· OSTI ID:1389298

Korman, Tyler P. ^[1]; Opgenorth, Paul H. ^[1]; Bowie, James U. ^[1]

Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry

Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells. We design a system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes. Different monoterpenes are produced in our system by changing the terpene synthase enzyme. The system is stable for the production of limonene, pinene and sabinene, and can operate continuously for at least 5 days from a single addition of glucose. We also obtain conversion yields 495% and titres 415 g l^-1. The titres are an order of magnitude over cellular toxicity limits and thus difficult to achieve using cell-based systems. Overall, these results highlight the potential of synthetic biochemistry approaches for producing bio-based chemicals.

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Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-02ER63421; AR0000556

OSTI ID:: 1389298

Journal Information:: Nature Communications, Vol. 8; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 134 works

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