Transcriptomic Analyses Elucidate Adaptive Differences of Closely Related Strains of Pseudomonas aeruginosa in Fuel

Gunasekera, Thusitha S.; Bowen, Loryn L.; Zhou, Carol E.; Howard-Byerly, Susan C.; Foley, William S.; Striebich, Richard C.; Dugan, Larry C.; Ruiz, Oscar N.

doi:10.1128/AEM.03249-16

Title: Transcriptomic Analyses Elucidate Adaptive Differences of Closely Related Strains of Pseudomonas aeruginosa in Fuel

Journal Article · Fri Mar 17 00:00:00 EDT 2017 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.03249-16· OSTI ID:1366911

Gunasekera, Thusitha S. ^[1]; Bowen, Loryn L. ^[1]; Zhou, Carol E. ^[2]; Howard-Byerly, Susan C. ^[1]; Foley, William S. ^[3]; Striebich, Richard C. ^[1]; Dugan, Larry C. ^[2]; Ruiz, Oscar N. ^[4]

Univ. of Dayton, Dayton, OH (United States). Univ. of Dayton Research Inst.
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Air Force Research Lab. (AFRL), Wright-Patterson AFB, OH (United States). Fuels and Energy Branch, Aerospace Systems Directorate
Air Force Research Lab. (AFRL), Wright-Patterson AFB, OH (United States). Fuels and Energy Branch, Aerospace Systems Directorate

Pseudomonas aeruginosacan utilize hydrocarbons, but different strains have various degrees of adaptation despite their highly conserved genome.P. aeruginosaATCC 33988 is highly adapted to hydrocarbons, whileP. aeruginosastrain PAO1, a human pathogen, is less adapted and degrades jet fuel at a lower rate than does ATCC 33988. We investigated fuel-specific transcriptomic differences between these strains in order to ascertain the underlying mechanisms utilized by the adapted strain to proliferate in fuel. During growth in fuel, the genes related to alkane degradation, heat shock response, membrane proteins, efflux pumps, and several novel genes were upregulated in ATCC 33988. Overexpression ofalkgenes in PAO1 provided some improvement in growth, but it was not as robust as that of ATCC 33988, suggesting the role of other genes in adaptation. Expression of the function unknown gene PA5359 from ATCC 33988 in PAO1 increased the growth in fuel. Bioinformatic analysis revealed that PA5359 is a predicted lipoprotein with a conserved Yx(FWY)xxD motif, which is shared among bacterial adhesins. Overexpression of the putative resistance-nodulation-division (RND) efflux pump PA3521 to PA3523 increased the growth of the ATCC 33988 strain, suggesting a possible role in fuel tolerance. Interestingly, the PAO1 strain cannot utilizen-C₈andn-C₁₀. The expression of green fluorescent protein (GFP) under the control ofalkBpromoters confirmed thatalkgene promoter polymorphism affects the expression ofalkgenes. Promoter fusion assays further confirmed that the regulation ofalkgenes was different in the two strains. Protein sequence analysis showed low amino acid differences for many of the upregulated genes, further supporting transcriptional control as the main mechanism for enhanced adaptation. Lastly, these results support that specific signal transduction, gene regulation, and coordination of multiple biological responses are required to improve the survival, growth, and metabolism of fuel in adapted strains. This study provides new insight into the mechanistic differences between strains and helpful information that may be applied in the improvement of bacterial strains for resistance to biotic and abiotic factors encountered during bioremediation and industrial biotechnological processes.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1366911

Report Number(s):: LLNL-JRNL-701003

Journal Information:: Applied and Environmental Microbiology, Vol. 83, Issue 10; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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References (73)

The Accessory Genome of Pseudomonas aeruginosa Kung, Vanderlene L.; Ozer, Egon A.; Hauser, Alan R. Microbiology and Molecular Biology Reviews, Vol. 74, Issue 4 https://doi.org/10.1128/MMBR.00027-10	journal	December 2010
Environmental and clinical isolates of Pseudomonas aeruginosa show pathogenic and biodegradative properties irrespective of their origin Alonso, Ana; Rojo, Fernando; Martinez, Jose L. Environmental Microbiology, Vol. 1, Issue 5 https://doi.org/10.1046/j.1462-2920.1999.00052.x	journal	September 1999
Pseudomonas aeruginosa clinical and environmental isolates constitute a single population with high phenotypic diversity Grosso-Becerra, María-Victoria; Santos-Medellín, Christian; González-Valdez, Abigail BMC Genomics, Vol. 15, Issue 1 https://doi.org/10.1186/1471-2164-15-318	journal	January 2014
What It Takes to Be a Pseudomonas aeruginosa? The Core Genome of the Opportunistic Pathogen Updated Valot, Benoît; Guyeux, Christophe; Rolland, Julien Yves PLOS ONE, Vol. 10, Issue 5 https://doi.org/10.1371/journal.pone.0126468	journal	May 2015
Transcriptional Profiling Suggests that Multiple Metabolic Adaptations are Required for Effective Proliferation of Pseudomonas aeruginosa in Jet Fuel Gunasekera, Thusitha S.; Striebich, Richard C.; Mueller, Susan S. Environmental Science & Technology, Vol. 47, Issue 23 https://doi.org/10.1021/es403163k	journal	November 2013
Characterization of the F-76 diesel and Jet-A aviation fuel hydrocarbon degradation profiles of Pseudomonas aeruginosa and Marinobacter hydrocarbonoclasticus Striebich, Richard C.; Smart, Caitlin E.; Gunasekera, Thusitha S. International Biodeterioration & Biodegradation, Vol. 93 https://doi.org/10.1016/j.ibiod.2014.04.024	journal	September 2014
Draft Genome Sequence of Pseudomonas aeruginosa ATCC 33988, a Bacterium Highly Adapted to Fuel-Polluted Environments Brown, L. M.; Gunasekera, T. S.; Ruiz, O. N. Genome Announcements, Vol. 2, Issue 6 https://doi.org/10.1128/genomeA.01113-14	journal	November 2014
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen Stover, C. K.; Pham, X. Q.; Erwin, A. L. Nature, Vol. 406, Issue 6799, p. 959-964 https://doi.org/10.1038/35023079	journal	August 2000
Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa Smits, Theo H. M.; Witholt, Bernard; van Beilen, Jan B. Antonie van Leeuwenhoek, Vol. 84, Issue 3, p. 193-200 https://doi.org/10.1023/A:1026000622765	journal	January 2003
Expression of the Pseudomonas putida OCT Plasmid Alkane Degradation Pathway Is Modulated by Two Different Global Control Signals: Evidence from Continuous Cultures Dinamarca, M. Alejandro; Aranda-Olmedo, Isabel; Puyet, Antonio Journal of Bacteriology, Vol. 185, Issue 16 https://doi.org/10.1128/JB.185.16.4772-4778.2003	journal	August 2003
The Pseudomonas putida Crc global regulator is an RNA binding protein that inhibits translation of the AlkS transcriptional regulator Moreno, Renata; Ruiz-Manzano, Ana; Yuste, Luis Molecular Microbiology, Vol. 64, Issue 3 https://doi.org/10.1111/j.1365-2958.2007.05685.x	journal	May 2007
The Crc and Hfq proteins of P seudomonas putida cooperate in catabolite repression and formation of ribonucleic acid complexes with specific target motifs: Role of Crc and Hfq in catabolite repression Moreno, Renata; Hernández-Arranz, Sofía; La Rosa, Ruggero Environmental Microbiology, Vol. 17, Issue 1 https://doi.org/10.1111/1462-2920.12499	journal	June 2014
Regulation of Hfq by the RNA CrcZ in Pseudomonas aeruginosa Carbon Catabolite Repression Sonnleitner, Elisabeth; Bläsi, Udo PLoS Genetics, Vol. 10, Issue 6 https://doi.org/10.1371/journal.pgen.1004440	journal	June 2014
Pathogenic adaptation of intracellular bacteria by rewiring a cis-regulatory input function Osborne, S. E.; Walthers, D.; Tomljenovic, A. M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 10 https://doi.org/10.1073/pnas.0811669106	journal	February 2009
A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence de Greeff, Astrid; Buys, Herma; Wells, Jerry M. BMC Microbiology, Vol. 14, Issue 1 https://doi.org/10.1186/s12866-014-0264-9	journal	November 2014
RNA-Seq and Microarrays Analyses Reveal Global Differential Transcriptomes of Mesorhizobium huakuii 7653R between Bacteroids and Free-Living Cells Peng, Jieli; Hao, Baohai; Liu, Liu PLoS ONE, Vol. 9, Issue 4 https://doi.org/10.1371/journal.pone.0093626	journal	April 2014
Genome-Wide Transcriptional Responses of Escherichia coli K-12 to Continuous Osmotic and Heat Stresses Gunasekera, T. S.; Csonka, L. N.; Paliy, O. Journal of Bacteriology, Vol. 190, Issue 10 https://doi.org/10.1128/JB.01990-07	journal	March 2008
How fast-growing bacteria robustly tune their ribosome concentration to approximate growth-rate maximization Bosdriesz, Evert; Molenaar, Douwe; Teusink, Bas FEBS Journal, Vol. 282, Issue 10 https://doi.org/10.1111/febs.13258	journal	March 2015
Differential Expression of the Components of the Two Alkane Hydroxylases from Pseudomonas aeruginosa Marin, M. M.; Yuste, L.; Rojo, F. Journal of Bacteriology, Vol. 185, Issue 10 https://doi.org/10.1128/JB.185.10.3232-3237.2003	journal	May 2003
The Lon protease homologue LonA, not LonC, contributes to the stress tolerance and biofilm formation of Actinobacillus pleuropneumoniae Xie, Fang; Li, Gang; Zhang, Yanhe Microbial Pathogenesis, Vol. 93 https://doi.org/10.1016/j.micpath.2016.01.009	journal	April 2016
Transcriptional Tradeoff between Metabolic and Stress-response Programs in Pseudomonas putida KT2440 Cells Exposed to Toluene Domínguez-Cuevas, Patricia; González-Pastor, José-Eduardo; Marqués, Silvia Journal of Biological Chemistry, Vol. 281, Issue 17 https://doi.org/10.1074/jbc.M509848200	journal	February 2006
Mechanisms of solvent resistance mediated by interplay of cellular factors in Pseudomonas putida Ramos, Juan-Luis; Sol Cuenca, Maria; Molina-Santiago, Carlos FEMS Microbiology Reviews, Vol. 39, Issue 4 https://doi.org/10.1093/femsre/fuv006	journal	April 2015
A Toluene-tolerant Mutant of Pseudomonas aeruginosa Lacking the Outer Membrane Protein F Li, Lina; Komatsu, Tetsushi; Inoue, Akira Bioscience, Biotechnology, and Biochemistry, Vol. 59, Issue 12 https://doi.org/10.1271/bbb.59.2358	journal	January 1995
The Crystal Structure of OprG from Pseudomonas aeruginosa, a Potential Channel for Transport of Hydrophobic Molecules across the Outer Membrane Touw, Debra S.; Patel, Dimki R.; van den Berg, Bert PLoS ONE, Vol. 5, Issue 11 https://doi.org/10.1371/journal.pone.0015016	journal	November 2010
Transcriptional control of the main aromatic hydrocarbon efflux pump in Pseudomonas: Solvent tolerance traits Fillet, Sandy; Daniels, Craig; Pini, Cecilia Environmental Microbiology Reports, Vol. 4, Issue 2 https://doi.org/10.1111/j.1758-2229.2011.00255.x	journal	April 2011
Engineering microbial biofuel tolerance and export using efflux pumps Dunlop, Mary J.; Dossani, Zain Y.; Szmidt, Heather L. Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.21	journal	January 2011
Efflux systems in bacteria and their metabolic engineering applications Jones, Christopher M.; Hernández Lozada, Néstor J.; Pfleger, Brian F. Applied Microbiology and Biotechnology, Vol. 99, Issue 22 https://doi.org/10.1007/s00253-015-6963-9	journal	September 2015
Identification and Characterization of Inhibitors of Multidrug Resistance Efflux Pumps in Pseudomonas aeruginosa: Novel Agents for Combination Therapy Lomovskaya, Olga; Warren, Mark S.; Lee, Angela Antimicrobial Agents and Chemotherapy, Vol. 45, Issue 1 https://doi.org/10.1128/AAC.45.1.105-116.2001	journal	January 2001
Inhibitors of Multidrug Resistant Efflux Systems in Bacteria Zechini, Barbara; Versace, Ilaria Recent Patents on Anti-Infective Drug Discovery, Vol. 4, Issue 1 https://doi.org/10.2174/157489109787236256	journal	January 2009
Sequence-Based Prediction of Protein–Carbohydrate Binding Sites Using Support Vector Machines Taherzadeh, Ghazaleh; Zhou, Yaoqi; Liew, Alan Wee-Chung Journal of Chemical Information and Modeling, Vol. 56, Issue 10 https://doi.org/10.1021/acs.jcim.6b00320	journal	September 2016
A single-nucleotide mutation in the −10 promoter region inactivates the narK2X promoter in Mycobacterium bovis and Mycobacterium bovis BCG and has an application in diagnosis Chauhan, Santosh; Singh, Alka; Tyagi, Jaya Sivaswami FEMS Microbiology Letters, Vol. 303, Issue 2 https://doi.org/10.1111/j.1574-6968.2009.01876.x	journal	February 2010
Degradation of alkanes by bacteria Rojo, Fernando Environmental Microbiology, Vol. 11, Issue 10 https://doi.org/10.1111/j.1462-2920.2009.01948.x	journal	October 2009
Prodigal: prokaryotic gene recognition and translation initiation site identification Hyatt, Doug; Chen, Gwo-Liang; LoCascio, Philip F. BMC Bioinformatics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2105-11-119	journal	March 2010
Identifying bacterial genes and endosymbiont DNA with Glimmer Delcher, Arthur L.; Bratke, Kirsten A.; Powers, Edwin C. Bioinformatics, Vol. 23, Issue 6 https://doi.org/10.1093/bioinformatics/btm009	journal	January 2007
GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions Besemer, J. Nucleic Acids Research, Vol. 29, Issue 12 https://doi.org/10.1093/nar/29.12.2607	journal	June 2001
Conserved regulatory elements of the promoter sequence of the gene rpoH of enteric bacteria Ramirez-Santos, J. Nucleic Acids Research, Vol. 29, Issue 2 https://doi.org/10.1093/nar/29.2.380	journal	January 2001
Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa Ulrey, Robert K.; Barksdale, Stephanie M.; Zhou, Weidong BMC Complementary and Alternative Medicine, Vol. 14, Issue 1 https://doi.org/10.1186/1472-6882-14-499	journal	December 2014
The carbohydrate-active enzymes database (CAZy) in 2013 Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1178	journal	November 2013
Effect of Conventional and Alternative Fuels on a Marine Bacterial Community and the Significance to Bioremediation Ruiz, Oscar N.; Brown, Lisa M.; Striebich, Richard C. Energy & Fuels, Vol. 30, Issue 1 https://doi.org/10.1021/acs.energyfuels.5b02439	journal	December 2015
The RAST Server: Rapid Annotations using Subsystems Technology Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A. BMC Genomics, Vol. 9, Issue 1, Article No. 75 https://doi.org/10.1186/1471-2164-9-75	journal	January 2008
Protein Sequence Annotation Tool (PSAT): a centralized web-based meta-server for high-throughput sequence annotations Leung, Elo; Huang, Amy; Cadag, Eithon BMC Bioinformatics, Vol. 17, Issue 1 https://doi.org/10.1186/s12859-016-0887-y	journal	January 2016
EFICAz2.5: application of a high-precision enzyme function predictor to 396 proteomes Kumar, Narendra; Skolnick, Jeffrey Bioinformatics, Vol. 28, Issue 20 https://doi.org/10.1093/bioinformatics/bts510	journal	August 2012
KEGG as a reference resource for gene and protein annotation Kanehisa, Minoru; Sato, Yoko; Kawashima, Masayuki Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1070	journal	October 2015
The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databases Caspi, Ron; Billington, Richard; Ferrer, Luciana Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1164	journal	November 2015
BRENDA in 2015: exciting developments in its 25th year of existence Chang, Antje; Schomburg, Ida; Placzek, Sandra Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1068	journal	November 2014
STRING v10: protein–protein interaction networks, integrated over the tree of life Szklarczyk, Damian; Franceschini, Andrea; Wyder, Stefan Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1003	journal	October 2014
InterProScan 5: genome-scale protein function classification Jones, P.; Binns, D.; Chang, H. -Y. Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu031	journal	January 2014
SignalP 4.0: discriminating signal peptides from transmembrane regions Petersen, Thomas Nordahl; Brunak, Søren; von Heijne, Gunnar Nature Methods, Vol. 8, Issue 10 https://doi.org/10.1038/nmeth.1701	journal	September 2011
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen Krogh, Anders; Larsson, Björn; von Heijne, Gunnar Journal of Molecular Biology, Vol. 305, Issue 3 https://doi.org/10.1006/jmbi.2000.4315	journal	January 2001
Pseudomonas Genome Database: improved comparative analysis and population genomics capability for Pseudomonas genomes Winsor, G. L.; Lam, D. K. W.; Fleming, L. Nucleic Acids Research, Vol. 39, Issue Database https://doi.org/10.1093/nar/gkq869	journal	October 2010
TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels Ren, Q.; Chen, K.; Paulsen, I. T. Nucleic Acids Research, Vol. 35, Issue Database https://doi.org/10.1093/nar/gkl925	journal	January 2007
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega Sievers, Fabian; Wilm, Andreas; Dineen, David Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.75	journal	January 2011
CDD: NCBI's conserved domain database Marchler-Bauer, Aron; Derbyshire, Myra K.; Gonzales, Noreen R. Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1221	journal	November 2014
Expression stability of six housekeeping genes: a proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR Savli, Hakan; Karadenizli, Aynur; Kolayli, Fetiye Journal of Medical Microbiology, Vol. 52, Issue 5 https://doi.org/10.1099/jmm.0.05132-0	journal	May 2003
Construction of Mobilizable Mini-Tn 7 Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy Promoter lux Reporter Analysis Damron, F. Heath; McKenney, Elizabeth S.; Barbier, Mariette Applied and Environmental Microbiology, Vol. 79, Issue 13 https://doi.org/10.1128/AEM.00640-13	journal	April 2013
ChIP-Seq and RNA-Seq Reveal an AmrZ-Mediated Mechanism for Cyclic di-GMP Synthesis and Biofilm Development by Pseudomonas aeruginosa Jones, Christopher J.; Newsom, David; Kelly, Benjamin PLoS Pathogens, Vol. 10, Issue 3 https://doi.org/10.1371/journal.ppat.1003984	journal	March 2014
Transcriptome Analysis of the Response of Pseudomonas aeruginosa to Hydrogen Peroxide Palma, Marco; DeLuca, Darrow; Worgall, Stefan Journal of Bacteriology, Vol. 186, Issue 1 https://doi.org/10.1128/JB.186.1.248-252.2004	journal	January 2004
Host and invader impact of transfer of the clc genomic island into Pseudomonas aeruginosa PAO1 Gaillard, M.; Pernet, N.; Vogne, C. Proceedings of the National Academy of Sciences, Vol. 105, Issue 19 https://doi.org/10.1073/pnas.0801269105	journal	April 2008
Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptome analysis Folsom, James P.; Richards, Lee; Pitts, Betsey BMC Microbiology, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2180-10-294	journal	January 2010
Parallel Evolution in Pseudomonas aeruginosa over 39,000 Generations In Vivo Huse, Holly K.; Kwon, Taejoon; Zlosnik, James E. A. mBio, Vol. 1, Issue 4 https://doi.org/10.1128/mBio.00199-10	journal	September 2010
Azithromycin Inhibits Expression of the GacA-Dependent Small RNAs RsmY and RsmZ in Pseudomonas aeruginosa Pérez-Martínez, Isabel; Haas, Dieter Antimicrobial Agents and Chemotherapy, Vol. 55, Issue 7 https://doi.org/10.1128/AAC.01801-10	journal	May 2011
Ser/Thr/Tyr phosphoproteome analysis of pathogenic and non-pathogenic Pseudomonas species Ravichandran, Ayshwarya; Sugiyama, Naoyuki; Tomita, Masaru PROTEOMICS, Vol. 9, Issue 10 https://doi.org/10.1002/pmic.200800655	journal	May 2009
Identification of Five Structurally Unrelated Quorum-Sensing Inhibitors of Pseudomonas aeruginosa from a Natural-Derivative Database Tan, Sean Yang-Yi; Chua, Song-Lin; Chen, Yicai Antimicrobial Agents and Chemotherapy, Vol. 57, Issue 11 https://doi.org/10.1128/AAC.00955-13	journal	September 2013
Responses of Pseudomonas aeruginosa to low oxygen indicate that growth in the cystic fibrosis lung is by aerobic respiration Alvarez-Ortega, Carolina; Harwood, Caroline S. Molecular Microbiology, Vol. 65, Issue 1 https://doi.org/10.1111/j.1365-2958.2007.05772.x	journal	July 2007
Use of cell wall stress to characterize σ22 (AlgT/U) activation by regulated proteolysis and its regulon in Pseudomonas aeruginosa: σ22 regulon in P. aeruginosa Wood, Lynn F.; Ohman, Dennis E. Molecular Microbiology, Vol. 72, Issue 1 https://doi.org/10.1111/j.1365-2958.2009.06635.x	journal	March 2009
The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa Gooderham, W. James; Gellatly, Shaan L.; Sanschagrin, François Microbiology, Vol. 155, Issue 3 https://doi.org/10.1099/mic.0.024554-0	journal	March 2009
Dodecins: A Family of Lumichrome Binding Proteins Grininger, Martin; Zeth, Kornelius; Oesterhelt, Dieter Journal of Molecular Biology, Vol. 357, Issue 3 https://doi.org/10.1016/j.jmb.2005.12.072	journal	March 2006
Mechanistic Characterization of N -Formimino- l -glutamate Iminohydrolase from Pseudomonas aeruginosa ^† Martí-Arbona, Ricardo; Raushel, Frank M. Biochemistry, Vol. 45, Issue 48 https://doi.org/10.1021/bi061673i	journal	December 2006
Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa Skindersoe, Mette E.; Alhede, Morten; Phipps, Richard Antimicrobial Agents and Chemotherapy, Vol. 52, Issue 10 https://doi.org/10.1128/AAC.01230-07	journal	July 2008
Epidemiological investigation of Pseudomonas aeruginosa isolates from a six-year-long hospital outbreak using high-throughput whole genome sequencing Snyder, L. A.; Loman, N. J.; Faraj, L. A. Eurosurveillance, Vol. 18, Issue 42 https://doi.org/10.2807/1560-7917.ES2013.18.42.20611	journal	October 2013
Intraclonal diversity of the Pseudomonas aeruginosa cystic fibrosis airway isolates TBCF10839 and TBCF121838: distinct signatures of transcriptome, proteome, metabolome, adherence and pathogenicity despite an almost identical genome sequence: Genome and phenome diversity of P. aeruginosa clone TB Klockgether, Jens; Miethke, Norbert; Kubesch, Peter Environmental Microbiology, Vol. 15, Issue 1 https://doi.org/10.1111/j.1462-2920.2012.02842.x	journal	August 2012
A Temporal Examination of the Planktonic and Biofilm Proteome of Whole Cell Pseudomonas aeruginosa PAO1 Using Quantitative Mass Spectrometry Park, Amber J.; Murphy, Kathleen; Krieger, Jonathan R. Molecular & Cellular Proteomics, Vol. 13, Issue 4 https://doi.org/10.1074/mcp.M113.033985	journal	February 2014
Comparative microarray analysis reveals that the core biofilm-associated transcriptome of Pseudomonas aeruginosa comprises relatively few genes: Biofilm-associated gene expression in P. aeruginosa Patell, Sanaya; Gu, Muxin; Davenport, Peter Environmental Microbiology Reports, Vol. 2, Issue 3 https://doi.org/10.1111/j.1758-2229.2010.00158.x	journal	March 2010

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GFP
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genomics
jet fuel
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stress response
transcriptome

Title: Transcriptomic Analyses Elucidate Adaptive Differences of Closely Related Strains of Pseudomonas aeruginosa in Fuel

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