SpyB, a small heme-binding protein, affects the composition of the cell wall in Streptococcus pyogenes

Edgar, Rebecca J.; Chen, Jing; Kant, Sashi; Rechkina, Elena; Rush, Jeffrey S.; Forsberg, Lennart S.; Jaehrig, Bernhard; Azadi, Parastoo; Tchesnokova, Veronika; Sokurenko, Evgeni V.; Zhu, Haining; Korotkov, Konstantin V.; Pancholi, Vijay; Korotkova, Natalia

doi:10.3389/fcimb.2016.00126

Title: SpyB, a small heme-binding protein, affects the composition of the cell wall in Streptococcus pyogenes

Journal Article · Thu Oct 13 00:00:00 EDT 2016 · Frontiers in Cellular and Infection Microbiology

DOI:https://doi.org/10.3389/fcimb.2016.00126· OSTI ID:1365842

Edgar, Rebecca J. ^[1]; Chen, Jing ^[1]; Kant, Sashi ^[2]; Rechkina, Elena ^[3]; Rush, Jeffrey S. ^[1]; Forsberg, Lennart S. ^[4]; Jaehrig, Bernhard ^[4]; Azadi, Parastoo ^[4]; Tchesnokova, Veronika ^[3]; Sokurenko, Evgeni V. ^[3]; Zhu, Haining ^[1]; Korotkov, Konstantin V. ^[1]; Pancholi, Vijay ^[2]; Korotkova, Natalia ^[1]

Univ. of Kentucky, Lexington, KY (United States)
The Ohio State Univ., Columbus, OH (United States)
Univ. of Washington, Seattle, WA (United States)
Univ. of Georgia, Athens, GA (United States)

Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrhamnose backbone with immunodominant N-acetylglucosamine side-chains. All GAS genomes contain the spyBA operon, which encodes a 35-amino-acid membrane protein SpyB, and a membrane-bound C₃-like ADP-ribosyltransferase SpyA. In this study, we addressed the function of SpyB in GAS. Phenotypic analysis of a spyB deletion mutant revealed increased bacterial aggregation, and reduced sensitivity to β-lactams of the cephalosporin class and peptidoglycan hydrolase PlyC. Glycosyl composition analysis of cell wall isolated from the spyB mutant suggested an altered carbohydrate structure compared with the wild-type strain. Furthermore, we found that SpyB associates with heme and protoporphyrin IX. Heme binding induces SpyB dimerization, which involves disulfide bond formation between the subunits. Furthermore, our data suggest the possibility that SpyB activity is regulated by heme.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1365842

Journal Information:: Frontiers in Cellular and Infection Microbiology, Vol. 6; ISSN 2235-2988

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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SpyA
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ADP-ribosyltransferase
Group A carbohydrate
Streptococcus pyogenes
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Title: SpyB, a small heme-binding protein, affects the composition of the cell wall in Streptococcus pyogenes

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