Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Le, Rosemary K.; Wells Jr., Tyrone; Das, Parthapratim; Meng, Xianzhi; Stoklosa, Ryan J.; Bhalla, Aditya; Hodge, David B.; Yuan, Joshua S.; Ragauskas, Arthur J.

doi:10.1039/c6ra28033a

Title: Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Journal Article · Fri Jan 13 00:00:00 EST 2017 · RSC Advances

DOI:https://doi.org/10.1039/c6ra28033a· OSTI ID:1356963

Le, Rosemary K. ^[1]; Wells Jr., Tyrone ^[1]; Das, Parthapratim ^[1]; Meng, Xianzhi ^[1]; Stoklosa, Ryan J. ^[2]; Bhalla, Aditya ^[3]; Hodge, David B. ^[4]; Yuan, Joshua S. ^[5]; Ragauskas, Arthur J. ^[6]

Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division
Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science and Great Lakes Bioenergy Research Center
Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center and Department of Biochemistry
Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science, Great Lakes Bioenergy Research Center and Department of Biosystems & Agricultural Engineering
Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub, Department of Plant Pathology and Microbiology
Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering and Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division

We present the bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719^T . Here we show using ³¹P-NMR, HPAECPAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (70 300 g mol1 ) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L1 and accumulated 42.1% in oils based on cell dry weight after 48 h.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; EE0006112

OSTI ID:: 1356963

Journal Information:: RSC Advances, Vol. 7, Issue 7; ISSN 2046-2069

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 40 works

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