Detection of protease and protease activity using a single nanocrescent SERS probe
Abstract
This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.
- Inventors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1351818
- Patent Number(s):
- 9,145,575
- Application Number:
- 13/752,069
- Assignee:
- The Regents of the University of California (Oakland, CA)
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Jan 28
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES
Citation Formats
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. Detection of protease and protease activity using a single nanocrescent SERS probe. United States: N. p., 2015.
Web.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., & Chen, Fanqing Frank. Detection of protease and protease activity using a single nanocrescent SERS probe. United States.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. 2015.
"Detection of protease and protease activity using a single nanocrescent SERS probe". United States. https://www.osti.gov/servlets/purl/1351818.
@article{osti_1351818,
title = {Detection of protease and protease activity using a single nanocrescent SERS probe},
author = {Liu, Gang L. and Ellman, Jonathan A. and Lee, Luke P. and Chen, Fanqing Frank},
abstractNote = {This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.},
doi = {},
url = {https://www.osti.gov/biblio/1351818},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 29 00:00:00 EDT 2015},
month = {Tue Sep 29 00:00:00 EDT 2015}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Raman-based system for DNA sequencing-mapping and other separations
patent, April 1994
- Vo-Dinh, Tuan
- US Patent Document 5,306,403
Detection of protease and protease activity using a single nanoscrescent SERS probe
patent, January 2013
- Liu, Gang; Ellman, Jonathan A.; Lee, Luke P.
- US Patent Document 8,361,932
Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity
patent, April 2014
- Zhang, Xiang; Ellman, Jonathan A.; Chen, Fanqing Frank
- US Patent Document 8,685,743
Development and validation of a quantitative ELISA for the measurement of PSA concentration
journal, March 2002
- Acevedo, Boris; Perera, Yasser; Ruiz, Miriam
- Clinica Chimica Acta, Vol. 317, Issue 1-2, p. 55-63
Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections
journal, May 1999
- Bjartell, A.; Siivola, P.; Hulkko, S.
- Prostate Cancer and Prostatic Diseases, Vol. 2, Issue 3
Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates: Amidolytic activity of prostatic acid phosphatase
journal, January 2002
- Brillard-Bourdet, Michèle; Réhault, Sophie; Juliano, Luiz
- European Journal of Biochemistry, Vol. 269, Issue 1
Two-dimensional electrophoresis of prostate-specific antigen in sera of men with prostate cancer or benign prostate hyperplasia
journal, January 1999
- Charrier, Jean-Philippe; Tournel, Carole; Michel, Sandrine
- Electrophoresis, Vol. 20, Issue 4-5, p. 1075-1081
Epidemiology of prostate cancer
journal, December 2003
- Crawford, E. David
- Urology, Vol. 62, Issue 6, p. 3-12
A history of prostate cancer treatment
journal, May 2002
- Denmeade, Samuel R.; Isaacs, John T.
- Nature Reviews Cancer, Vol. 2, Issue 5
Prostate-Specific Antigen-Activated Thapsigargin Prodrug as Targeted Therapy for Prostate Cancer
journal, July 2003
- Denmeade, S. R.; Jakobsen, C. M.; Janssen, S.
- JNCI Journal of the National Cancer Institute, Vol. 95, Issue 13
The role of prostate-specific antigen in the clinical evaluation of prostatic disease
journal, March 2004
- Denmeade, S. R.; Isaacs, J. T.
- BJU International, Vol. 93, Issue s1
Detection of a Biomarker for Alzheimer's Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor
journal, February 2005
- Haes, Amanda J.; Chang, Lei; Klein, William L.
- Journal of the American Chemical Society, Vol. 127, Issue 7
Surface-enhanced Raman scattering on tunable plasmonic nanoparticle substrates
journal, December 2004
- Jackson, J. B.; Halas, N. J.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 52, p. 17930-17935
Magnetic Nanocrescents as Controllable Surface-Enhanced Raman Scattering Nanoprobes for Biomolecular Imaging
journal, November 2005
- Liu, G. L.; Lu, Y.; Kim, J.
- Advanced Materials, Vol. 17, Issue 22, p. 2683-2688
Nanowell surface enhanced Raman scattering arrays fabricated by soft-lithography for label-free biomolecular detections in integrated microfluidics
journal, August 2005
- Liu, Gang L.; Lee, Luke P.
- Applied Physics Letters, Vol. 87, Issue 7
Optofluidic control using photothermal nanoparticles
journal, December 2005
- Liu, Gang L.; Kim, Jaeyoun; Lu, Yu
- Nature Materials, Vol. 5, Issue 1
High-Density Silver Nanoparticle Film with Temperature-Controllable Interparticle Spacing for a Tunable Surface Enhanced Raman Scattering Substrate
journal, January 2005
- Lu, Yu; Liu, Gang L.; Lee, Luke P.
- Nano Letters, Vol. 5, Issue 1
Nanophotonic Crescent Moon Structures with Sharp Edge for Ultrasensitive Biomolecular Detection by Local Electromagnetic Field Enhancement Effect
journal, December 2004
- Lu, Yu; Liu, Gang L.; Kim, Jaeyoun
- Nano Letters, Vol. 5, Issue 1
Enzymatic action of prostate-specific antigen (PSA or hK3): Substrate specificity and regulation by Zn2+, a tight-binding inhibitor
journal, October 2000
- Malm, Johan; Hellman, Jukka; Hogg, Phil
- The Prostate, Vol. 45, Issue 2, p. 132-139
Pro PSA: a more cancer specific form of prostate specific antigen for the early detection of prostate cancer.
journal, January 2003
- Mikolajczyk, Stephen D.; Rittenhouse, Harry G.
- The Keio Journal of Medicine, Vol. 52, Issue 2
Are multiple markers the future of prostate cancer diagnostics?
journal, July 2004
- Mikolajczyk, Stephen D.; Song, Yong; Wong, James R.
- Clinical Biochemistry, Vol. 37, Issue 7, p. 519-528
Proenzyme Forms of Prostate-Specific Antigen in Serum Improve the Detection of Prostate Cancer
journal, June 2004
- Mikolajczyk, S. D.
- Clinical Chemistry, Vol. 50, Issue 6
Epidemiology of prostate cancer: molecular and environmental clues
journal, November 1996
- Pienta, Kenneth J.; Goodson, Jo Ann; Esper, Peggy S.
- Urology, Vol. 48, Issue 5, p. 676-683
A Change of Wave-length in Light Scattering
journal, April 1928
- Raman, C. V.
- Nature, Vol. 121, Issue 3051
Design of new and sensitive fluorogenic substrates for human kallikrein hK3 (prostate-specific antigen) derived from semenogelin sequences
journal, April 2002
- Réhault, Sophie; Brillard-Bourdet, Michèle; Bourgeois, Luc
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1596, Issue 1
Human Kallikrein 2 (hK2) and Prostate-Specific Antigen (PSA): Two Closely Related, but Distinct, Kallikreins in the Prostate
journal, January 1998
- Rittenhouse, Harry G.; Finlay, Judith A.; Mikolajczyk, Stephen D.
- Critical Reviews in Clinical Laboratory Sciences, Vol. 35, Issue 4
Characterization of Prostate-Specific Antigen Proteolytic Activity on its Major Physiological Substrate, the Sperm Motility Inhibitor Precursor/Semenogelin I †
journal, April 1997
- Robert, Martin; Gibbs, Bernard F.; Jacobson, Elka
- Biochemistry, Vol. 36, Issue 13
Immunopeptidometric Assay for Enzymatically Active Prostate-Specific Antigen
journal, January 2004
- Wu, P.
- Clinical Chemistry, Vol. 50, Issue 1
Separation of enzymatically active and inactive prostate-specific antigen (PSA) by peptide affinity chromatography: PSA-Specific Peptides
journal, November 2003
- Wu, Ping; Stenman, Ulf-Håkan; Pakkala, Miikka
- The Prostate, Vol. 58, Issue 4
The New Human Tissue Kallikrein Gene Family: Structure, Function,
and Association to Disease*
journal, April 2001
- Yousef, George M.; Diamandis, Eleftherios P.
- Endocrine Reviews, Vol. 22, Issue 2