Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter

Gibney, Patrick A.; Schieler, Ariel; Chen, Jonathan C.; Rabinowitz, Joshua D.; Botstein, David

doi:10.1073/pnas.1506289112

Title: Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter

Journal Article · Mon Apr 27 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1506289112· OSTI ID:1347586

Gibney, Patrick A. ^[1]; Schieler, Ariel ^[1]; Chen, Jonathan C. ^[1]; Rabinowitz, Joshua D. ^[1]; Botstein, David ^[1]

Princeton Univ., Princeton, NJ (United States)

Trehalose is a highly stable, nonreducing disaccharide of glucose. A large body of research exists implicating trehalose in a variety of cellular phenomena, notably response to stresses of various kinds. However, in very few cases has the role of trehalose been examined directly in vivo. Here, we describe the development and characterization of a system in Saccharomyces cerevisiae that allows us to manipulate intracellular trehalose concentrations independently of the biosynthetic enzymes and independently of any applied stress. We found that many physiological roles heretofore ascribed to intracellular trehalose, including heat resistance, are not due to the presence of trehalose per se. We also found that many of the metabolic and growth defects associated with mutations in the trehalose biosynthesis pathway are not abolished by providing abundant intracellular trehalose. Instead, we made the observation that intracellular accumulation of trehalose or maltose (another disaccharide of glucose) is growth-inhibitory in a carbon source-specific manner. We conclude that the physiological role of the trehalose pathway is fundamentally metabolic: i.e., more complex than simply the consequence of increased concentrations of the sugar and its attendant physical properties (with the exception of the companion paper where demonstrate a direct role for trehalose in protecting cells against desiccation).

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Research Organization:: Princeton Univ., NJ (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012461

OSTI ID:: 1347586

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 19; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 58 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
yeast
trehalose
AGT1
heat shock
carbon source

Title: Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter

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