Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis

Jin, Qiaoling; Vogt, Stefan; Lai, Barry; Chen, Si; Finney, Lydia; Gleber, Sophie -Charlotte; Ward, Jesse; Deng, Junjing; Mak, Rachel; Moonier, Nena; Jacobsen, Chris; Brody, James P.

doi:10.1371/journal.pone.0117437

Title: Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis

Journal Article · Mon Feb 23 00:00:00 EST 2015 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0117437· OSTI ID:1344551

Jin, Qiaoling ^[1]; Vogt, Stefan ^[2]; Lai, Barry ^[2]; Chen, Si ^[2]; Finney, Lydia ^[2]; Gleber, Sophie -Charlotte ^[2]; Ward, Jesse ^[2]; Deng, Junjing ^[1]; Mak, Rachel ^[1]; Moonier, Nena ^[2]; Jacobsen, Chris ^[3]; Brody, James P. ^[4]

Northwestern Univ., Evanston, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)
Northwestern Univ., Evanston, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Univ. of California, Irvine, CA (United States)

Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH) - National Institute of General Medical Sciences; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1344551

Alternate ID(s):: OSTI ID: 1194125; OSTI ID: 1396030

Journal Information:: PLoS ONE, Vol. 10, Issue 2; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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Visualizing Metal Content and Intracellular Distribution in Primary Hippocampal Neurons with Synchrotron X-Ray Fluorescence Colvin, Robert A.; Jin, Qiaoling; Lai, Barry PLOS ONE, Vol. 11, Issue 7 https://doi.org/10.1371/journal.pone.0159582	journal	July 2016

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