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Title: Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression

Journal Article · · Plant Biotechnology Journal
DOI:https://doi.org/10.1111/pbi.12666· OSTI ID:1338528
 [1];  [1];  [1];  [1];  [1];  [2];  [3];  [2];  [2];  [4];  [4];  [5];  [4];  [4];  [2];  [3];  [4];  [5];  [1];  [1] more »;  [1] « less
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of North Texas, Denton, TX (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); The Samuel Roberts Noble Foundation, Ardmore, OK (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)
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Five different types of transgenic (GAUT4, miRNA, MYB4, COMT and FPGS) Panicum virgatum L. (switchgrass) were grown in a field in Knoxville, Tenn., USA over two consecutive years between 2011 and 2015 in separate experiments. Clonal replicates were established (year-one) and produced much greater biomass during the second year. After each growing season the above ground biomass was analyzed for cell wall sugars and for recalcitrance to enzymatic digestibility, and biofuel using a separate hydrolysis and fermentation (SHF) screen. Here, each transgenic event and control had more glucan, xylan and less ethanol (g/g basis) from the second year of growth relative to the first year plants. There was no correlation between plant carbohydrate content and biofuel production. In each of cell wall-targeted transgenics, GAUT4, MYB4, COMT and FPGS, the second year of growth resulted in increased carbohydrate abundance (up to 12%) and reduced recalcitrance through higher ethanol yields (up to 21%) over the non-transgenic control plants.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1338528
Journal Information:
Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal; ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied BiologyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 23 works
Citation information provided by
Web of Science

References (27)

Transgenic switchgrass ( Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: a 2-year comparative analysis of field-grown lines modified for target gene or genetic element expression journal February 2017
Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review journal January 2011
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Cited By (11)

Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis journal February 2018
Gene regulatory networks for lignin biosynthesis in switchgrass (Panicum virgatum ) journal September 2018
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii journal June 2017
Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells journal November 2017
Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release journal November 2017
Development and use of a switchgrass (Panicum virgatum L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance journal December 2017
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass journal April 2018
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass journal January 2019
Progress in understanding and overcoming biomass recalcitrance: a BioEnergy Science Center (BESC) perspective journal November 2017
Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death journal June 2017
Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food? journal December 2019

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