Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations

He, Chiyang; Zhu, Zaifang; Gu, Congying; Lu, Joann; Liu, Shaorong

doi:10.1016/j.chroma.2011.12.105

Title: Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations

Journal Article · Mon Jan 09 00:00:00 EST 2012 · Journal of Chromatography

DOI:https://doi.org/10.1016/j.chroma.2011.12.105· OSTI ID:1337174

He, Chiyang ^[1]; Zhu, Zaifang ^[2]; Gu, Congying ^[2]; Lu, Joann ^[2]; Liu, Shaorong ^[2]

Wuhan Textile Univ., Wuhan (China)
Univ. of Oklahoma, Norman, OK (United States)

Numerous micropumps have been developed, but few of them can produce adequate flow rate and pressure for high-performance liquid chromatography (HPLC) applications. We have recently developed an innovative hybrid electroosmotic pump (EOP) to solve this problem. The basic unit of a hybrid pump consists of a +EOP (the pumping element is positively charged) and a -EOP (the pumping element is negatively charged). The outlet of the +EOP is then joined with the inlet of the -EOP, forming a basic pump unit, while the anode of a positive high voltage (HV) power supply is placed at the joint. The inlet and outlet of this pump unit are electrically grounded. With this configuration, we can stack many of such pump units in series to boost the pumping power. In this work, we describe in details how an open-capillary hybrid EOP is constructed and characterize this pump systematically. We also show that a hybrid EOP with ten serially stacked pump units can deliver a maximum pressure of 21.5 MPa (~3100 psi). Here, we further demonstrate the feasibility of using this hybrid EOP to drive eluents for HPLC separations of proteins and peptides.

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Research Organization:: MicroChem Solutions, Norman, OK (United States)

Sponsoring Organization:: USDOE

Contributing Organization:: University of Oklahoma

Grant/Contract Number:: SC0006351

OSTI ID:: 1337174

Journal Information:: Journal of Chromatography, Vol. 1227, Issue C; ISSN 0021-9673

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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Title: Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations

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