IC Post-Doctoral Fellowship Abstract and Bio.

Lacey, Randy

doi:10.2172/1333518

Title: IC Post-Doctoral Fellowship Abstract and Bio.

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Abstract

Whole cell biosensors (WCBs) utilize an organism’s natural ability to sense and respond to the environment. Through implementation of two different protein engineering methods, I seek to develop a WCB for the detection of important chemical signatures in the environment. I will reengineer the ligand binding profile of proteins known to alter transcription of genes, and I will engineer signal transduction in proteins already known to bind relevant compounds. In both cases, detection of compounds of interest will lead to the production of a measurable fluorescent signal within the organism. These approaches will provide the groundwork for the development of novel chemical sensing technologies that provide a cheap and efficient alternative to traditional methods for detection of compounds.

Authors:

Lacey, Randy ^[1]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Publication Date:: Tue Nov 01 00:00:00 EDT 2016

Research Org.:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1333518

Report Number(s):: SAND-2016-11823R
649373

DOE Contract Number:: AC04-94AL85000

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

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                    Lacey, Randy. IC Post-Doctoral Fellowship Abstract and Bio..  United States: N. p., 2016. 
        Web.  doi:10.2172/1333518.

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                    Lacey, Randy. IC Post-Doctoral Fellowship Abstract and Bio..  United States.  https://doi.org/10.2172/1333518

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                    Lacey, Randy. 2016.  
        "IC Post-Doctoral Fellowship Abstract and Bio.".  United States.  https://doi.org/10.2172/1333518.  https://www.osti.gov/servlets/purl/1333518.

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@article{osti_1333518,

  title        = {IC Post-Doctoral Fellowship Abstract and Bio.},

  author       = {Lacey, Randy},

  abstractNote = {Whole cell biosensors (WCBs) utilize an organism’s natural ability to sense and respond to the environment. Through implementation of two different protein engineering methods, I seek to develop a WCB for the detection of important chemical signatures in the environment. I will reengineer the ligand binding profile of proteins known to alter transcription of genes, and I will engineer signal transduction in proteins already known to bind relevant compounds. In both cases, detection of compounds of interest will lead to the production of a measurable fluorescent signal within the organism. These approaches will provide the groundwork for the development of novel chemical sensing technologies that provide a cheap and efficient alternative to traditional methods for detection of compounds.},

  doi          = {10.2172/1333518},

  url          = {https://www.osti.gov/biblio/1333518},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Nov 01 00:00:00 EDT 2016},

  month        = {Tue Nov 01 00:00:00 EDT 2016}

}

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https://doi.org/10.2172/1333518

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