Epigenetic Regulation of Hormone-dependent Plant Growth Processes
- The Salk Inst. for Biological Studies, La Jolla, CA (United States)
Impact of EIN6, EEN and ethylene on the H3K27me3 dynamics in Arabidopsis: To assess the dynamic responsiveness of H3K27me3 levels to ethylene and how this might affect ethylene-induced gene expression, we plan to perform H3K27me3 ChIP-seq and RNA- seq experiments in parallel with etiolated seedlings in the absence and presence of ethylene. Further implementation of ein6, een and ein6een mutants will visualize how the H3K27me3 landscape (-/+ET) is altered when H3K27me3 demethylation and/or INO80-mediated chromatin remodeling is compromised. Additional ChIP-seq analyses with EIN6 will show if ethylene- induced H3K27me3 removal at certain genes is always accompanied by the presence of EIN6.
- Research Organization:
- The Salk Inst. for Biological Studies, La Jolla, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- DOE Contract Number:
- FG02-04ER15517
- OSTI ID:
- 1332760
- Report Number(s):
- DOE-SALK-15517
- Country of Publication:
- United States
- Language:
- English
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