Directed plant cell-wall accumulation of iron: Embedding co-catalyst for efficient biomass conversion

Lin, Chien -Yuan; Jakes, Joseph E.; Donohoe, Bryon S.; Ciesielski, Peter N.; Yang, Haibing; Gleber, Sophie -Charlotte; Vogt, Stefan; Ding, Shi -You; Peer, Wendy A.; Murphy, Angus S.; McCann, Maureen C.; Himmel, Michael E.; Tucker, Melvin P.; Wei, Hui

doi:10.1186/s13068-016-0639-2

Title: Directed plant cell-wall accumulation of iron: Embedding co-catalyst for efficient biomass conversion

Journal Article · Fri Oct 21 00:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0639-2· OSTI ID:1331239

Lin, Chien -Yuan ^[1]; Jakes, Joseph E. ^[2]; Donohoe, Bryon S. ^[1]; Ciesielski, Peter N. ^[1]; Yang, Haibing ^[3]; Gleber, Sophie -Charlotte ^[4]; Vogt, Stefan ^[4]; Ding, Shi -You ^[5]; Peer, Wendy A. ^[6]; Murphy, Angus S. ^[6]; McCann, Maureen C. ^[3]; Himmel, Michael E. ^[1]; Tucker, Melvin P. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
USDA Forest Service, Madison, WI (United States)
Purdue Univ., West Lafayette, IN (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Michigan State Univ., East Lansing, MI (United States)
Univ. of Maryland, College Park, MD (United States)

Plant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However, directly adding iron catalysts into biomass prior to pretreatment is diffusion limited, and increases the cost of biorefinery operations. Recently, we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this study, we extend this approach by fusing the heterologous ferritin gene with a signal peptide for secretion into Arabidopsis cell walls (referred to here as FerEX).

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); US Department of Agriculture (USDA)

Grant/Contract Number:: AC36-08GO28308; SC000997; AC02-06CH11357

OSTI ID:: 1331239

Alternate ID(s):: OSTI ID: 1377621

Report Number(s):: NREL/JA-2700-67070

Journal Information:: Biotechnology for Biofuels, Vol. 9, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion Lin, Chien-Yuan; Jakes, Joseph; Donohoe, Bryon https://doi.org/10.6084/m9.figshare.c.3623552 The current record is supplemented by this dataset	dataset	October 2016
MOESM1 of Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion Jakes, Joseph; Donohoe, Bryon; Ciesielski, Peter https://doi.org/10.6084/m9.figshare.c.3623552_d1.v1 The current record is supplemented by this figure	figure	October 2016
Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion [Supplemental Data] Lin, Chien-Yuan; Jakes, Joseph; Donohoe, Bryon https://doi.org/10.6084/m9.figshare.c.3623552.v1 The current record is supplemented by this dataset	dataset	October 2016
MOESM1 of Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion Lin, Chien-Yuan; Jakes, Joseph; Donohoe, Bryon https://doi.org/10.6084/m9.figshare.c.3623552_d1 The current record is supplemented by this text	text	January 2016