Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines

Lin, Jingyu; Mazarei, Mitra; Zhao, Nan; Hatcher, Catherine N.; Wuddineh, Wegi A.; Rudis, Mary; Tschaplinski, Timothy J.; Pantalone, Vincent R.; Arelli, Prakash R.; Hewezi, Tarek; Chen, Feng; Stewart, Jr., Charles Neal

doi:10.1111/pbi.12566

Title: Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines

Journal Article · Mon May 23 00:00:00 EDT 2016 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12566· OSTI ID:1329152

Lin, Jingyu ^[1]; Mazarei, Mitra ^[1]; Zhao, Nan ^[2]; Hatcher, Catherine N. ^[1]; Wuddineh, Wegi A. ^[1]; Rudis, Mary ^[1]; Tschaplinski, Timothy J. ^[2]; Pantalone, Vincent R. ^[1]; Arelli, Prakash R. ^[3]; Hewezi, Tarek ^[1]; Chen, Feng ^[1]; Stewart, Jr., Charles Neal ^[1]

Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
USDA-ARS, Jackson, TN (United States)

Soybean (Glycine max (L.) Merr.) salicylic acid methyl transferase (GmSAMT1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when GmSAMT1 was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (SCN), Heterodera glycines Ichinohe. In this study, we produced transgenic soybean overexpressing GmSAMT1 and characterized their response to various SCN races. Transgenic plants conferred a significant reduction in the development of SCN HG type 1.2.5.7 (race 2), HG type 0 (race 3) and HG type 2.5.7 (race 5). Among transgenic lines, GmSAMT1 expression in roots was positively associated with SCN resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22 °C day/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27 °C day/23 °C night) temperature. In a 1-year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible SCN infection. We hypothesize that GmSAMT1 expression affects salicylic acid biosynthesis, which, in turn, attenuates SCN development, without negative consequences to soybean yield or other morphological traits. Furthermore, we conclude that GmSAMT1 overexpression confers broad resistance to multiple SCN races, which would be potentially applicable to commercial production.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1329152

Journal Information:: Plant Biotechnology Journal, Vol. 14, Issue 11; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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Title: Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines

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