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Title: Isolating a functionally relevant guild of fungi from the root microbiome of Populus

Journal Article · · Fungal Ecology
 [1];  [2];  [2];  [2];  [3];  [2]
  1. Michigan State Univ., East Lansing, MI (United States)
  2. Duke Univ., Durham, NC (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
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Plant roots interact with a bewilderingly complex community of microbes, including root-associated fungi that are essential for maintaining plant health. To improve understanding of the diversity of fungi in the rhizobiome of Populus deltoides, Populus trichocarpa and co-occurring plant hosts Quercus alba and Pinus taeda, we conducted field and greenhouse studies and sampled, isolated, and characterized the diversity of culturable root-associated fungi on these hosts. Using both general and selective isolation media we obtained more than 1800 fungal isolates from individual surface sterilized root tips. Sequences from the ITS and/or D1– D2 regions of the LSU rDNA were obtained from 1042 of the >1800 pure culture isolates and were compared to accessions in the NCBI nucleotide database and analyzed through phylogenetics for preliminary taxonomic identification. Sequences from these isolates were also compared to 454 sequence datasets obtained directly from the Populus rhizosphere and endosphere. Although most of the ectomycorrhizal taxa known to associate with Populus evaded isolation, many of the abundant sequence types from rhizosphere and endosphere 454 datasets were isolated, including novel species belonging to the Atractiellales. Isolation and identification of key endorrhizal fungi will enable more targeted study of plant-fungal interactions. Genome sequencing is currently underway for a subset of our culture library with the aim of understanding the mechanisms involved in host-endophyte establishment and function. As a result, this diverse culture library of fungal root associates will be a valuable resource for metagenomic research, experimentation and further studies on plant-fungal interactions.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1327688
Alternate ID(s):
OSTI ID: 1328482
Journal Information:
Fungal Ecology, Vol. 22, Issue C; ISSN 1754-5048
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 54 works
Citation information provided by
Web of Science

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Mycoplasma-related endobacteria within Mortierellomycotina fungi: diversity, distribution and functional insights into their lifestyle journal February 2018
Time to re-think fungal ecology? Fungal ecological niches are often prejudged journal January 2018
Distinct Communities of Poplar Endophytes on an Unpolluted and a Risk Element-Polluted Site and Their Plant Growth-Promoting Potential In Vitro journal November 2017
Impact of soil pedogenesis on the diversity and composition of fungal communities across the California soil chronosequence of Mendocino journal March 2018
Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata journal June 2018
Comparative genomics of Mortierella elongata and its bacterial endosymbiont Mycoavidus cysteinexigens journal February 2017
A plant growth‐promoting bacterium alters the microbial community of continuous cropping poplar trees’ rhizosphere journal January 2019
Host species identity in annual Brassicaceae has a limited effect on the assembly of root-endophytic fungal communities journal September 2018
Plant growth promotion, metabolite production and metal tolerance of dark septate endophytes isolated from metal-polluted poplar phytomanagement sites journal June 2016
Differential growth promotion of poplar and birch inoculated with three dark septate endophytes in two trace element-contaminated soils journal May 2017
Potential of a halophyte-associated endophytic fungus for sustaining Chinese white poplar growth under salinity journal February 2018
Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype journal March 2019
Metabolomics-based chemotaxonomy of root endophytic fungi for natural products discovery: Chemotaxonomy of root endophytic fungi journal March 2018
Phylogenetic and morphological analyses of the mycoparasitic genus  Piptocephalis journal January 2019
Structural plasticity in root-fungal symbioses: diverse interactions lead to improved plant fitness journal December 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Populus deltoides
Populus trichocarpa
endophytes
ectomycorrhizal fungi
ecological interactions
rhizobiome