Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol

Frisz, Jessica F.; Klitzing, Haley A.; Lou, Kaiyan; Hutcheon, Ian D.; Weber, Peter K.; Zimmerberg, Joshua; Kraft, Mary L.

doi:10.1074/jbc.M113.473207

Title: Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol

Journal Article · Mon Apr 22 00:00:00 EDT 2013 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.M113.473207· OSTI ID:1313553

Frisz, Jessica F. ^[1]; Klitzing, Haley A. ^[1]; Lou, Kaiyan ^[1]; Hutcheon, Ian D. ^[2]; Weber, Peter K. ^[2]; Zimmerberg, Joshua ^[3]; Kraft, Mary L. ^[4]

Univ. of Illinois, Urbana, IL (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
National Institutes of Health, Bethesda, MD (United States)
Univ. of Illinois, Urbana, IL (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the plasma membranes of intact fibroblast cells. Although acute cholesterol depletion reduced sphingolipid domain abundance, cholesterol was evenly distributed throughout the plasma membrane and was not enriched within the sphingolipid domains. As a result, we rule out favorable cholesterol-sphingolipid interactions as dictating plasma membrane organization in fibroblast cells. Because the sphingolipid domains are disrupted by drugs that depolymerize the cells actin cytoskeleton, cholesterol must instead affect the sphingolipid organization via an indirect mechanism that involves the cytoskeleton.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1313553

Report Number(s):: LLNL-JRNL-638518

Journal Information:: Journal of Biological Chemistry, Vol. 288, Issue 23; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 110 works

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Title: Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol

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