Bioaccumulation of Multiwall Carbon Nanotubes in Tetrahymena thermophila by Direct Feeding or Trophic Transfer

Mortimer, Monika; Petersen, Elijah J.; Buchholz, Bruce A.; Orias, Eduardo; Holden, Patricia A.

doi:10.1021/acs.est.6b01916

Title: Bioaccumulation of Multiwall Carbon Nanotubes in Tetrahymena thermophila by Direct Feeding or Trophic Transfer

Journal Article · Mon Jul 11 00:00:00 EDT 2016 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.6b01916· OSTI ID:1305849

Mortimer, Monika ^[1]; Petersen, Elijah J. ^[2]; Buchholz, Bruce A. ^[3]; Orias, Eduardo ^[4]; Holden, Patricia A. ^[5]

Univ. of California, Santa Barbara, CA (United States). Center for the Environmental Implications of Nanotechnology (UC CEIN), Earth Research Inst., Bren School of Environmental Science and Management; National Inst. of Chemical Physics and Biophysics, Tallinn (Estonia). Lab. of Environmental Toxicology
National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Biosystems and Biomaterials Division
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Center for Accelerator Mass Spectrometry
Univ. of California, Santa Barbara, CA (United States). Dept. of Molecular, Cellular, and Developmental Biology
Univ. of California, Santa Barbara, CA (United States). Center for the Environmental Implications of Nanotechnology (UC CEIN), Earth Research Inst., Bren School of Environmental Science and Managemen

We report that consumer goods contain multiwall carbon nanotubes (MWCNTs) that could be released during product life cycles into the environment, where their effects are uncertain. Here, we assessed MWCNT bioaccumulation in the protozoan Tetrahymena thermophila via trophic transfer from bacterial prey (Pseudomonas aeruginosa) versus direct uptake from growth media. The experiments were conducted using ¹⁴C-labeled MWCNT (¹⁴C-MWCNT) doses at or below 1 mg/L, which proved subtoxic since there were no adverse effects on the growth of the test organisms. A novel contribution of this study was the demonstration of the ability to quantify MWCNT bioaccumulation at low (sub μg/kg) concentrations accomplished by employing accelerator mass spectrometry (AMS). After the treatments with MWCNTs at nominal concentrations of 0.01 mg/L and 1 mg/L, P. aeruginosa adsorbed considerable amounts of MWCNTs: (0.18 ± 0.04) μg/mg and (21.9 ± 4.2) μg/mg bacterial dry mass, respectively. At the administered MWCNT dose of 0.3 mg/L, T. thermophila accumulated up to (0.86 ± 0.3) μg/mg and (3.4 ± 1.1) μg/mg dry mass by trophic transfer and direct uptake, respectively.Finally, aAlthough MWCNTs did not biomagnify in the microbial food chain, MWCNTs bioaccumulated in the protozoan populations regardless of the feeding regime, which could make MWCNTs bioavailable for organisms at higher trophic levels.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344; PUTJD16

OSTI ID:: 1305849

Report Number(s):: LLNL-JRNL-681494

Journal Information:: Environmental Science and Technology, Vol. 50, Issue 16; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 50 works

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Title: Bioaccumulation of Multiwall Carbon Nanotubes in Tetrahymena thermophila by Direct Feeding or Trophic Transfer

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