Method for genetic identification of unknown organisms

Colston, Jr., Billy W.; Fitch, Joseph P.; Hindson, Benjamin J.; Carter, Chance J.; Beer, Neil Reginald

Title: Method for genetic identification of unknown organisms

Patent · Tue Aug 23 00:00:00 EDT 2016

OSTI ID:1303664

Colston, Jr., Billy W.; Fitch, Joseph P.; Hindson, Benjamin J.; Carter, Chance J.; Beer, Neil Reginald

A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: 9,422,586

Application Number:: 11/818,623

OSTI ID:: 1303664

Resource Relation:: Patent File Date: 2007 Jun 15

Country of Publication:: United States

Language:: English

References (10)

Chip-based species identification and phenotypic characterization of microorganisms Gingeras, Thomas; Mack, David; Chee, Mark S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6924094 US Patent Document 6,924,094	patent	August 2005
Methods and systems for control of microfluidic devices Handique, Kalyan; Ganesan, Karthik; Brahmasandra, Sundaresh N. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7010391 US Patent Document 7,010,391	patent	March 2006
Chemical amplification based on fluid partitioning Anderson, Brian L.; Colston, JR., Billy W.; Elkin, Chris https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7041481 US Patent Document 7,041,481	patent	May 2006
Integrated system for diversity generation and screening Bass, Steven H.; Davis, S. Christopher; Patten, Phillip A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7462469 US Patent Document 7,462,469	patent	December 2008
Amplification of complex gene libraries by emulsion PCR Williams, Richard; Peisajovich, Sergio G.; Miller, Oliver J. Nature Methods, Vol. 3, Issue 7, p. 545-550 https://doi.org/10.1038/nmeth896	journal	July 2006
BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions Diehl, Frank; Li, Meng; He, Yiping Nature Methods, Vol. 3, Issue 7 https://doi.org/10.1038/nmeth898	journal	July 2006
APPLIED PHYSICS: Droplet Control for Microfluidics Joanicot, M. Science, Vol. 309, Issue 5736 https://doi.org/10.1126/science.1112615	journal	August 2005
Miniemulsion Droplets as Single Molecule Nanoreactors for Polymerase Chain Reaction Musyanovych, Anna; Mailänder, Volker; Landfester, Katharina Biomacromolecules, Vol. 6, Issue 4 https://doi.org/10.1021/bm050084+	journal	June 2005
Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets He, Mingyan; Edgar, J. Scott; Jeffries, Gavin D. M. Analytical Chemistry, Vol. 77, Issue 6 https://doi.org/10.1021/ac0480850	journal	March 2005
Single-molecule PCR using water-in-oil emulsion Nakano, Michihiko; Komatsu, Jun; Matsuura, Shun-ichi Journal of Biotechnology, Vol. 102, Issue 2, p. 117-124 https://doi.org/10.1016/S0168-1656(03)00023-3	journal	April 2003

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