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Title: Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana

Journal Article · · Algal Research
 [1];  [1];  [1];  [2];  [1];  [3];  [1];  [1]
  1. Washington State Univ., Pullman, WA (United States)
  2. Johns Hopkins Univ., Baltimore, MD (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
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Mixotrophic growth of microalgae offers great potential as an efficient strategy for biofuel production. In this study, photosynthetic regulation of mixotrophically cultured Chlorella sorokiniana cells was systematically evaluated. Mixotrophic cells in the exponential growth phase showed the highest photosynthetic activity, where maximum photosynthetic O2 evolution was approximately 3- and 4-fold higher than cells in the same phase grown photoautotrophically in 1% CO2 (in air) and air, respectively. Additionally, characteristic chlorophyll fluorescence parameters demonstrated that no limitation in electron transport downstream of PSII was detected in mixotrophic cells. Up-regulation of photosynthetic activity was associated with high total ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylase activity and expression level of phosphoribulokinase (PRK). After 3 days, photosynthetic O2 evolution of mixotrophic cells that went to the stationary phase, was strongly reduced, with reduced photochemical efficiency and reorganization of the PSII complex. Simultaneously, enzymatic activity for Rubisco carboxylase and mRNA levels of Rubisco and PRK diminished. Importantly, there was almost no non-photochemical quenching for mixotrophic cells, whether grown in log or stationary phase. A decline in the quantum efficiency of PSII and an oxidized plastoquinone pool (PQ pool) was observed under N-depleted conditions during mixotrophic growth. Finally, these results demonstrate that photosynthesis is regulated differently in mixotrophically cultured C. sorokiniana cells than in cells grown under photoautotrophic conditions, with a particularly strong impact by nitrogen levels in the cells.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AC36-08GO28308; EE0003112
OSTI ID:
1290785
Alternate ID(s):
OSTI ID: 1358745
Report Number(s):
NREL/JA-5100-66919
Journal Information:
Algal Research, Vol. 19; ISSN 2211-9264
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 38 works
Citation information provided by
Web of Science

References (40)

Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production journal July 2014
Mixotrophic growth ofChlorella sorokiniana in outdoor enclosed photobioreactor journal March 1996
Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation journal April 2012
System Response of Metabolic Networks in Chlamydomonas reinhardtii to Total Available Ammonium journal July 2012
Dynamic flexibility in the light reactions of photosynthesis governed by both electron and proton transfer reactions journal July 2004
Effects of Acetate on Facultative Autotrophy in Chlamydomonas reinhardtii Assessed by Photosynthetic Measurements and Stable Isotope Analyses journal April 2000
Dark Induction of the Non-Photochemical Quenching of Chlorophyll Fluorescence by Acetate in Chlamydomonas reinhardtii journal June 1996
Effects of Light and Glycerol on the Organization of the Photosynthetic Apparatus in the Facultative Heterotroph Pyrenomonas Salina (Cryptophyceae)1 journal October 1991
Effects of organic carbon sources on growth, photosynthesis, and respiration of Phaeodactylum tricornutum journal September 2008
Interactions between organic and inorganic carbon sources during mixotrophic cultivation of Synechococcus sp. journal September 2004
The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii journal January 1986
Expression of a gene for a light-harvesting chlorophyll a/b-binding protein in Chlamydomonas reinhardtii: effect of light and acetate journal January 1987
Photo- and Metabolite Regulation of the Synthesis of Ribulose Bisphosphate Carboxylase/Oxygenase and the Phycobiliproteins in the Alga Cyanidium caldarium journal December 1984
Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria†: Regulation of photosynthesis in Galdieria journal June 2007
Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid journal January 1975
Measurement and Interpretation of Photosynthetic Light-Response Curves in Algae in the Context of Photoinhibition and diel Changes journal December 1993
Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo journal June 2008
Structural and functional self-organization of Photosystem II in grana thylakoids journal September 2007
Spatial variation in photosynthetic CO2 carbon and oxygen isotope discrimination along leaves of the monocot triticale (Triticum × Secale) relates to mesophyll conductance and the Péclet effect: Spatial variation in photosynthetic discrimination journal June 2011
Temperature response of in vivo  Rubisco kinetics and mesophyll conductance in Arabidopsis thaliana : comparisons to Nicotiana tabacum journal August 2013
Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana journal March 2015
Photosystem-II repair and chloroplast recovery from irradiance stress: relationship between chronic photoinhibition, light-harvesting chlorophyll antenna size and photosynthetic productivity in Dunaliella salina (green algae) journal May 1998
Non-Photochemical Quenching. A Response to Excess Light Energy journal April 2001
An ancient light-harvesting protein is critical for the regulation of algal photosynthesis journal November 2009
Differential thermal effects on the energy distribution between photosystem II and photosystem I in thylakoid membranes of a psychrophilic and a mesophilic alga journal April 2002
Efficient Light Harvesting by Photosystem II Requires an Optimized Protein Packing Density in Grana Thylakoids journal April 2010
Coordination of photosynthetic and respiratory metabolism in Chlorella vulgaris UAM 101 in the light journal August 1995
Uptake and Utilization of Fructose by Anabaena variabilis ATCC 29413. Effect on Respiration and Photosynthesis journal April 1992
Regulation of Periplasmic Carbonic Anhydrase Expression in Chlamydomonas reinhardtii by Acetate and pH journal September 1994
Thylakoid Membrane Reduction Affects the Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp. PCC 6803 journal December 2008
ATP control on state transitions in vivo in Chlamydomonas reinhardtii journal October 1990
Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials journal May 2011
Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions journal March 2009
Effects of Inorganic n Availability on Algal Photosynthesis and Carbon Metabolism journal February 1991
Interactions Among Irradiance, Oxygen Evolution and Nitrite Uptake by Chlamydomonas (Chlorophyceae)1,2 journal December 1987
Carbon metabolite feedback regulation of leaf photosynthesis and development journal January 2003
The nitrate and ammonium nitrate supply have a major influence on the response of photosynthesis, carbon metabolism, nitrogen metabolism and growth to elevated carbon dioxide in tobacco journal October 1999
Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability journal February 2002
Acclimation of photosynthesis to elevated CO2 through feedback regulation of gene expression: Climate of opinion journal June 1996
Increased capacity for photosynthesis in wheat grown at elevated CO2: the relationship between electron transport and carbon metabolism journal October 1995

Cited By (5)

Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana journal April 2018
Cultivation of Chlorella vulgaris in sludge extract from resorcinol-rich wastewater: the removal and inhibitory effect of sludge toxicity: Cultivation of Chlorella vulgaris in sludge extract from resorcinol-rich wastewater journal January 2019
Cell growth and lipid accumulation of a microalgal mutant Scenedesmus sp. Z-4 by combining light/dark cycle with temperature variation journal November 2017
Glucose addition-induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri: Impacts of glucose addition in Chlorella kessleri journal December 2018
Respirometry as a tool to quantify kinetic parameters of microalgal mixotrophic growth journal February 2019

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
microalgae
Chlorella sorokiniana
mixotrophic
photosynthesis