skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting

Journal Article · · Lab on a chip (Print)
DOI:https://doi.org/10.1039/c4lc01342e· OSTI ID:1282170
 [1];  [2];  [3];  [4];  [5]
  1. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States)
  2. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boston Univ., Boston, MA (United States). Dept. of Biomedical Engineering
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  5. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States); Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering; Univ. of California, San Francisco, CA (United States). California Inst. for Quantitative Biosciences
+ Show Author Affiliations

Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min-1) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. Finally, the approach is generalizable to other microfluidic platforms for rapid, high-throughput analysis.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE; National Institutes of Health (NIH)
Grant/Contract Number:
AC52-07NA27344; 14-LW-077; 1DP1DE024408-01; 1R21A1109611-01A1
OSTI ID:
1282170
Journal Information:
Lab on a chip (Print), Vol. 15, Issue 4; ISSN 1473-0197
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 5 works
Citation information provided by
Web of Science

References (12)

Ultrasonic Separation of Particulate Fluids in Small and Large Scale Systems: A Review journal November 2013
Acoustophoretic Sorting of Viable Mammalian Cells in a Microfluidic Device journal November 2012
Transportation of single cell and microbubbles by phase-shift introduced to standing leaky surface acoustic waves journal December 2011
An On-Chip, Multichannel Droplet Sorter Using Standing Surface Acoustic Waves journal May 2013
Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis journal August 2012
Mode-switching: A new technique for electronically varying the agglomeration position in an acoustic particle manipulator journal January 2010
A Study of Ultrasonic Velocity and Absorption in Liquid Mixtures journal March 1948
On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves journal June 2012
Velocity of Sound in Glycerol journal January 1954
Acoustofluidics 8: Applications of acoustophoresis in continuous flow microsystems journal January 2012
Acoustofluidics 7: The acoustic radiation force on small particles journal January 2012
Manipulating particle trajectories with phase-control in surface acoustic wave microfluidics journal December 2011

Cited By (2)

Tunnel Dielectrophoresis for Tunable, Single-Stream Cell Focusing in Physiological Buffers in High-Speed Microfluidic Flows journal June 2016
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice journal November 2015

Similar Records

Related Subjects

59 BASIC BIOLOGICAL SCIENCES