Macronuclear Cytology of Synchronized Tetrahymena pyriformis
Elliott, Kennedy and Bak ('62) and Elliott ('63) followed fine structural changes in macronuclei of Tetrahymena pyriformis which were synchronized by the heat shock method of Scherbaum and Zeuthen ('54). Using Elliott's morphological descriptions as a basis, we designed our investigations with two main objectives: First, to again study the. morphological changes which occur in the macronucleus of Tetrahymena synchronized by the heat shock method. The second objective was to compare these observations with Tetrahymena synchronized by an alternate method recently reported by Padilla and Cameron ('64). Therefore, we were able to compare the results from two different synchronization methods and to contrast these findings with the macronuclear cytology of Tetrahymena taken from a logarithmically growing culture. Comparison of cells treated in these three different ways enables us to evaluate the two different synchronization methods and to gain more information on the structural changes taking place in the macronucleus of Tetrahymena as a function of the cell cycle. Our observations were confined primarily to nucleolar morphology. The results indicate that cells synchronized by the Padilla and Cameron method more closely resemble logarithmically growing Tetrahymena in the macronuclear structure than do cells obtained by the Scherbaum and·Zeuthen synchronization method. .
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- US Atomic Energy Commission (AEC)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 12777329
- Report Number(s):
- ORNL-P-1172; WorldCat 5155633525
- Journal Information:
- The Journal of Protozoology, May 1966, Vol. 13, Issue 2
- Country of Publication:
- United States
- Language:
- English
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