Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

Harris, Dustin; Ummadi, Jyothir Ganesh; Thurber, Andrew R.; Allau, Yvan; Verba, Circe; Colwell, Frederick; Torres, Marta E.; Koley, Dipankar

doi:10.1039/C6AN00007J

Title: Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

Journal Article · Fri Jan 01 00:00:00 EST 2016 · Analyst

DOI:https://doi.org/10.1039/C6AN00007J· OSTI ID:1263586

Harris, Dustin; Ummadi, Jyothir Ganesh; Thurber, Andrew R.; Allau, Yvan; Verba, Circe; Colwell, Frederick; Torres, Marta E.; Koley, Dipankar

Sporosarcina pasteurii is known to produce calcite or biocement in the presence of urea and Ca²⁺. Herein, we report the use of novel ultramicrosensors such as pH, Ca²⁺, and redox sensors, along with a scanning electrochemical microscope (SECM), to monitor a real-time, bacteria-mediated urea hydrolysis process and subsequent changes in morphology due to CaCO₃ precipitation. We report that the surface pH of a live biofilm changed rapidly from 7.4 to 9.2 within 2 min, whereas similar fast depletion (10 min) of Ca²⁺ was observed from 85 mM to 10 mM in the presence of a high urea (10 g L^-1) brine solution at 23 °C. Both the pH and the Ca²⁺ concentration profiles were extended up to 600 μm from the biofilm surface, whereas the bulk chemical composition of the brine solution remained constant over the entire 4 h of SECM experiments. In addition, we observed a change in biofilm surface morphology and an increase in overall biofilm height of 50 μm after 4 h of precipitation. Electron microscopy confirmed the changes in surface morphology and formation of CaCO₃ crystals. Development of the Ca²⁺ profile took 10 min, whereas that of the pH profile took 2 min. This finding indicates that the initial urea hydrolysis process is fast and limited by urease or number of bacteria, whereas later CaCO₃ formation and growth of crystals is a slow chemical process. The ultramicrosensors and approaches employed here are capable of accurately characterizing bioremediation on temporal and spatial scales pertinent to the microbial communities and the processes they mediate.

View Journal Article

Cite

Export

Save

Research Organization:: National Energy Technology Lab. (NETL), Albany, OR (United States)

Sponsoring Organization:: USDOE Office of Fossil Energy (FE)

OSTI ID:: 1263586

Report Number(s):: 20163; ANALAO

Journal Information:: Analyst, Vol. 141, Issue 10; ISSN 0003-2654

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

References (40)

Scanning electrochemical microscopy. 19. Ion-selective potentiometric microscopy Horrocks, Benjamin R.; Mirkin, Michael V.; Pierce, David T. Analytical Chemistry, Vol. 65, Issue 9 https://doi.org/10.1021/ac00057a019	journal	May 1993
Biofilm enhanced geologic sequestration of supercritical CO₂ Mitchell, Andrew C.; Phillips, Adrienne J.; Hiebert, Randy International Journal of Greenhouse Gas Control, Vol. 3, Issue 1, p. 90-99 https://doi.org/10.1016/j.ijggc.2008.05.002	journal	January 2009
Microbially mediated plugging of porous media and the impact of differing injection strategies Tobler, Dominique J.; Maclachlan, Erica; Phoenix, Vernon R. Ecological Engineering, Vol. 42 https://doi.org/10.1016/j.ecoleng.2012.02.027	journal	May 2012
Urease activity in microbiologically-induced calcite precipitation Bachmeier, Keri L.; Williams, Amy E.; Warmington, John R. Journal of Biotechnology, Vol. 93, Issue 2 https://doi.org/10.1016/S0168-1656(01)00393-5	journal	February 2002
Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM) Koley, D.; Bard, A. J. Proceedings of the National Academy of Sciences, Vol. 107, Issue 39 https://doi.org/10.1073/pnas.1011614107	journal	September 2010
Precipitation of carbonate minerals by microorganisms: Implications for silicate weathering and the global carbon dioxide budget Ferris, F. G.; Wiese, R. G.; Fyfe, W. S. Geomicrobiology Journal, Vol. 12, Issue 1 https://doi.org/10.1080/01490459409377966	journal	January 1994
Application of bacteria as self-healing agent for the development of sustainable concrete Jonkers, Henk M.; Thijssen, Arjan; Muyzer, Gerard Ecological Engineering, Vol. 36, Issue 2 https://doi.org/10.1016/j.ecoleng.2008.12.036	journal	February 2010
Biocalcification by Bacillus pasteurii urease: a novel application Sarda, Deepak; Choonia, Huzaifa S.; Sarode, D. D. Journal of Industrial Microbiology & Biotechnology, Vol. 36, Issue 8 https://doi.org/10.1007/s10295-009-0581-4	journal	May 2009
A dual electrochemical microsensor for simultaneous imaging of oxygen and pH over the rat kidney surface Ha, Yejin; Myung, Dongshin; Shim, Jun Ho The Analyst, Vol. 138, Issue 18 https://doi.org/10.1039/c3an00878a	journal	January 2013
Discovery of a biofilm electrocline using real-time 3D metabolite analysis Koley, D.; Ramsey, M. M.; Bard, A. J. Proceedings of the National Academy of Sciences, Vol. 108, Issue 50 https://doi.org/10.1073/pnas.1117298108	journal	November 2011
Constant-Distance Mode Scanning Potentiometry. 1. Visualization of Calcium Carbonate Dissolution in Aqueous Solution Etienne, Mathieu; Schulte, Albert; Mann, Stefan Analytical Chemistry, Vol. 76, Issue 13 https://doi.org/10.1021/ac0349227	journal	July 2004
Theory of Microbial Carbonate Precipitation and Its Application in Restoration of Cement-based Materials Defects Qian, Chunxiang; Wang, Ruixing; Cheng, Liang Chinese Journal of Chemistry, Vol. 28, Issue 5 https://doi.org/10.1002/cjoc.201090156	journal	May 2010
Effect of Interparticle Electrostatic Double Layer Interactions on Permeate Flux Decline in Crossflow Membrane Filtration of Colloidal Suspensions: An Experimental Investigation Faibish, Ron S.; Elimelech, Menachem; Cohen, Yoram Journal of Colloid and Interface Science, Vol. 204, Issue 1 https://doi.org/10.1006/jcis.1998.5563	journal	August 1998
Upscaling and its application in numerical simulation of long-term CO ₂ storage Akber Hassan, Wasim A.; Jiang, Xi Greenhouse Gases: Science and Technology, Vol. 2, Issue 6 https://doi.org/10.1002/ghg.1306	journal	October 2012
Geochemical alteration of simulated wellbores of CO2 injection sites within the Illinois and Pasco Basins Verba, Circe; O’Connor, William; Rush, Gilbert International Journal of Greenhouse Gas Control, Vol. 23 https://doi.org/10.1016/j.ijggc.2014.01.015	journal	April 2014
Reservoir characterization of the Mt. Simon Sandstone, Illinois Basin, USA Frailey, Scott M.; Damico, James; Leetaru, Hannes E. Energy Procedia, Vol. 4 https://doi.org/10.1016/j.egypro.2011.02.534	journal	January 2011
Optimum conditions for microbial carbonate precipitation Okwadha, George D. O.; Li, Jin Chemosphere, Vol. 81, Issue 9 https://doi.org/10.1016/j.chemosphere.2010.09.066	journal	November 2010
Inhibition of the MRP1-mediated transport of the menadione-glutathione conjugate (thiodione) in HeLa cells as studied by SECM Koley, D.; Bard, A. J. Proceedings of the National Academy of Sciences, Vol. 109, Issue 29 https://doi.org/10.1073/pnas.1201555109	journal	June 2012
Mathematical model of biofilm induced calcite precipitation Zhang, T.; Klapper, I. Water Science and Technology, Vol. 61, Issue 11 https://doi.org/10.2166/wst.2010.064	journal	June 2010
Use of bacteria to repair cracks in concrete Van Tittelboom, Kim; De Belie, Nele; De Muynck, Willem Cement and Concrete Research, Vol. 40, Issue 1 https://doi.org/10.1016/j.cemconres.2009.08.025	journal	January 2010
Single-cell migration as studied by scanning electrochemical microscopy Ummadi, J. Ganesh; Joshi, Vrushali S.; Gupta, Priya R. Analytical Methods, Vol. 7, Issue 20 https://doi.org/10.1039/C5AY01944C	journal	January 2015
Solid-state pH nanoelectrode based on polyaniline thin film electrodeposited onto ion-beam etched carbon fiber Zhang, Xueji; Ogorevc, Božidar; Wang, Joseph Analytica Chimica Acta, Vol. 452, Issue 1 https://doi.org/10.1016/S0003-2670(01)01435-0	journal	January 2002
Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone De Muynck, Willem; Verbeken, Kim; De Belie, Nele Ecological Engineering, Vol. 36, Issue 2 https://doi.org/10.1016/j.ecoleng.2009.03.025	journal	February 2010
Solid-state pH ultramicrosensor based on a tungstic oxide film fabricated on a tungsten nanoelectrode and its application to the study of endothelial cells Yamamoto, Katsunobu; Shi, Guoyue; Zhou, Tianshu Analytica Chimica Acta, Vol. 480, Issue 1 https://doi.org/10.1016/S0003-2670(02)01602-1	journal	March 2003
Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species Dick, Jan; De Windt, Wim; De Graef, Bernard Biodegradation, Vol. 17, Issue 4 https://doi.org/10.1007/s10532-005-9006-x	journal	February 2006
A new method for controlling leaching through permeable channels Gollapudi, U. K.; Knutson, C. L.; Bang, S. S. Chemosphere, Vol. 30, Issue 4 https://doi.org/10.1016/0045-6535(94)00435-W	journal	February 1995
The kinetics of calcite precipitation from a high salinity water Zhang, Yuping; Dawe, Richard Applied Geochemistry, Vol. 13, Issue 2 https://doi.org/10.1016/S0883-2927(97)00061-9	journal	March 1998
Mathematical model of the effect of electrodiffusion on biomineralization Zhang, Tianyu; Klapper, Isaac International Journal of Non-Linear Mechanics, Vol. 46, Issue 4 https://doi.org/10.1016/j.ijnonlinmec.2010.12.008	journal	May 2011
Subscribed Content Calcium Carbonate Precipitation by Ureolytic Subsurface Bacteria Fujita, Y.; Ferris, F. G.; Lawson, R. D. Geomicrobiology Journal, Vol. 17, Issue 4 https://doi.org/10.1080/782198884	journal	October 2000
Evaluation of data from the literature on the transport and survival of Escherichia coli and thermotolerant coliforms in aquifers under saturated conditions Foppen, J. W. A.; Schijven, J. F. Water Research, Vol. 40, Issue 3 https://doi.org/10.1016/j.watres.2005.11.018	journal	February 2006
Microbially Enhanced Carbon Capture and Storage by Mineral-Trapping and Solubility-Trapping Mitchell, Andrew C.; Dideriksen, Knud; Spangler, Lee H. Environmental Science & Technology, Vol. 44, Issue 13 https://doi.org/10.1021/es903270w	journal	July 2010
Microbial CaCO3 mineral formation and stability in an experimentally simulated high pressure saline aquifer with supercritical CO2 Mitchell, Andrew C.; Phillips, Adrienne; Schultz, Logan International Journal of Greenhouse Gas Control, Vol. 15 https://doi.org/10.1016/j.ijggc.2013.02.001	journal	July 2013
Microbial Concrete: Way to Enhance the Durability of Building Structures Achal, Varenyam; Mukherjee, Abhijit; Reddy, M. Sudhakara Journal of Materials in Civil Engineering, Vol. 23, Issue 6 https://doi.org/10.1061/(ASCE)MT.1943-5533.0000159	journal	June 2011
Scanning Electrochemical Microscopy Amemiya, Shigeru; Bard, Allen J.; Fan, Fu-Ren F. Annual Review of Analytical Chemistry, Vol. 1, Issue 1 https://doi.org/10.1146/annurev.anchem.1.031207.112938	journal	July 2008
Microbiological precipitation of CaCO3 Stocks-Fischer, Shannon; Galinat, Johnna K.; Bang, Sookie S. Soil Biology and Biochemistry, Vol. 31, Issue 11 https://doi.org/10.1016/S0038-0717(99)00082-6	journal	October 1999
Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20°C in artificial groundwater Ferris, F. G.; Phoenix, V.; Fujita, Y. Geochimica et Cosmochimica Acta, Vol. 68, Issue 8 https://doi.org/10.1016/S0016-7037(03)00503-9	journal	April 2004
Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production Achal, V.; Mukherjee, A.; Basu, P. C. Journal of Industrial Microbiology & Biotechnology, Vol. 36, Issue 7 https://doi.org/10.1007/s10295-009-0578-z	journal	May 2009
Experimental and numerical modeling of bacterially induced pH increase and calcite precipitation in saline aquifers Dupraz, Sébastien; Parmentier, Marc; Ménez, Bénédicte Chemical Geology, Vol. 265, Issue 1-2 https://doi.org/10.1016/j.chemgeo.2009.05.003	journal	July 2009
Microbial carbonate precipitation in construction materials: A review De Muynck, Willem; De Belie, Nele; Verstraete, Willy Ecological Engineering, Vol. 36, Issue 2 https://doi.org/10.1016/j.ecoleng.2009.02.006	journal	February 2010
Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy Liu, X.; Ramsey, M. M.; Chen, X. Proceedings of the National Academy of Sciences, Vol. 108, Issue 7 https://doi.org/10.1073/pnas.1018391108	journal	January 2011

Similar Records

Kinetics of calcite precipitation by ureolytic bacteria under aerobic and anaerobic conditions

Journal Article · Thu May 23 00:00:00 EDT 2019 · Biogeosciences (Online) · OSTI ID:1263586

Mitchell, Andrew C.; Espinosa-Ortiz, Erika J.; Parks, Stacy L.; +3 more

Calcite Precipitation and Trace Metal Partitioning in Groundwater and the Vadose Zone: Remediation of Strontium-90 and Other Divalent Metals and Radionuclides in Arid Western Environments

Technical Report · Sat Apr 12 00:00:00 EDT 2003 · OSTI ID:1263586

Ferris, F Grant

Microbial Carbonation of Monocalcium Silicate

Journal Article · Wed Apr 06 00:00:00 EDT 2022 · ACS Omega · OSTI ID:1263586

Guzman, Michael S.; Iyer, Jaisree; Kim, Paul; +6 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Scanning electrochemical microscopy
Sporosarcina pasteurii
biocement
biofilm
microscopy

Title: Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

Citation Formats

References (40)

Similar Records

Related Subjects