Initiation of immune tolerance–controlled HIV gp41 neutralizing B cell lineages

Zhang, Ruijun; Verkoczy, Laurent; Wiehe, Kevin; Alam, S. Munir; Nicely, Nathan I.; Santra, Sampa; Bradley, Todd; Pemble, IV, Charles W.; Zhang, Jinsong; Gao, Feng; Montefiori, David C.; Bouton-Verville, Hilary; Kelsoe, Garnett; Larimore, Kevin; Greenberg, Phillip D.; Parks, Robert; Foulger, Andrew; Peel, Jessica N.; Luo, Kan; Lu, Xiaozhi; Trama, Ashley M.; Vandergrift, Nathan; Tomaras, Georgia D.; Kepler, Thomas B.; Moody, M. Anthony; Liao, Hua-Xin; Haynes, Barton F.

doi:10.1126/scitranslmed.aaf0618

Title: Initiation of immune tolerance–controlled HIV gp41 neutralizing B cell lineages

Journal Article · Wed Apr 27 00:00:00 EDT 2016 · Science Translational Medicine

DOI:https://doi.org/10.1126/scitranslmed.aaf0618· OSTI ID:1257467

Zhang, Ruijun ^[1]; Verkoczy, Laurent ^[1]; Wiehe, Kevin ^[1]; Alam, S. Munir ^[1]; Nicely, Nathan I. ^[1]; Santra, Sampa ^[2]; Bradley, Todd ^[1]; Pemble, IV, Charles W. ^[1]; Zhang, Jinsong ^[1]; Gao, Feng ^[1]; Montefiori, David C. ^[1]; Bouton-Verville, Hilary ^[1]; Kelsoe, Garnett ^[1]; Larimore, Kevin ^[3]; Greenberg, Phillip D. ^[4]; Parks, Robert ^[1]; Foulger, Andrew ^[1]; Peel, Jessica N. ^[1]; Luo, Kan ^[1]; Lu, Xiaozhi ^[1] more »

Duke Univ. School of Medicine, Durham, NC (United States)
Beth Israel Deaconess Medical Center, Boston, MA (United States)
Univ. of Washington, Seattle, WA (United States); BioMarin Pharmaceutical Inc., Novato, CA (United States)
Univ. of Washington, Seattle, WA (United States)
Boston Univ. School of Medicine, Boston, MA (United States)

Development of an HIV vaccine is a global priority. A major roadblock to a vaccine is an inability to induce protective broadly neutralizing antibodies (bnAbs). HIV gp41 bnAbs have characteristics that predispose them to be controlled by tolerance. We used gp41 2F5 bnAb germline knock-in mice and macaques vaccinated with immunogens reactive with germline precursors to activate neutralizing antibodies. In germline knock-in mice, bnAb precursors were deleted, with remaining anergic B cells capable of being activated by germline-binding immunogens to make gp41-reactive immunoglobulin M (IgM). Immunized macaques made B cell clonal lineages targeted to the 2F5 bnAb epitope, but 2F5-like antibodies were either deleted or did not attain sufficient affinity for gp41-lipid complexes to achieve the neutralization potency of 2F5. Structural analysis of members of a vaccine-induced antibody lineage revealed that heavy chain complementarity-determining region 3 (HCDR3) hydrophobicity was important for neutralization. Furthermore, gp41 bnAbs are controlled by immune tolerance, requiring vaccination strategies to transiently circumvent tolerance controls.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; National Institutes of Health (NIH); National Inst. of Allergy and Infectious Diseases

Grant/Contract Number:: W-31-109-Eng-38; CHAVI-ID; UMI-AI100645

OSTI ID:: 1257467

Journal Information:: Science Translational Medicine, Vol. 8, Issue 336; ISSN 1946-6234

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 52 works

Citation information provided by
Web of Science

References (70)

Structural Analysis of Human and Macaque mAbs 2909 and 2.5B: Implications for the Configuration of the Quaternary Neutralizing Epitope of HIV-1 gp120 Spurrier, Brett; Sampson, Jared M.; Totrov, Maxim Structure, Vol. 19, Issue 5 https://doi.org/10.1016/j.str.2011.02.012	journal	May 2011
Structure and Mechanistic Analysis of the Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5 in Complex with Its gp41 Epitope Ofek, G.; Tang, M.; Sambor, A. Journal of Virology, Vol. 78, Issue 19 https://doi.org/10.1128/JVI.78.19.10724-10737.2004	journal	September 2004
Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice Dosenovic, Pia; von Boehmer, Lotta; Escolano, Amelia Cell, Vol. 161, Issue 7 https://doi.org/10.1016/j.cell.2015.06.003	journal	June 2015
Utilization of Immunoglobulin G Fc Receptors by Human Immunodeficiency Virus Type 1: a Specific Role for Antibodies against the Membrane-Proximal External Region of gp41 Perez, Lautaro G.; Costa, Matthew R.; Todd, Christopher A. Journal of Virology, Vol. 83, Issue 15 https://doi.org/10.1128/JVI.00656-09	journal	May 2009
Distinct conformational states of HIV-1 gp41 are recognized by neutralizing and non-neutralizing antibodies Frey, Gary; Chen, Jia; Rits-Volloch, Sophia Nature Structural & Molecular Biology, Vol. 17, Issue 12 https://doi.org/10.1038/nsmb.1950	journal	November 2010
T Follicular Helper Cell Differentiation, Function, and Roles in Disease Crotty, Shane Immunity, Vol. 41, Issue 4 https://doi.org/10.1016/j.immuni.2014.10.004	journal	October 2014
Deficiency in CD22, a B Cell–specific Inhibitory Receptor, Is Sufficient to Predispose to Development of High Affinity Autoantibodies O'Keefe, Theresa L.; Williams, Gareth T.; Batista, Facundo D. Journal of Experimental Medicine, Vol. 189, Issue 8 https://doi.org/10.1084/jem.189.8.1307	journal	April 1999
Stapled HIV-1 peptides recapitulate antigenic structures and engage broadly neutralizing antibodies Bird, Gregory H.; Irimia, Adriana; Ofek, Gilad Nature Structural & Molecular Biology, Vol. 21, Issue 12 https://doi.org/10.1038/nsmb.2922	journal	November 2014
Functional, Non-Clonal IgMa-Restricted B Cell Receptor Interactions with the HIV-1 Envelope gp41 Membrane Proximal External Region Verkoczy, Laurent; Moody, M. Anthony; Holl, T. Matt PLoS ONE, Vol. 4, Issue 10 https://doi.org/10.1371/journal.pone.0007215	journal	October 2009
Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding Appleton, Brent A.; Wu, Ping; Maloney, Janice The EMBO Journal, Vol. 26, Issue 23 https://doi.org/10.1038/sj.emboj.7601906	journal	November 2007
HIV-1 Broadly Neutralizing Antibody Extracts Its Epitope from a Kinked gp41 Ectodomain Region on the Viral Membrane Sun, Zhen-Yu J.; Oh, Kyoung Joon; Kim, Mikyung Immunity, Vol. 28, Issue 1 https://doi.org/10.1016/j.immuni.2007.11.018	journal	January 2008
Common Tolerance Mechanisms, but Distinct Cross-Reactivities Associated with gp41 and Lipids, Limit Production of HIV-1 Broad Neutralizing Antibodies 2F5 and 4E10 Chen, Yao; Zhang, Jinsong; Hwang, Kwan-Ki The Journal of Immunology, Vol. 191, Issue 3 https://doi.org/10.4049/jimmunol.1300770	journal	July 2013
High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies Liao, Hua-Xin; Levesque, Marc C.; Nagel, Ashleigh Journal of Virological Methods, Vol. 158, Issue 1-2 https://doi.org/10.1016/j.jviromet.2009.02.014	journal	June 2009
Redemption of autoreactive B cells Haynes, B. F.; Verkoczy, L.; Kelsoe, G. Proceedings of the National Academy of Sciences, Vol. 111, Issue 25 https://doi.org/10.1073/pnas.1407877111	journal	June 2014
Structure-guided Alterations of the gp41-directed HIV-1 Broadly Neutralizing Antibody 2F5 Reveal New Properties Regarding its Neutralizing Function Guenaga, Javier; Wyatt, Richard T. PLoS Pathogens, Vol. 8, Issue 7 https://doi.org/10.1371/journal.ppat.1002806	journal	July 2012
Isolation of a Human Anti-HIV gp41 Membrane Proximal Region Neutralizing Antibody by Antigen-Specific Single B Cell Sorting Morris, Lynn; Chen, Xi; Alam, Munir PLoS ONE, Vol. 6, Issue 9 https://doi.org/10.1371/journal.pone.0023532	journal	September 2011
Antibody responses to envelope glycoproteins in HIV-1 infection Burton, Dennis R.; Mascola, John R. Nature Immunology, Vol. 16, Issue 6 https://doi.org/10.1038/ni.3158	journal	May 2015
Redemption of autoantibodies on anergic B cells by variable-region glycosylation and mutation away from self-reactivity Sabouri, Z.; Schofield, P.; Horikawa, K. Proceedings of the National Academy of Sciences, Vol. 111, Issue 25 https://doi.org/10.1073/pnas.1406974111	journal	May 2014
The Role of Antibody Polyspecificity and Lipid Reactivity in Binding of Broadly Neutralizing Anti-HIV-1 Envelope Human Monoclonal Antibodies 2F5 and 4E10 to Glycoprotein 41 Membrane Proximal Envelope Epitopes Alam, S. Munir; McAdams, Mildred; Boren, David The Journal of Immunology, Vol. 178, Issue 7 https://doi.org/10.4049/jimmunol.178.7.4424	journal	March 2007
Cardiolipin Polyspecific Autoreactivity in Two Broadly Neutralizing HIV-1 Antibodies Haynes, B. F. Science, Vol. 308, Issue 5730 https://doi.org/10.1126/science.1111781	journal	June 2005
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Initial B-Cell Responses to Transmitted Human Immunodeficiency Virus Type 1: Virion-Binding Immunoglobulin M (IgM) and IgG Antibodies Followed by Plasma Anti-gp41 Antibodies with Ineffective Control of Initial Viremia Tomaras, G. D.; Yates, N. L.; Liu, P. Journal of Virology, Vol. 82, Issue 24, p. 12449-12463 https://doi.org/10.1128/JVI.01708-08	journal	October 2008
Kinetic and affinity limits on antibodies produced during immune responses. Foote, J.; Eisen, H. N. Proceedings of the National Academy of Sciences, Vol. 92, Issue 5 https://doi.org/10.1073/pnas.92.5.1254	journal	February 1995
NMRPipe: A multidimensional spectral processing system based on UNIX pipes Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW. Journal of Biomolecular NMR, Vol. 6, Issue 3 https://doi.org/10.1007/BF00197809	journal	November 1995
Early high-affinity neutralizing anti-viral IgG responses without further overall improvements of affinity. Roost, H. P.; Bachmann, M. F.; Haag, A. Proceedings of the National Academy of Sciences, Vol. 92, Issue 5 https://doi.org/10.1073/pnas.92.5.1257	journal	February 1995
Reconstructing a B-cell clonal lineage. I. Statistical inference of unobserved ancestors Kepler, Thomas B. F1000Research, Vol. 2 https://doi.org/10.12688/f1000research.2-103.v1	journal	January 2013
Human Circulating PD-1+CXCR3−CXCR5+ Memory Tfh Cells Are Highly Functional and Correlate with Broadly Neutralizing HIV Antibody Responses Locci, Michela; Havenar-Daughton, Colin; Landais, Elise Immunity, Vol. 39, Issue 4 https://doi.org/10.1016/j.immuni.2013.08.031	journal	October 2013
Immunoglobulin heavy chain expression shapes the B cell receptor repertoire in human B cell development Meffre, Eric; Milili, Michèle; Blanco-Betancourt, Carla Journal of Clinical Investigation, Vol. 108, Issue 6 https://doi.org/10.1172/JCI200113051	journal	September 2001
Differential Reactivity of Germ Line Allelic Variants of a Broadly Neutralizing HIV-1 Antibody to a gp41 Fusion Intermediate Conformation Alam, S. M.; Liao, H. -X.; Dennison, S. M. Journal of Virology, Vol. 85, Issue 22 https://doi.org/10.1128/JVI.05680-11	journal	September 2011
Polyreactivity and Autoreactivity among HIV-1 Antibodies Liu, Mengfei; Yang, Guang; Wiehe, Kevin Journal of Virology, Vol. 89, Issue 1 https://doi.org/10.1128/JVI.02378-14	journal	October 2014
Structural Basis for HIV-1 Neutralization by 2F5-Like Antibodies m66 and m66.6 Ofek, G.; Zirkle, B.; Yang, Y. Journal of Virology, Vol. 88, Issue 5 https://doi.org/10.1128/JVI.02837-13	journal	December 2013
Exposure of the Membrane-Proximal External Region of HIV-1 gp41 in the Course of HIV-1 Envelope Glycoprotein-Mediated Fusion ^† Dimitrov, Antony S.; Jacobs, Amy; Finnegan, Catherine M. Biochemistry, Vol. 46, Issue 5 https://doi.org/10.1021/bi062245f	journal	February 2007
Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution Shen, X.; Dennison, S. M.; Liu, P. Proceedings of the National Academy of Sciences, Vol. 107, Issue 13 https://doi.org/10.1073/pnas.0912381107	journal	March 2010
Induction of self-tolerance in mature peripheral B lymphocytes Goodnow, Christopher C.; Crosbie, Jeffrey; Jorgensen, Helle Nature, Vol. 342, Issue 6248 https://doi.org/10.1038/342385a0	journal	November 1989
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1 https://doi.org/10.1107/S090744490705024X	journal	December 2007
Induction of Antibodies in Rhesus Macaques That Recognize a Fusion-Intermediate Conformation of HIV-1 gp41 Dennison, S. Moses; Sutherland, Laura L.; Jaeger, Frederick H. PLoS ONE, Vol. 6, Issue 11 https://doi.org/10.1371/journal.pone.0027824	journal	November 2011
HIV-1 neutralizing antibodies: understanding nature's pathways Mascola, John R.; Haynes, Barton F. Immunological Reviews, Vol. 254, Issue 1 https://doi.org/10.1111/imr.12075	journal	June 2013
Mosaic vaccines elicit CD8⁺ T lymphocyte responses that confer enhanced immune coverage of diverse HIV strains in monkeys Santra, Sampa; Liao, Hua-Xin; Zhang, Ruijin Nature Medicine, Vol. 16, Issue 3, p. 324-328 https://doi.org/10.1038/nm.2108	journal	February 2010
Mechanism of HIV-1 Neutralization by Antibodies Targeting a Membrane-Proximal Region of gp41 Chen, J.; Frey, G.; Peng, H. Journal of Virology, Vol. 88, Issue 2 https://doi.org/10.1128/JVI.02664-13	journal	November 2013
Rescue of HIV-1 Broad Neutralizing Antibody-Expressing B Cells in 2F5 V _H × V _L Knockin Mice Reveals Multiple Tolerance Controls Verkoczy, Laurent; Chen, Yao; Bouton-Verville, Hilary The Journal of Immunology, Vol. 187, Issue 7 https://doi.org/10.4049/jimmunol.1101633	journal	September 2011
Nonneutralizing HIV-1 gp41 Envelope Cluster II Human Monoclonal Antibodies Show Polyreactivity for Binding to Phospholipids and Protein Autoantigens Dennison, S. M.; Anasti, K.; Scearce, R. M. Journal of Virology, Vol. 85, Issue 3 https://doi.org/10.1128/JVI.01680-10	journal	November 2010
Vaccine Induction of Antibodies against a Structurally Heterogeneous Site of Immune Pressure within HIV-1 Envelope Protein Variable Regions 1 and 2 Liao, Hua-Xin; Bonsignori, Mattia; Alam, S. Munir Immunity, Vol. 38, Issue 1 https://doi.org/10.1016/j.immuni.2012.11.011	journal	January 2013
Kinetics of protein-protein association explained by Brownian dynamics computer simulation. Northrup, S. H.; Erickson, H. P. Proceedings of the National Academy of Sciences, Vol. 89, Issue 8 https://doi.org/10.1073/pnas.89.8.3338	journal	April 1992
Antibody Light-Chain-Restricted Recognition of the Site of Immune Pressure in the RV144 HIV-1 Vaccine Trial Is Phylogenetically Conserved Wiehe, Kevin; Easterhoff, David; Luo, Kan Immunity, Vol. 41, Issue 6 https://doi.org/10.1016/j.immuni.2014.11.014	journal	December 2014
Induction of HIV-1 Broad Neutralizing Antibodies in 2F5 Knock-in Mice: Selection against Membrane Proximal External Region–Associated Autoreactivity Limits T-Dependent Responses Verkoczy, Laurent; Chen, Yao; Zhang, Jinsong The Journal of Immunology, Vol. 191, Issue 5 https://doi.org/10.4049/jimmunol.1300971	journal	August 2013
Modulation of Nonneutralizing HIV-1 gp41 Responses by an MHC-Restricted T _H Epitope Overlapping Those of Membrane Proximal External Region Broadly Neutralizing Antibodies Zhang, Jinsong; Alam, S. Munir; Bouton-Verville, Hilary The Journal of Immunology, Vol. 192, Issue 4 https://doi.org/10.4049/jimmunol.1302511	journal	January 2014
Envelope Deglycosylation Enhances Antigenicity of HIV-1 gp41 Epitopes for Both Broad Neutralizing Antibodies and Their Unmutated Ancestor Antibodies Ma, Ben-Jiang; Alam, S. Munir; Go, Eden P. PLoS Pathogens, Vol. 7, Issue 9 https://doi.org/10.1371/journal.ppat.1002200	journal	September 2011
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing Nature, Vol. 496, Issue 7446 https://doi.org/10.1038/nature12053	journal	April 2013
Human Immunodeficiency Virus Type 1 env Clones from Acute and Early Subtype B Infections for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies Li, M.; Gao, F.; Mascola, J. R. Journal of Virology, Vol. 79, Issue 16 https://doi.org/10.1128/JVI.79.16.10108-10125.2005	journal	July 2005
HIV-1 gp41 envelope IgA is frequently elicited after transmission but has an initial short response half-life Yates, N. L.; Stacey, A. R.; Nolen, T. L. Mucosal Immunology, Vol. 6, Issue 4 https://doi.org/10.1038/mi.2012.107	journal	January 2013
HIV-1 neutralizing antibodies induced by native-like envelope trimers Sanders, R. W.; van Gils, M. J.; Derking, R. Science, Vol. 349, Issue 6244 https://doi.org/10.1126/science.aac4223	journal	June 2015
Sequestering of the Prehairpin Intermediate of gp41 by Peptide N36Mut(e,g) Potentiates the Human Immunodeficiency Virus Type 1 Neutralizing Activity of Monoclonal Antibodies Directed against the N-Terminal Helical Repeat of gp41 Gustchina, Elena; Bewley, Carole A.; Clore, G. Marius Journal of Virology, Vol. 82, Issue 20 https://doi.org/10.1128/JVI.01050-08	journal	July 2008
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies McGuire, Andrew T.; Hoot, Sam; Dreyer, Anita M. The Journal of Experimental Medicine, Vol. 210, Issue 4 https://doi.org/10.1084/jem.20122824	journal	March 2013
Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies Yang, Guang; Holl, T. Matt; Liu, Yang The Journal of Experimental Medicine, Vol. 210, Issue 2 https://doi.org/10.1084/jem.20121977	journal	January 2013
Protein Design of an HIV-1 Entry Inhibitor Root, M. J. Science, Vol. 291, Issue 5505 https://doi.org/10.1126/science.1057453	journal	January 2001
Role of HIV membrane in neutralization by two broadly neutralizing antibodies Alam, S. M.; Morelli, M.; Dennison, S. M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 48 https://doi.org/10.1073/pnas.0908713106	journal	November 2009
Structure validation by Cα geometry: ϕ,ψ and Cβ deviation Lovell, Simon C.; Davis, Ian W.; Arendall, W. Bryan Proteins: Structure, Function, and Bioinformatics, Vol. 50, Issue 3 https://doi.org/10.1002/prot.10286	journal	January 2003
Solvent content of protein crystals Matthews, B. W. Journal of Molecular Biology, Vol. 33, Issue 2 https://doi.org/10.1016/0022-2836(68)90205-2	journal	April 1968
Stable Docking of Neutralizing Human Immunodeficiency Virus Type 1 gp41 Membrane-Proximal External Region Monoclonal Antibodies 2F5 and 4E10 Is Dependent on the Membrane Immersion Depth of Their Epitope Regions Dennison, S. M.; Stewart, S. M.; Stempel, K. C. Journal of Virology, Vol. 83, Issue 19 https://doi.org/10.1128/JVI.00571-09	journal	July 2009
Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation Kepler, Thomas B.; Munshaw, Supriya; Wiehe, Kevin Frontiers in Immunology, Vol. 5 https://doi.org/10.3389/fimmu.2014.00170	journal	April 2014
Diversion of HIV-1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies Williams, W. B.; Liao, H. -X.; Moody, M. A. Science, Vol. 349, Issue 6249 https://doi.org/10.1126/science.aab1253	journal	July 2015
MPEx: A tool for exploring membrane proteins Snider, Craig; Jayasinghe, Sajith; Hristova, Kalina Protein Science, Vol. 18, Issue 12 https://doi.org/10.1002/pro.256	journal	December 2009
Antibody mechanics on a membrane-bound HIV segment essential for GP41-targeted viral neutralization Kim, Mikyung; Sun, Zhen-Yu J.; Rand, Kasper D. Nature Structural & Molecular Biology, Vol. 18, Issue 11 https://doi.org/10.1038/nsmb.2154	journal	October 2011
CD4 Ligation on Human Blood Monocytes Triggers Macrophage Differentiation and Enhances HIV Infection Zhen, A.; Krutzik, S. R.; Levin, B. R. Journal of Virology, Vol. 88, Issue 17 https://doi.org/10.1128/JVI.00616-14	journal	June 2014
Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance Verkoczy, L.; Diaz, M.; Holl, T. M. Proceedings of the National Academy of Sciences, Vol. 107, Issue 1 https://doi.org/10.1073/pnas.0912914107	journal	December 2009
Experimentally determined hydrophobicity scale for proteins at membrane interfaces Wimley, William C.; White, Stephen H. Nature Structural & Molecular Biology, Vol. 3, Issue 10 https://doi.org/10.1038/nsb1096-842	journal	October 1996
HIV-1 gp120 Vaccine Induces Affinity Maturation in both New and Persistent Antibody Clonal Lineages Moody, M. A.; Yates, N. L.; Amos, J. D. Journal of Virology, Vol. 86, Issue 14 https://doi.org/10.1128/JVI.00426-12	journal	May 2012
Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen Jardine, J. G.; Ota, T.; Sok, D. Science, Vol. 349, Issue 6244 https://doi.org/10.1126/science.aac5894	journal	June 2015
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997

Cited By (27)

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation Saunders, Kevin O.; Wiehe, Kevin; Tian, Ming Science, Vol. 366, Issue 6470 https://doi.org/10.1126/science.aay7199	journal	December 2019
Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor The Journal of Experimental Medicine, Vol. 216, Issue 10 https://doi.org/10.1084/jem.20190164	journal	July 2019
Strategies for inducing effective neutralizing antibody responses against HIV-1 del Moral-Sánchez, Iván; Sliepen, Kwinten Expert Review of Vaccines, Vol. 18, Issue 11 https://doi.org/10.1080/14760584.2019.1690458	journal	November 2019
Clonal redemption and clonal anergy as mechanisms to balance B cell tolerance and immunity Burnett, Deborah L.; Reed, Joanne H.; Christ, Daniel Immunological Reviews, Vol. 292, Issue 1 https://doi.org/10.1111/imr.12808	journal	September 2019
Minding the gap: The impact of B‐cell tolerance on the microbial antibody repertoire Finney, Joel; Watanabe, Akiko; Kelsoe, Garnett Immunological Reviews, Vol. 292, Issue 1 https://doi.org/10.1111/imr.12805	journal	September 2019
Distinct, IgG1-driven antibody response landscapes demarcate individuals with broadly HIV-1 neutralizing activity Kadelka, Claus; Liechti, Thomas; Ebner, Hanna Journal of Experimental Medicine, Vol. 215, Issue 6 https://doi.org/10.1084/jem.20180246	journal	May 2018
Germline-targeting immunogens Stamatatos, Leonidas; Pancera, Marie; McGuire, Andrew T. Immunological Reviews, Vol. 275, Issue 1 https://doi.org/10.1111/imr.12483	journal	January 2017
Breaching peripheral tolerance promotes the production of HIV-1–neutralizing antibodies Schroeder, Kristin M. S.; Agazio, Amanda; Strauch, Pamela J. Journal of Experimental Medicine, Vol. 214, Issue 8 https://doi.org/10.1084/jem.20161190	journal	July 2017
Cross-Reactivity to Kynureninase Tolerizes B Cells That Express the HIV-1 Broadly Neutralizing Antibody 2F5 Finney, Joel; Yang, Guang; Kuraoka, Masayuki The Journal of Immunology, Vol. 203, Issue 12 https://doi.org/10.4049/jimmunol.1900069	journal	November 2019
Widespread B cell perturbations in HIV-1 infection afflict naive and marginal zone B cells Liechti, Thomas; Kadelka, Claus; Braun, Dominique L. Journal of Experimental Medicine, Vol. 216, Issue 9 https://doi.org/10.1084/jem.20181124	journal	June 2019
Optimal Development of Mature B Cells Requires Recognition of Endogenous Antigens Noviski, Mark; Tan, Corey; Huizar, John The Journal of Immunology, Vol. 203, Issue 2 https://doi.org/10.4049/jimmunol.1900175	journal	June 2019
Progress toward active or passive HIV-1 vaccination Escolano, Amelia; Dosenovic, Pia; Nussenzweig, Michel C. The Journal of Experimental Medicine, Vol. 214, Issue 1 https://doi.org/10.1084/jem.20161765	journal	December 2016
The expanding array of HIV broadly neutralizing antibodies McCoy, Laura E. Retrovirology, Vol. 15, Issue 1 https://doi.org/10.1186/s12977-018-0453-y	journal	October 2018
Human Immunodeficiency Virus Proteins Mimic Human T Cell Receptors Inducing Cross-Reactive Antibodies Root-Bernstein, Robert International Journal of Molecular Sciences, Vol. 18, Issue 10 https://doi.org/10.3390/ijms18102091	journal	October 2017
Potent and broad HIV-neutralizing antibodies in memory B cells and plasma Williams, LaTonya D.; Ofek, Gilad; Schätzle, Sebastian Science Immunology, Vol. 2, Issue 7 https://doi.org/10.1126/sciimmunol.aal2200	journal	January 2017
Distinct, IgG1-driven antibody response landscapes demarcate individuals with broadly HIV-1 neutralizing activity Kadelka, Claus; Liechti, Thomas; Ebner, Hanna Rockefeller University Press https://doi.org/10.5167/uzh-151852	text	January 2018
Structure of the membrane proximal external region of HIV-1 envelope glycoprotein Fu, Qingshan; Shaik, Md Munan; Cai, Yongfei Proceedings of the National Academy of Sciences, Vol. 115, Issue 38 https://doi.org/10.1073/pnas.1807259115	journal	September 2018
Widespread B cell perturbations in HIV-1 infection afflict naive and marginal zone B cells Liechti, Thomas; Kadelka, Claus; Braun, Dominique L. Rockefeller University Press https://doi.org/10.5167/uzh-176092	text	January 2019
Distinct, IgG1-driven antibody response landscapes demarcate individuals with broadly HIV-1 neutralizing activity. Kadelka, Claus; Liechti, Thomas; Ebner, Hanna Rockefeller University Press https://doi.org/10.7892/boris.117015	text	January 2018
HIV-1 envelope glycan modifications that permit neutralization by germline-reverted VRC01-class broadly neutralizing antibodies LaBranche, Celia C.; McGuire, Andrew T.; Gray, Matthew D. PLOS Pathogens, Vol. 14, Issue 11 https://doi.org/10.1371/journal.ppat.1007431	journal	November 2018
Guiding the humoral response against HIV-1 toward a MPER adjacent region by immunization with a VLP-formulated antibody-selected envelope variant Beltran-Pavez, Carolina; Ferreira, Carolina B.; Merino-Mansilla, Alberto PLOS ONE, Vol. 13, Issue 12 https://doi.org/10.1371/journal.pone.0208345	journal	December 2018
Antibody-virus co-evolution in HIV infection: paths for HIV vaccine development Bonsignori, Mattia; Liao, Hua-Xin; Gao, Feng Immunological Reviews, Vol. 275, Issue 1 https://doi.org/10.1111/imr.12509	journal	January 2017
Host controls of HIV broadly neutralizing antibody development Kelsoe, Garnett; Haynes, Barton F. Immunological Reviews, Vol. 275, Issue 1 https://doi.org/10.1111/imr.12508	journal	January 2017
Clonal anergy of CD117+chB6+ B cell progenitors induced by avian leukosis virus subgroup J is associated with immunological tolerance He, Shuhai; Zheng, Gaoying; Zhou, Defang Retrovirology, Vol. 16, Issue 1 https://doi.org/10.1186/s12977-018-0463-9	journal	January 2019
Human Ig knockin mice to study the development and regulation of HIV-1 broadly neutralizing antibodies Verkoczy, Laurent; Alt, Frederick W.; Tian, Ming Immunological Reviews, Vol. 275, Issue 1 https://doi.org/10.1111/imr.12505	journal	January 2017
Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations Williams, Wilton B.; Zhang, Jinsong; Jiang, Chuancang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01336-3	journal	November 2017
Immunologic Insights on the Membrane Proximal External Region: A Major Human Immunodeficiency Virus Type-1 Vaccine Target Molinos-Albert, Luis M.; Clotet, Bonaventura; Blanco, Julià Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.01154	journal	September 2017

Similar Records

Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations

Journal Article · Thu Nov 23 00:00:00 EST 2017 · Nature Communications · OSTI ID:1257467

Williams, Wilton B.; Zhang, Jinsong; Jiang, Chuancang; +41 more

Potent and broad HIV-neutralizing antibodies in memory B cells and plasma

Journal Article · Fri Jan 27 00:00:00 EST 2017 · Science Immunology · OSTI ID:1257467

Williams, LaTonya D.; Ofek, Gilad; Schatzle, Sebastian; +38 more

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

Journal Article · Thu Sep 01 00:00:00 EDT 2016 · Immunity · OSTI ID:1257467

Steichen, Jon M.; Kulp, Daniel W.; Tokatlian, Talar; +26 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Initiation of immune tolerance–controlled HIV gp41 neutralizing B cell lineages

Citation Formats

References (70)

Cited By (27)

Similar Records

Related Subjects