Beyond the Western front: Targeted proteomics and organelle abundance profiling

Parsons, Harriet T.; Heazlewood, Joshua L.

doi:10.3389/fpls.2015.00301

Title: Beyond the Western front: Targeted proteomics and organelle abundance profiling

Journal Article · Tue May 05 00:00:00 EDT 2015 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2015.00301· OSTI ID:1257256

Parsons, Harriet T. ^[1]; Heazlewood, Joshua L. ^[2]

Univ. of Copenhagen, Frederiksberg (Denmark)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of Melbourne, Melbourne, VIC (Australia)

The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that employ mass spectrometry to enable both relative and absolute quantification of many hundreds of proteins in a single sample from a single analysis provides a mechanism to overcome the expensive impediment in having to develop antibodies in plant science. Here, we consider it an opportune moment to consider and better develop the adoption of multiple reaction monitoring (MRM)-based analyses in plant biochemistry.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1257256

Journal Information:: Frontiers in Plant Science, Vol. 6; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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Title: Beyond the Western front: Targeted proteomics and organelle abundance profiling

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