Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis

McCaig, William D.; Loving, Crystal L.; Hughes, Holly R.; Brockmeier, Susan L.; Charbit, Alain

doi:10.1371/journal.pone.0149132

Title: Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis

Journal Article · Tue Mar 01 00:00:00 EST 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0149132· OSTI ID:1252954

McCaig, William D. ^[1]; Loving, Crystal L. ^[1]; Hughes, Holly R. ^[1]; Brockmeier, Susan L. ^[1]; Charbit, Alain ^[2]

National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA (United States)
Univ. Paris Descartes, Paris (France)

Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Lastly, vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); Oak Ridge Associated Universities (ORAU), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-06OR23100

OSTI ID:: 1252954

Journal Information:: PLoS ONE, Vol. 11, Issue 3; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 38 works

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59 BASIC BIOLOGICAL SCIENCES
swine
outer membrane proteins
vaccines
vesicles
virulence factors
proteases
vaccination and immunization
serum proteins

Title: Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis

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