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Title: Metabolic potential of fatty acid oxidation and anaerobic respiration by abundant members of Thaumarchaeota and Thermoplasmata in deep anoxic peat

Journal Article · · The ISME Journal
 [1];  [2];  [3];  [1]
  1. Georgia Inst. of Technology, Atlanta, GA (United States)
  2. Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States); Marine Biological Lab., Woods Hole, MA (United States); Zhejiang Univ., Hangzhou (China)
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To probe the metabolic potential of abundant Archaea in boreal peats, we reconstructed two near-complete archaeal genomes, affiliated with Thaumarchaeota group 1.1c (bin Fn1, 8% abundance), which was a genomically unrepresented group, and Thermoplasmata (bin Bg1, 26% abundance), from metagenomic data acquired from deep anoxic peat layers. Each of the near-complete genomes encodes the potential to degrade long-chain fatty acids (LCFA) via β-oxidation. Fn1 has the potential to oxidize LCFA either by syntrophic interaction with methanogens or by coupling oxidation with anaerobic respiration using fumarate as a terminal electron acceptor (TEA). Fn1 is the first Thaumarchaeota genome without an identifiable carbon fixation pathway, indicating that this mesophilic phylum encompasses more diverse metabolisms than previously thought. Furthermore, we report genetic evidence suggestive of sulfite and/or organosulfonate reduction by Thermoplasmata Bg1. In deep peat, inorganic TEAs are often depleted to extremely low levels, yet the anaerobic respiration predicted for two abundant archaeal members suggests organic electron acceptors such as fumarate and organosulfonate (enriched in humic substances) may be important for respiration and C mineralization in peatlands.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
National Science Foundation (NSF); Alfred P. Sloan Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1247153
Journal Information:
The ISME Journal, Vol. 9, Issue 12; ISSN 1751-7362
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 49 works
Citation information provided by
Web of Science

References (23)

Hepatic Gluconeogenesis/Glycolysis: Regulation and Structure/Function Relationships of Substrate Cycle Enzymes journal July 1991
Southern Appalachian Peatlands Support High Archaeal Diversity journal January 2014
Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol journal March 2008
Microbial Community Structure and Activity Linked to Contrasting Biogeochemical Gradients in Bog and Fen Environments of the Glacial Lake Agassiz Peatland journal July 2012
High abundance of Crenarchaeota in a temperate acidic forest soil: Highly abundant Crenarchaeota in a forest soil journal June 2007
First description of giant Archaea (Thaumarchaeota) associated with putative bacterial ectosymbionts in a sulfidic marine habitat: Giant archaea associated with epibiotic sulfur oxidizing bacteria journal August 2010
A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring journal February 2008
Fumarate as terminal acceptor of phosphorylative electron transport journal October 1978
Energetics of syntrophic cooperation in methanogenic degradation. journal January 1997
Northern Peatlands: Role in the Carbon Cycle and Probable Responses to Climatic Warming journal May 1991
Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil journal April 2011
Thaumarchaeotes abundant in refinery nitrifying sludges express amoA but are not obligate autotrophic ammonia oxidizers journal September 2011
Microbial Community Stratification Linked to Utilization of Carbohydrates and Phosphorus Limitation in a Boreal Peatland at Marcell Experimental Forest, Minnesota, USA journal March 2014
Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO2 fixation journal May 2014
Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon from soil and a member of the archaeal phylum Thaumarchaeota journal June 2014
Isolation of an autotrophic ammonia-oxidizing marine archaeon journal September 2005
Controls on bacterial and archaeal community structure and greenhouse gas production in natural, mined, and restored Canadian peatlands journal January 2013
Global ecological patterns in uncultured Archaea journal October 2009
Niche specialization of novel Thaumarchaeota to oxic and hypoxic acidic geothermal springs of Yellowstone National Park journal November 2013
Biology of the Cold Adapted Archaeon, Methanococcoides b urtonii Determined by Proteomics Using Liquid Chromatography-Tandem Mass Spectrometry journal December 2004
Dissimilatory Sulfite Reductase (Desulfoviridin) of the Taurine-Degrading, Non-Sulfate-Reducing Bacterium Bilophila wadsworthia RZATAU Contains a Fused DsrB-DsrD Subunit journal March 2001
Sulfate-reducing microorganisms in wetlands – fameless actors in carbon cycling and climate change journal January 2012
The purification and characterization of an extremely thermostable alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus. journal November 1993

Cited By (17)

Plant Community and Nitrogen Deposition as Drivers of Alpha and Beta Diversities of Prokaryotes in Reconstructed Oil Sand Soils and Natural Boreal Forest Soils journal February 2017
Genome-resolved metagenomics analysis provides insights into the ecological role of Thaumarchaeota in the Amazon River and its plume journal January 2020
Phylogenomics suggests oxygen availability as a driving force in Thaumarchaeota evolution journal April 2019
Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta journal July 2019
Divergent national-scale trends of microbial and animal biodiversity revealed across diverse temperate soil ecosystems journal March 2019
The growing tree of Archaea: new perspectives on their diversity, evolution and ecology journal August 2017
Abundance and Co-Distribution of Widespread Marine Archaeal Lineages in Surface Sediments of Freshwater Water Bodies across the Iberian Peninsula journal May 2017
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters journal May 2016
Metal contaminations impact archaeal community composition, abundance and function in remote alpine lakes journal May 2018
Microbial diversity in European alpine permafrost and active layers journal January 2016
Archaea dominate the microbial community in an ecosystem with low-to-moderate temperature and extreme acidity journal January 2019
Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H2S Production: Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model journal November 2018
Mining traits for the enrichment and isolation of not-yet-cultured populations journal June 2019
Microbial diversity in European alpine permafrost and active layers text January 2016
Isolation and characterization of a thermophilic sulfur- and iron-reducing thaumarchaeote from a terrestrial acidic hot spring journal June 2019
Cultivation and Genomic Analysis of “Candidatus Nitrosocaldus islandicus,” an Obligately Thermophilic, Ammonia-Oxidizing Thaumarchaeon from a Hot Spring Biofilm in Graendalur Valley, Iceland journal February 2018
Bacterial community composition in a polyculture system of Rhopilema esculenta , Penaeus monodon and Ruditapes philippinarum journal January 2019

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