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Title: Biological production of organic compounds

Patent ·
OSTI ID:1246901
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Strains of cyanobacteria that produce high levels of alpha ketoglutarate (AKG) and pyruvate are disclosed herein. Methods of culturing these cyanobacteria to produce AKG or pyruvate and recover AKG or pyruvate from the culture are also described herein. Nucleic acid sequences encoding polypeptides that function as ethylene-forming enzymes and their use in the production of ethylene are further disclosed herein. These nucleic acids may be expressed in hosts such as cyanobacteria, which in turn may be cultured to produce ethylene.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
9,309,541
Application Number:
14/643,788
OSTI ID:
1246901
Resource Relation:
Patent File Date: 2015 Mar 10
Country of Publication:
United States
Language:
English

References (29)

Glycogen content and nitrogenase activity in Anabaena variabilis journal December 1984
Heterologous expression of the gene for the ethylene-forming enzyme from Pseudomonas syringae in the cyanobacterium Synechococcus journal January 1994
Changes in photosynthesis and pigmentation in an agp deletion mutant of the cyanobacterium Synechocystis sp. journal January 2003
Sucrose accumulation in salt-stressed cells of agp gene deletion-mutant in cyanobacterium Synechocystis sp. PCC 6803 journal January 2003
Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae journal January 1997
Carbohydrate Metabolism in Mutants of the Cyanobacterium Synechococcus elongatus PCC 7942 Defective in Glycogen Synthesis journal April 2010
Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus journal January 2003
Modification of carbon partitioning to enhance PHB production in Synechocystis sp. PCC6803 journal May 2002
Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins patent August 1990
DNA fragment comprising a gene encoding ethylene forming enzyme of bacteria and the use thereof patent July 1996
Ethanol production by microorganisms patent June 2011
Engineered CO2 fixing microorganisms producing carbon-based products of interest patent July 2011
Ethanol production in microorganisms patent November 2011
Selection of ADH in genetically modified cyanobacteria for the production of ethanol patent April 2012
Ethanol production in microorganisms patent July 2012
Engineered CO2 fixing microorganisms producing carbon-based products of interest patent July 2012
Ethanol production in microorganisms patent June 2013
Genetically Modified Cyanobacteria for the Production of Ethanol patent-application March 2010
Modified Photosynthetic Microorganisms With Reduced Glycogen and Their Use in Producing Carbon-Based Products patent-application July 2010
Genetically Modified Photoautotrophic Ethanol Producing Host Cells, Method For Producing The Host Cells, Constructs For The Transformation Of The Host Cells, Method For Testing A Photoautotrophic Strain For A Desired Growth Property And Method Of Producing Ethanol Using The Host Cells patent-application November 2010
Molecular Approaches for the Optimization of Biofuel Production patent-application December 2010
Designer Oxyphotobacteria and Greenhouse Distillation for Photobiological Ethanol Production from Carbon Dioxide and Water patent-application December 2010
Engineered CO2 Fixing Microorganisms Producing Carbon-Based Products of Interest patent-application January 2011
Modified Photosynthetic Microorganisms for Producing Lipids patent-application October 2011
Engineered CO2 Fixing Microorganisms Producing Carbon-Based Products of Interest patent-application May 2012
Biofuel From Recombinant Oleaginous Algae Using Sugar Carbon Sources patent-application June 2012
Genetically Engineered Cyanobacteria patent-application November 2012
Metabolically Engineered Organisms for the Production of Added Value Bio-Products patent-application May 2013
Biological Production of Organic Compounds patent-application August 2013

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY