Dark States in the Light-Harvesting complex 2 Revealed by Two-dimensional Electronic Spectroscopy

Ferretti, Marco; Hendrikx, Ruud; Romero, Elisabet; Southall, June; Cogdell, Richard J.; Novoderezhkin, Vladimir I.; Scholes, Gregory D.; van Grondelle, Rienk

doi:10.1038/srep20834

Title: Dark States in the Light-Harvesting complex 2 Revealed by Two-dimensional Electronic Spectroscopy

Journal Article · Tue Feb 09 00:00:00 EST 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep20834· OSTI ID:1242364

Ferretti, Marco ^[1]; Hendrikx, Ruud ^[1]; Romero, Elisabet ^[1]; Southall, June ^[2]; Cogdell, Richard J. ^[2]; Novoderezhkin, Vladimir I. ^[3]; Scholes, Gregory D. ^[4]; van Grondelle, Rienk ^[1]

Vrije Univ., Amsterdam (Netherlands). Department of Physics and Astronomy
Univ. of Glasgow, Scotland (United Kingdom). Division of Biochemistry and Molecular Biology
Moscow State Univ., Moscow (Russia). A. N. Belozersky Institute of Physico-Chemical Biology
Princeton Univ., NJ (United States). Dept. of Chemistry

Energy transfer and trapping in the light harvesting antennae of purple photosynthetic bacteria is an ultrafast process, which occurs with a quantum efficiency close to unity. However the mechanisms behind this process have not yet been fully understood. Recently it was proposed that low-lying energy dark states, such as charge transfer states and polaron pairs, play an important role in the dynamics and directionality of energy transfer. However, it is difficult to directly detect those states because of their small transition dipole moment and overlap with the B850/B870 exciton bands. Here we present a new experimental approach, which combines the selectivity of two-dimensional electronic spectroscopy with the availability of genetically modified light harvesting complexes, to reveal the presence of those dark states in both the genetically modified and the wild-type light harvesting 2 complexes of Rhodopseudomonas palustris. In conclusion, we suggest that Nature has used the unavoidable charge transfer processes that occur when LH pigments are concentrated to enhance and direct the flow of energy.

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Research Organization:: Vrije Univ., Amsterdam (Netherlands); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001035

OSTI ID:: 1242364

Journal Information:: Scientific Reports, Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 61 works

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