Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

de-Bashan, Luz E.; Mayali, Xavier; Bebout, Brad M.; Weber, Peter K.; Detweiler, Angela M.; Hernandez, Juan- Pablo; Prufert-Bebout, Leslie; Bashan, Yoav

doi:10.1016/j.algal.2016.02.019

Title: Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

Journal Article · Thu Mar 03 00:00:00 EST 2016 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2016.02.019· OSTI ID:1241994

de-Bashan, Luz E. ^[1]; Mayali, Xavier ^[2]; Bebout, Brad M. ^[3]; Weber, Peter K. ^[2]; Detweiler, Angela M. ^[4];

^[5]; Prufert-Bebout, Leslie ^[6]; Bashan, Yoav ^[1]

The Bashan Institute of Science, Auburn, AL (United States); Auburn Univ., AL (United States); Environmental Microbiology Group, Northwestern center for Biological Research, La Paz (Mexico)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States); Bay Area Environmental Research Inst. Petaluma, CA (United States)
The Bashan Institute of Science, Auburn, AL (United States); Environmental Microbiology Group, Northwestern center for Biological Research, La Paz (Mexico)
The Bashan Institute of Science, Auburn, AL (United States)

The demonstration of a mutualistic interaction requires evidence of benefits for both partners as well as stability of the association over multiple generations. A synthetic mutualism between the freshwater microalga Chlorella sorokiniana and the soil-derived plant growth-promoting bacterium (PGPB) Azospirillum brasilense was created when both microorganisms were co-immobilized in alginate beads. Using stable isotope enrichment experiments followed by high-resolution secondary ion mass spectrometry (SIMS) imaging of single cells, we demonstrated transfer of carbon and nitrogen compounds between the two partners. Further, using fluorescent in situ hybridization (FISH), mechanical disruption and scanning electron microscopy, we demonstrated the stability of their physical association for a period of 10 days after the aggregated cells were released from the beads. The bacteria significantly enhanced the growth of the microalgae while the microalgae supported growth of the bacteria in a medium where it could not otherwise grow. In conclusion, we propose that this microalga-bacterium association is a true synthetic mutualism independent of co-evolution. (155 words).

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC52-07NA27344; SCW1039

OSTI ID:: 1241994

Alternate ID(s):: OSTI ID: 1356676

Report Number(s):: LLNL-JRNL-675179

Journal Information:: Algal Research, Vol. 15, Issue C; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 47 works

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