Revealing the mechanism of passive transport in lipid bilayers via phonon-mediated nanometre-scale density fluctuations

Zhernenkov, Mikhail; Bolmatov, Dima; Soloviov, Dmitry; Zhernenkov, Kirill; Toperverg, Boris P.; Cunsolo, Alessandro; Bosak, Alexey; Cai, Yong

doi:10.1038/ncomms11575

Title: Revealing the mechanism of passive transport in lipid bilayers via phonon-mediated nanometre-scale density fluctuations

Journal Article · Thu May 12 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms11575· OSTI ID:1237168

Zhernenkov, Mikhail ^[1]; Bolmatov, Dima ^[1]; Soloviov, Dmitry ^[2]; Zhernenkov, Kirill ^[3]; Toperverg, Boris P. ^[4]; Cunsolo, Alessandro ^[1]; Bosak, Alexey ^[5]; Cai, Yong ^[1]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Joint Inst. for Nuclear Research (JINR), Dubna (Russian Federation)
Inst. of Nanoscience and Cryogenics, Grenoble (France)
Petersburg Nuclear Physics Inst. Gatchina (Russia)
European Synchrotron Radiation Facility (ESRF), Grenoble (France)

The passive transport of molecules through a cell membrane relies on thermal motions of the lipids. However, the nature of transmembrane transport and the precise mechanism remain elusive and call for a comprehensive study of phonon excitations. Here we report a high resolution inelastic X-ray scattering study of the in-plane phonon excitations in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine above and below the main transition temperature. In the gel phase, for the first time, we observe low-frequency transverse modes, which exhibit a phonon gap when the lipid transitions into the fluid phase. We argue that the phonon gap signifies the formation of short-lived nanometre-scale lipid clusters and transient pores, which facilitate the passive molecular transport across the bilayer plane. Finally, our findings suggest that the phononic motion of the hydrocarbon tails provides an effective mechanism of passive transport, and illustrate the importance of the collective dynamics of biomembranes.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Russian Foundation for Basic Research

Grant/Contract Number:: SC0012704

OSTI ID:: 1237168

Alternate ID(s):: OSTI ID: 1340385

Report Number(s):: BNL-108276-2015-JA; BNL-112571-2016-JA; R&D Project: LS001

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 52 works

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