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Title: A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources

Journal Article · · Journal of Synchrotron Radiation (Online)
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  1. Diamond Light Source, Oxfordshire (United Kingdom)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Univ. of Toronto, Toronto, ON (Canada)
  4. Univ. of Toronto, Toronto, ON (Canada); The Max Planck Institute for the Structure and Dynamics of Matter, and Hamburg Centre for Ultrafast Imaging, Hamburg (Germany)
+ Show Author Affiliations

The design and implementation of a compact and portable sample alignment system suitable for use at both synchrotron and free-electron laser (FEL) sources and its performance are described. The system provides the ability to quickly and reliably deliver large numbers of samples using the minimum amount of sample possible, through positioning of fixed target arrays into the X-ray beam. The combination of high-precision stages, high-quality sample viewing, a fast controller and a software layer overcome many of the challenges associated with sample alignment. A straightforward interface that minimizes setup and sample changeover time as well as simplifying communication with the stages during the experiment is also described, together with an intuitive naming convention for defining, tracking and locating sample positions. Lastly, the setup allows the precise delivery of samples in predefined locations to a specific position in space and time, reliably and simply.

Research Organization:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1234721
Journal Information:
Journal of Synchrotron Radiation (Online), Vol. 22, Issue 6; ISSN 1600-5775
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 43 works
Citation information provided by
Web of Science

References (20)

Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams journal January 2015
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Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams journal April 2015
SEM imaging of liquid jets journal June 2009
Low- Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography journal June 2015
Serial crystallography on in vivo grown microcrystals using synchrotron radiation journal February 2014
Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction journal August 2014
Determination of damage-free crystal structure of an X-ray–sensitive protein using an XFEL journal May 2014
Structure of class B GPCR corticotropin-releasing factor receptor 1 journal July 2013
Fixed-target protein serial microcrystallography with an x-ray free electron laser journal August 2014
Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array journal March 2015
Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography journal August 2015
Rational Engineering of Recombinant Picornavirus Capsids to Produce Safe, Protective Vaccine Antigen journal March 2013
A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering journal May 2015
Nanoflow electrospinning serial femtosecond crystallography journal October 2012
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography journal February 2014
Crystallography on a chip journal February 2012

Cited By (16)

High-speed fixed-target serial virus crystallography journal June 2017
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate journal November 2017
Towards in cellulo virus crystallography journal February 2018
Microfluidics: From crystallization to serial time-resolved crystallography journal May 2017
Successful sample preparation for serial crystallography experiments journal November 2019
Reducing sample consumption for serial crystallography using acoustic drop ejection journal August 2019
Fixed-target serial oscillation crystallography at room temperature journal February 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment journal January 2020
Sample delivery for serial crystallography at free-electron lasers and synchrotrons journal January 2019
Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering text January 2016
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography text January 2018
A fixed-target platform for serial femtosecond crystallography in a hydrated environment text January 2020
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography journal October 2018
Fixed-target serial oscillation crystallography at room temperature text January 2019
Strategies for sample delivery for femtosecond crystallography text January 2019
Fixed-target serial oscillation crystallography at room temperature text January 2019

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07 ISOTOPE AND RADIATION SOURCES
fixed-target serial femtosecond crystallography
portable endstation
motion control
sample delivery