Structural basis for corepressor assembly by the orphan nuclear receptor TLX

Zhi, Xiaoyong; Zhou, X. Edward; He, Yuanzheng; Searose-Xu, Kelvin; Zhang, Chun-Li; Tsai, Chih-Cheng; Melcher, Karsten; Xu, H. Eric

doi:10.1101/gad.254904.114

Title: Structural basis for corepressor assembly by the orphan nuclear receptor TLX

Journal Article · Sun Feb 15 00:00:00 EST 2015 · Genes & Development

DOI:https://doi.org/10.1101/gad.254904.114· OSTI ID:1229906

Zhi, Xiaoyong ^[1]; Zhou, X. Edward ^[2]; He, Yuanzheng ^[2]; Searose-Xu, Kelvin ^[2]; Zhang, Chun-Li ^[3]; Tsai, Chih-Cheng ^[4]; Melcher, Karsten ^[2]; Xu, H. Eric ^[5]

Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Sciences; Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Autophagy Research Center
Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Sciences
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Dept. of Molecular Biology
Univ. of California, Riverside, CA (United States). Dept. of Cell Biology and Neuroscience
Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Sciences; Chinese Academy of Sciences (CAS), Shanghai (China). Shanghai Inst. of Materia Medica. Key Lab. of Receptor Research. Van Andel Research Inst.-Shanghai Inst. of Materia Medica (VARI/SIMM) Center

The orphan nuclear receptor TLX regulates neural stem cell self-renewal in the adult brain and functions primarily as a transcription repressor through recruitment of Atrophin corepressors, which bind to TLX via a conserved peptide motif termed the Atro box. Here we report crystal structures of the human and insect TLX ligand-binding domain in complex with Atro box peptides. In these structures, TLX adopts an autorepressed conformation in which its helix H12 occupies the coactivator-binding groove. Unexpectedly, H12 in this autorepressed conformation forms a novel binding pocket with residues from helix H3 that accommodates a short helix formed by the conserved ALXXLXXY motif of the Atro box. Mutations that weaken the TLX–Atrophin interaction compromise the repressive activity of TLX, demonstrating that this interaction is required for Atrophin to confer repressor activity to TLX. Moreover, the autorepressed conformation is conserved in the repressor class of orphan nuclear receptors, and mutations of corresponding residues in other members of this class of receptors diminish their repressor activities. Together, our results establish the functional conservation of the autorepressed conformation and define a key sequence motif in the Atro box that is essential for TLX-mediated repression.

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Research Organization:: Van Andel Research Inst. (VARI), Grand Rapids, MI (United States); Chinese Academy of Sciences (CAS), Shanghai (China)

Sponsoring Organization:: USDOE Office of Science (SC); National Inst. of Health (NIH) (United States); Michigan Economic Development Corporation (United States); Michigan Technology Tri-Corridor (United States); Jay and Betty Van Andel Foundation (United States); Ministry of Science and Technology (China)

Contributing Organization:: Univ. of Texas Southwestern Medical Center, Dallas, TX (United States); Univ. of California, Riverside, CA (United States)

Grant/Contract Number:: DK071662; NS070981; NS088095; GM102545; GM104212; 085P1000817; 2012ZX09301001; 2012CB910403; 2013CB910600; XDB08020303; 2013ZX09507001

OSTI ID:: 1229906

Journal Information:: Genes & Development, Vol. 29, Issue 4; ISSN 0890-9369

Publisher:: Cold Springs Harbor PressCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 17 works

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