Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals

Dao, E. Han; Sierra, Raymond G.; Laksmono, Hartawan; Lemke, Henrik T.; Alonso-Mori, Roberto; Coey, Aaron; Larsen, Kevin; Baxter, Elizabeth L.; Cohen, Aina E.; Soltis, S. Michael; DeMirci, Hasan

doi:10.1063/1.4919407

Title: Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals

Journal Article · Thu Apr 30 00:00:00 EDT 2015 · Structural Dynamics

DOI:https://doi.org/10.1063/1.4919407· OSTI ID:1222953

Dao, E. Han ^[1]; Sierra, Raymond G. ^[1]; Laksmono, Hartawan ^[1]; Lemke, Henrik T. ^[1]; Alonso-Mori, Roberto ^[1]; Coey, Aaron ^[2]; Larsen, Kevin ^[2]; Baxter, Elizabeth L. ^[1]; Cohen, Aina E. ^[1]; Soltis, S. Michael ^[1]; DeMirci, Hasan ^[1]

SLAC National Accelerator Lab., Menlo Park, CA (United States)
Stanford Univ., CA (United States)

In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit crystals were genetically engineered to omit a 26-residue protein, Thx, which is present in the wild-type Thermus thermophilus 30S ribosomal subunit. We are primarily interested in elucidating the contribution of this ribosomal protein to the overall 30S subunit structure. To assess the viability of this study, femtosecond X-ray diffraction patterns from these crystals were recorded at the LCLS during a protein crystal screening beam time. During our data collection, we successfully observed diffraction from these difficult-to-grow 30S ribosomal subunit crystals. Most of our crystals were found to diffract to low resolution, while one crystal diffracted to 3.2 Å resolution. These data suggest the feasibility of pursuing high-resolution data collection as well as the need to improve sample preparation and handling in order to collect a complete radiation-damage-free data set using an X-ray Free Electron Laser.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1222953

Journal Information:: Structural Dynamics, Vol. 2, Issue 4; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 1 work

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